Restriction map for
pFN21AE1354
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI Asp700I Asp718I AvrII BbrPI
BbsI BciVI BclI BfaI BfrI
BlnI BpuAI BsaAI BsaBI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrGI Bst1107I BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SapI
SbfI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2452 GTCGAC GT^[AC][GT]AC
AflIII 1658 ACATGT A^C[GA][CT]GT
AocI 450 CCTTAGG CC^T[ACGT]AGG
Aor51HI 342 AGCGCT AGC^GCT
AvaI 2359 CCCGAG C^[CT]CG[GA]G
AviII 1392 TGCGCA TGC^GCA
BbeI 2649 GGCGCC GGCGC^C
BglII 1490 AGATCT A^GATCT
BlpI 2058 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2058 GCTGAGC GC^T[ACGT]AGC
BsaWI 836 ACCGGA [AT]^CCGG[AT]
BsiEI 2409 CGACCG CG[GA][CT]^CG
BsoBI 2359 CCCGAG C^[CT]CG[GA]G
BspHI 2441 TCATGA T^CATGA
BspLU11I 1658 ACATGT A^CATGT
BssHII 1605 GCGCGC G^CGCGC
BstAPI 1881 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 682 TTCGAA TT^CGAA
BstEII 284 GGTTACC G^GT[ACGT]ACC
BstPI 284 GGTTACC G^GT[ACGT]ACC
Bsu36I 450 CCTTAGG CC^T[ACGT]AGG
CelII 2058 GCTGAGC GC^T[ACGT]AGC
DraIII 1476 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 342 AGCGCT AGC^GCT
Eco81I 450 CCTTAGG CC^T[ACGT]AGG
EcoO65I 284 GGTTACC G^GT[ACGT]ACC
FspI 1392 TGCGCA TGC^GCA
HinfI 1459 GACTC G^A[ACGT]TC
KasI 2649 GGCGCC G^GCGCC
NarI 2649 GGCGCC GG^CGCC
NspI 1658 ACATGT [GA]CATG^[CT]
NspV 682 TTCGAA TT^CGAA
PflMI 935 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1459 GACTC GAGTC(4/5)
PstI 1078 CTGCAG CTGCA^G
RcaI 2441 TCATGA T^CATGA
SacI 1435 GAGCTC GAGCT^C
SalI 2452 GTCGAC G^TCGAC
ScaI 2630 AGTACT AGT^ACT
SexAI 2537 ACCAGGT A^CC[AT]GGT
SfcI 1078 CTGCAG C^T[GA][CT]AG
SfiI 1755 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2649 GGCGCC GGC^GCC
SfuI 682 TTCGAA TT^CGAA
SmlI 244 CTCAAG C^T[CT][GA]AG
TspRI 1501 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 935 CCATCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 316 GTGCAC G^TGCAC
1021 GTGCAC
AlwNI 988 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1879 CAGCAGCTG
ApaLI 316 GTGCAC G^TGCAC
1021 GTGCAC
AspI 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2536 GACCAGGTC
BalI 902 TGGCCA TGG^CCA
1763 TGGCCA
BamHI 923 GGATCC G^GATCC
1991 GGATCC
BcgI 1383 CGACCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
203 GCACACAGATCG
BsmBI 236 GAGACG CGTCTC(1/5)
1324 GAGACG
BssSI 1273 CACGAG CACGAG(-5/-1)
1366 CACGAG
BstXI 713 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1973 CCAATGTTGTGG
DrdI 652 GACGACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
774 GACCTATGTGTC
EarI 394 GAAGAG CTCTTC(1/4)
481 GAAGAG
Ksp632I 394 GAAGAG CTCTTC(1/4)
481 GAAGAG
MaeII 545 ACGT A^CGT
2399 ACGT
MluNI 902 TGGCCA TGG^CCA
1763 TGGCCA
MscI 902 TGGCCA TGG^CCA
1763 TGGCCA
NcoI 1640 CCATGG C^CATGG
2183 CCATGG
PflFI 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2536 GACCAGGTC
Tth111I 655 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2536 GACCAGGTC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
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AccII AciI AcyI AfaI AhdI
AluI AlwI ApaI AspEI AspHI
AvaII BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StuI StyI TaqI TseI
Tsp45I TthHB8I XcmI XhoII