Restriction map for
pFN21AB9982
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccIII AclI AflII AgeI AocI
AscI AseI AsnI Asp700I AspI
AvaI AviII AvrII BbrPI BciVI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII BstAPI BstBI Bsu36I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI FspI HindIII KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 25 GACGTC GACGT^C
Acc65I 1144 GGTACC G^GTACC
AccII 1257 CGCG CG^CG
AflIII 914 ACATGT A^C[GA][CT]GT
AhdI 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 616 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 887 AGCGCT AGC^GCT
ApaI 1010 GGGCCC GGGCC^C
Asp718I 1144 GGTACC G^GTACC
AspEI 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 158 GGCGCC GGCGC^C
BbsI 1860 GTCTTC GAAGAC(2/6)
BcgI 1314 CGATGAATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 767 TGATCA T^GATCA
BlpI 1965 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1965 GCTAAGC GC^T[ACGT]AGC
BpuAI 1860 GTCTTC GAAGAC(2/6)
BsaI 1180 GAGACC GGTCTC(1/5)
BsgI 667 CTGCAC GTGCAG(16/14)
BsmI 1395 GAATGC GAATGC(1/-1)
BspHI 950 TCATGA T^CATGA
BspLU11I 914 ACATGT A^CATGT
BspMI 671 ACCTGC ACCTGC(4/8)
BsrBI 1066 GAGCGG CCGCTC(-3/-3)
BsrDI 401 GCAATG GCAATG(2/0)
Bst1107I 1200 GTATAC GTA^TAC
BstEII 370 GGTGACC G^GT[ACGT]ACC
BstPI 370 GGTGACC G^GT[ACGT]ACC
BstUI 1257 CGCG CG^CG
BstXI 510 CCAGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1200 GTATAC GTA^TAC
CelII 1965 GCTAAGC GC^T[ACGT]AGC
DrdI 373 GACCAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2104 GACTCAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 887 AGCGCT AGC^GCT
EcoO65I 370 GGTGACC G^GT[ACGT]ACC
EcoRI 445 GAATTC G^AATTC
FbaI 767 TGATCA T^GATCA
HpaI 141 GTTAAC GTT^AAC
KasI 158 GGCGCC G^GCGCC
KpnI 1144 GGTACC GGTAC^C
MvnI 1257 CGCG CG^CG
NarI 158 GGCGCC GG^CGCC
NcoI 177 CCATGG C^CATGG
PpuMI 299 AGGACCT [GA]G^G[AT]CC[CT]
PstI 613 CTGCAG CTGCA^G
PvuII 349 CAGCTG CAG^CTG
RcaI 950 TCATGA T^CATGA
SacI 2010 GAGCTC GAGCT^C
SalI 62 GTCGAC G^TCGAC
SapI 2012 GCTCTTC GCTCTTC(1/4)
SfcI 613 CTGCAG C^T[GA][CT]AG
SfiI 1566 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 158 GGCGCC GGC^GCC
StuI 536 AGGCCT AGG^CCT
TfiI 235 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 337 AAATTT [GA]^AATT[CT]
445 GAATTC
AcyI 25 GACGTC G[GA]^CG[CT]C
158 GGCGCC
Alw44I 414 GTGCAC G^TGCAC
1099 GTGCAC
ApaLI 414 GTGCAC G^TGCAC
1099 GTGCAC
ApoI 337 AAATTT [GA]^AATT[CT]
445 GAATTC
BalI 204 TGGCCA TGG^CCA
1574 TGGCCA
BanI 158 GGCGCC G^G[CT][GA]CC
1144 GGTACC
BbvCI 396 CCTCAGC CCTCAGC(-5/-2)
1798 GCTGAGG
BglI 77 GCCGTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1567 GCCAAGATGGC
BglII 1118 AGATCT A^GATCT
1748 AGATCT
BsaHI 25 GACGTC G[GA]^CG[CT]C
158 GGCGCC
BssSI 629 CTCGTG CACGAG(-5/-1)
1139 CTCGTG
DraII 299 AGGACCT [GA]G^G[ACGT]CC[CT]
1009 GGGGCCC
DsaI 177 CCATGG C^C[GA][CT]GG
581 CCACGG
EaeI 204 TGGCCA [CT]^GGCC[GA]
1574 TGGCCA
EcoO109I 299 AGGACCT [GA]G^G[ACGT]CC[CT]
1009 GGGGCCC
FauI 1059 CCCGC CCCGC(4/6)
1068 GCGGG
Hin1I 25 GACGTC G[GA]^CG[CT]C
158 GGCGCC
MluNI 204 TGGCCA TGG^CCA
1574 TGGCCA
MscI 204 TGGCCA TGG^CCA
1574 TGGCCA
MseI 142 TTAA T^TAA
185 TTAA
MslI 480 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
670 CACCTGCATG
MspA1I 349 CAGCTG C[AC]G^C[GT]G
1060 CCGCTG
NspI 914 ACATGT [GA]CATG^[CT]
1661 ACATGC
ScaI 714 AGTACT AGT^ACT
2083 AGTACT
SmlI 283 CTTGAG C^T[CT][GA]AG
1956 CTCAAG
TaqI 63 TCGA T^CGA
1112 TCGA
Tru9I 142 TTAA T^TAA
185 TTAA
TthHB8I 63 TCGA T^CGA
1112 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AspHI AvaII BamHI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I Tsp509I TspRI
XhoII