Restriction map for
pFN21AE5304
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BciVI BclI BglI
BglII BlnI BmyI BpmI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 111 GAATTT [GA]^AATT[CT]
AfaI 303 GTAC GT^AC
AflII 286 CTTAAG C^TTAAG
ApoI 111 GAATTT [GA]^AATT[CT]
AvaI 347 CCCGGG C^[CT]CG[GA]G
BamHI 483 GGATCC G^GATCC
BcgI 596 GCACCACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 577 CTAG C^TAG
BfrI 286 CTTAAG C^TTAAG
BsgI 594 CTGCAC GTGCAG(16/14)
BsoBI 347 CCCGGG C^[CT]CG[GA]G
BspMI 592 ACCTGC ACCTGC(4/8)
BsrDI 716 GCAATG GCAATG(2/0)
CfoI 638 GCGC GCG^C
DraIII 475 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 566 GACTGGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 390 GAAGAG CTCTTC(1/4)
HaeII 637 GGCGCT [GA]GCGC^[CT]
HhaI 638 GCGC GCG^C
HinP1I 638 GCGC G^CGC
HincII 418 GTTAAC GT[CT]^[GA]AC
HindII 418 GTTAAC GT[CT]^[GA]AC
HinfI 84 GAATC G^A[ACGT]TC
HpaI 418 GTTAAC GTT^AAC
Ksp632I 390 GAAGAG CTCTTC(1/4)
MaeI 577 CTAG C^TAG
PflMI 252 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 303 GTAC GT^AC
SmaI 347 CCCGGG CCC^GGG
SmlI 286 CTTAAG C^T[CT][GA]AG
StuI 103 AGGCCT AGG^CCT
TfiI 84 GAATC G^A[AT]TC
Van91I 252 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 379 GACGTC GACGT^C
572 GACGTC
AcyI 379 GACGTC G[GA]^CG[CT]C
572 GACGTC
AhdI 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
572 GACGTCTAGTC
AlwNI 253 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
334 CAGAGACTG
AspEI 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
572 GACGTCTAGTC
BcnI 347 CCCGG CC^[GC]GG
348 CCGGG
BlpI 454 GCTCAGC GC^T[ACGT]AGC
646 GCTAAGC
Bpu1102I 454 GCTCAGC GC^T[ACGT]AGC
646 GCTAAGC
BsaHI 379 GACGTC G[GA]^CG[CT]C
572 GACGTC
BsaI 146 GAGACC GGTCTC(1/5)
393 GAGACC
BsiYI 252 CCAGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCGTCACTGGG
BslI 252 CCAGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
702 CCGTCACTGGG
BstF5I 132 CATCC GGATG(2/0)
204 CATCC
BstYI 483 GGATCC [GA]^GATC[CT]
695 AGATCC
CelII 454 GCTCAGC GC^T[ACGT]AGC
646 GCTAAGC
DpnII 484 GATC ^GATC
696 GATC
Eam1105I 395 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
572 GACGTCTAGTC
EcoT14I 20 CCAAGG C^C[AT][AT]GG
434 CCTTGG
FauI 155 CCCGC CCCGC(4/6)
173 CCCGC
FokI 132 CATCC GGATG(9/13)
204 CATCC
HapII 40 CCGG C^CGG
348 CCGG
Hin1I 379 GACGTC G[GA]^CG[CT]C
572 GACGTC
HpaII 40 CCGG C^CGG
348 CCGG
MaeIII 427 GTCAC ^GT[ACGT]AC
704 GTCAC
MboI 484 GATC ^GATC
696 GATC
MflI 483 GGATCC [GA]^GATC[CT]
695 AGATCC
MspI 40 CCGG C^CGG
348 CCGG
NciI 347 CCCGG CC^[GC]GG
348 CCGGG
NdeII 484 GATC ^GATC
696 GATC
PpuMI 533 GGGTCCC [GA]G^G[AT]CC[CT]
726 GGGTCCT
Sau3AI 484 GATC ^GATC
696 GATC
StyI 20 CCAAGG C^C[AT][AT]GG
434 CCTTGG
Tsp45I 427 GTCAC ^GT[GC]AC
704 GTCAC
Tsp509I 112 AATT ^AATT
673 AATT
TspRI 600 CACTG CA[GC]TG[ACGT][ACGT]^
706 CACTG
XhoII 483 GGATCC [GA]^GATC[CT]
695 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwI AvaII BbsI
BbvI BpuAI BsaJI BsmAI BsmFI
BsrI BssKI BstNI BstXI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HphI ItaI MaeII
MboII MnlI MseI MslI MspA1I
MvaI MwoI NlaIV PvuII Sau96I
ScrFI TaqI Tru9I TseI TthHB8I