Restriction map for
pFN21AE4529
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1150 GACGTC GACGT^C
AlwNI 403 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1342 GGGCCC GGGCC^C
Asp700I 115 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1555 GGCGCC GGCGC^C
BbrPI 520 CACGTG CAC^GTG
BclI 776 TGATCA T^GATCA
BglI 1435 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1527 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1527 GCTCAGC GC^T[ACGT]AGC
BsaAI 520 CACGTG [CT]AC^GT[GA]
BsgI 232 GTGCAG GTGCAG(16/14)
BsmBI 130 CGTCTC CGTCTC(1/5)
BsmI 1722 GCATTC GAATGC(1/-1)
BspLU11I 1415 ACATGT A^CATGT
BspMI 922 ACCTGC ACCTGC(4/8)
BstEII 460 GGTGACC G^GT[ACGT]ACC
BstPI 460 GGTGACC G^GT[ACGT]ACC
CelII 1527 GCTCAGC GC^T[ACGT]AGC
DraIII 797 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 904 GACGTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 530 CGGCCA [CT]^GGCC[GA]
EcoO65I 460 GGTGACC G^GT[ACGT]ACC
FbaI 776 TGATCA T^GATCA
HaeII 1555 GGCGCC [GA]GCGC^[CT]
HinfI 150 GAGTC G^A[ACGT]TC
KasI 1555 GGCGCC G^GCGCC
KspI 1662 CCGCGG CCGC^GG
MseI 719 TTAA T^TAA
NarI 1555 GGCGCC GG^CGCC
NcoI 1373 CCATGG C^CATGG
NspI 1415 ACATGT [GA]CATG^[CT]
PleI 150 GAGTC GAGTC(4/5)
PmaCI 520 CACGTG CAC^GTG
PmlI 520 CACGTG CAC^GTG
SacII 1662 CCGCGG CCGC^GG
SapI 1473 GCTCTTC GCTCTTC(1/4)
SfoI 1555 GGCGCC GGC^GCC
SgrAI 1551 CACCGGCG C[GA]^CCGG[CT]G
SmaI 58 CCCGGG CCC^GGG
StuI 1613 AGGCCT AGG^CCT
Tru9I 719 TTAA T^TAA
XcmI 1247 CCACACTGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
XmnI 115 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 670 AAATTC [GA]^AATT[CT]
1045 GAATTT
AcyI 1150 GACGTC G[GA]^CG[CT]C
1555 GGCGCC
AflIII 422 ACGTGT A^C[GA][CT]GT
1415 ACATGT
ApoI 670 AAATTC [GA]^AATT[CT]
1045 GAATTT
AspHI 1087 GTGCTC G[AT]GC[AT]^C
1525 GTGCTC
BamHI 585 GGATCC G^GATCC
809 GGATCC
BanII 250 GGGCTC G[GA]GC[CT]^C
1342 GGGCCC
BsaBI 810 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1116 GATCGGCATC
BsaHI 1150 GACGTC G[GA]^CG[CT]C
1555 GGCGCC
BsaI 31 GAGACC GGTCTC(1/5)
826 GAGACC
BsiHKAI 1087 GTGCTC G[AT]GC[AT]^C
1525 GTGCTC
BspHI 146 TCATGA T^CATGA
1109 TCATGA
BstAPI 182 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1722 GCATTCGCTGC
BstXI 815 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1130 CCAAGAACTTGG
Hin1I 1150 GACGTC G[GA]^CG[CT]C
1555 GGCGCC
MamI 810 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1116 GATCGGCATC
PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT]
1706 GGGACCC
PvuII 262 CAGCTG CAG^CTG
370 CAGCTG
RcaI 146 TCATGA T^CATGA
1109 TCATGA
Tsp509I 671 AATT ^AATT
1046 AATT
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XhoII