Restriction map for
pFN21AE5111
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# NO CUT ENZYMES ( 198 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BbvI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstAPI BstBI BstNI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
ItaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MscI MslI MunI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI ScrFI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TseI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 228 TCCGGA T^CCGGA
AciI 5 CCGC CCGC(-3/-1)
AcsI 101 GAATTC [GA]^AATT[CT]
AfaI 212 GTAC GT^AC
AlwI 146 GGATC GGATC(4/5)
ApoI 101 GAATTC [GA]^AATT[CT]
Asp700I 241 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 123 GAAGAC GAAGAC(2/6)
BcgI 30 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 175 CTAG C^TAG
BpuAI 123 GAAGAC GAAGAC(2/6)
BsaJI 141 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 228 TCCGGA [AT]^CCGG[AT]
BseAI 228 TCCGGA T^CCGGA
BsiYI 229 CCGGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 229 CCGGAATATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspEI 228 TCCGGA T^CCGGA
BspMI 62 ACCTGC ACCTGC(4/8)
BsrDI 206 GCAATG GCAATG(2/0)
BstEII 259 GGTGACC G^GT[ACGT]ACC
BstF5I 226 CATCC GGATG(2/0)
BstPI 259 GGTGACC G^GT[ACGT]ACC
EcoO65I 259 GGTGACC G^GT[ACGT]ACC
EcoRI 101 GAATTC G^AATTC
EcoT14I 141 CCAAGG C^C[AT][AT]GG
FokI 226 CATCC GGATG(9/13)
HapII 229 CCGG C^CGG
HpaII 229 CCGG C^CGG
MaeI 175 CTAG C^TAG
MaeIII 260 GTGAC ^GT[ACGT]AC
MboII 123 GAAGA GAAGA(8/7)
MroI 228 TCCGGA T^CCGGA
MseI 188 TTAA T^TAA
MspA1I 319 CAGCTG C[AC]G^C[GT]G
MspI 229 CCGG C^CGG
NdeI 276 CATATG CA^TATG
NlaIII 331 CATG CATG^
PvuII 319 CAGCTG CAG^CTG
RsaI 212 GTAC GT^AC
SfcI 253 CTACAG C^T[GA][CT]AG
SpeI 174 ACTAGT A^CTAGT
StyI 141 CCAAGG C^C[AT][AT]GG
TaqI 29 TCGA T^CGA
Tru9I 188 TTAA T^TAA
Tsp45I 260 GTGAC ^GT[GC]AC
TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 29 TCGA T^CGA
XmnI 241 GAAGTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AluI 251 AGCT AG^CT
320 AGCT
BsrI 303 ACTGG ACTGG(1/-1)
282 CCAGT
Cac8I 73 GCTGGC GC[ACGT]^[ACGT]GC
202 GCCTGC
DdeI 80 CTGAG C^T[ACGT]AG
308 CTAAG
DpnII 147 GATC ^GATC
316 GATC
HphI 55 GGTGA GGTGA(8/7)
259 GGTGA
MboI 147 GATC ^GATC
316 GATC
NdeII 147 GATC ^GATC
316 GATC
Sau3AI 147 GATC ^GATC
316 GATC
SfaNI 43 GATGC GCATC(5/9)
225 GCATC
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
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MnlI Tsp509I