Restriction map for
pFN21AE4421
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BanI BbeI BcgI BclI
BcnI BfrI BglI BglII BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI Hin1I
HpaII KasI KpnI Ksp632I KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 669 GTCTAC GT^[AC][GT]AC
AhdI 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 431 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 41 CTCGGG C^[CT]CG[GA]G
BamHI 661 GGATCC G^GATCC
BanII 66 GAGCTC G[GA]GC[CT]^C
BbrPI 969 CACGTG CAC^GTG
BciVI 117 GTATCC GTATCC(6/5)
BpmI 961 CTCCAG CTGGAG(16/14)
BsaI 1282 GAGACC GGTCTC(1/5)
BsgI 1256 GTGCAG GTGCAG(16/14)
BsmI 1239 GAATGC GAATGC(1/-1)
BsoBI 41 CTCGGG C^[CT]CG[GA]G
BspMI 53 ACCTGC ACCTGC(4/8)
BsrDI 1073 GCAATG GCAATG(2/0)
BssSI 511 CTCGTG CACGAG(-5/-1)
BstYI 661 GGATCC [GA]^GATC[CT]
CfoI 329 GCGC GCG^C
DraI 1087 TTTAAA TTT^AAA
DraII 1206 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 176 CCGTGG C^C[GA][CT]GG
Eam1105I 79 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1206 AGGTCCC [GA]G^G[ACGT]CC[CT]
FauI 941 CCCGC CCCGC(4/6)
HaeII 328 GGCGCT [GA]GCGC^[CT]
HhaI 329 GCGC GCG^C
HinP1I 329 GCGC G^CGC
HindIII 676 AAGCTT A^AGCTT
HpaI 1177 GTTAAC GTT^AAC
MfeI 935 CAATTG C^AATTG
MflI 661 GGATCC [GA]^GATC[CT]
MunI 935 CAATTG C^AATTG
PmaCI 969 CACGTG CAC^GTG
PmlI 969 CACGTG CAC^GTG
PpuMI 1206 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 1132 CTGCAG CTGCA^G
SacI 66 GAGCTC GAGCT^C
SfcI 1132 CTGCAG C^T[GA][CT]AG
XhoII 661 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 781 GAATTT [GA]^AATT[CT]
818 AAATTC
ApoI 781 GAATTT [GA]^AATT[CT]
818 AAATTC
AspI 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1227 GACTGAGTC
BalI 179 TGGCCA TGG^CCA
389 TGGCCA
BbsI 162 GAAGAC GAAGAC(2/6)
450 GAAGAC
BbvCI 89 CCTCAGC CCTCAGC(-5/-2)
1219 CCTCAGC
BlpI 724 GCTAAGC GC^T[ACGT]AGC
907 GCTGAGC
Bpu1102I 724 GCTAAGC GC^T[ACGT]AGC
907 GCTGAGC
BpuAI 162 GAAGAC GAAGAC(2/6)
450 GAAGAC
BseRI 1108 CTCCTC GAGGAG(10/8)
271 GAGGAG
BsmFI 897 GTCCC GGGAC(10/14)
1208 GTCCC
BssKI 788 CCAGG ^CC[ACGT]GG
1278 CCAGG
BstAPI 4 GCATCCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
343 GCAAAAGATGC
BstNI 788 CCAGG CC^[AT]GG
1278 CCAGG
BstXI 455 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1028 CCAAGCACATGG
CelII 724 GCTAAGC GC^T[ACGT]AGC
907 GCTGAGC
EaeI 179 TGGCCA [CT]^GGCC[GA]
389 TGGCCA
EcoRII 788 CCAGG ^CC[AT]GG
1278 CCAGG
HincII 49 GTCAAC GT[CT]^[GA]AC
1177 GTTAAC
HindII 49 GTCAAC GT[CT]^[GA]AC
1177 GTTAAC
MluNI 179 TGGCCA TGG^CCA
389 TGGCCA
MscI 179 TGGCCA TGG^CCA
389 TGGCCA
MspA1I 1129 CAGCTG C[AC]G^C[GT]G
1222 CAGCTG
MvaI 788 CCAGG CC^[AT]GG
1278 CCAGG
PflFI 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1227 GACTGAGTC
PvuII 1129 CAGCTG CAG^CTG
1222 CAGCTG
ScrFI 788 CCAGG CC^[ACGT]GG
1278 CCAGG
Tth111I 453 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1227 GACTGAGTC
XcmI 167 CCATCACCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
377 CCAAGGATTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BfaI BmyI BsaAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BstF5I Cac8I Cfr13I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI