Restriction map for
pFN21AE6302
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaII AvrII BamHI BbeI BciVI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BseAI BsgI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI KasI Ksp632I
MaeI MaeIII MfeI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcyI 235 GGCGTC G[GA]^CG[CT]C
Aor51HI 170 AGCGCT AGC^GCT
AvaI 72 CCCGGG C^[CT]CG[GA]G
AviII 614 TGCGCA TGC^GCA
BalI 869 TGGCCA TGG^CCA
BbrPI 774 CACGTG CAC^GTG
BbvCI 321 CCTCAGC CCTCAGC(-5/-2)
BcgI 216 GCAGACGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 662 TGATCA T^GATCA
BglII 116 AGATCT A^GATCT
BpmI 751 CTGGAG CTGGAG(16/14)
BsaBI 117 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 235 GGCGTC G[GA]^CG[CT]C
BsaI 430 GAGACC GGTCTC(1/5)
BsaWI 767 ACCGGA [AT]^CCGG[AT]
BseRI 716 CTCCTC GAGGAG(10/8)
BsmAI 430 GAGAC GTCTC(1/5)
BsmI 820 GCATTC GAATGC(1/-1)
BsoBI 72 CCCGGG C^[CT]CG[GA]G
BspHI 602 TCATGA T^CATGA
BssHII 21 GCGCGC G^CGCGC
Eco47III 170 AGCGCT AGC^GCT
FbaI 662 TGATCA T^GATCA
FspI 614 TGCGCA TGC^GCA
Hin1I 235 GGCGTC G[GA]^CG[CT]C
HinfI 488 GATTC G^A[ACGT]TC
MamI 117 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 364 ACGCGT A^CGCGT
MluNI 869 TGGCCA TGG^CCA
MscI 869 TGGCCA TGG^CCA
MseI 731 TTAA T^TAA
MslI 435 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 31 GCGGCCGC GC^GGCCGC
NspI 332 ACATGC [GA]CATG^[CT]
PmaCI 774 CACGTG CAC^GTG
PmlI 774 CACGTG CAC^GTG
PstI 157 CTGCAG CTGCA^G
RcaI 602 TCATGA T^CATGA
SfcI 157 CTGCAG C^T[GA][CT]AG
SmaI 72 CCCGGG CCC^GGG
SmlI 814 CTCAAG C^T[CT][GA]AG
TfiI 488 GATTC G^A[AT]TC
Tru9I 731 TTAA T^TAA
XmaCI 72 CCCGGG C^CCGGG
XmaI 72 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Acc65I 188 GGTACC G^GTACC
623 GGTACC
AflIII 364 ACGCGT A^C[GA][CT]GT
773 ACACGT
AlwI 84 GATCC GGATC(4/5)
795 GATCC
Asp718I 188 GGTACC G^GTACC
623 GGTACC
BanI 188 GGTACC G^G[CT][GA]CC
623 GGTACC
BanII 573 GGGCTC G[GA]GC[CT]^C
811 GGGCTC
BsaAI 142 TACGTG [CT]AC^GT[GA]
774 CACGTG
BsiEI 32 CGGCCG CG[GA][CT]^CG
293 CGGCCG
BstYI 116 AGATCT [GA]^GATC[CT]
794 AGATCC
DdeI 259 CTTAG C^T[ACGT]AG
322 CTCAG
DraII 545 AGGGCCT [GA]G^G[ACGT]CC[CT]
746 AGGGCCT
EagI 32 CGGCCG C^GGCCG
293 CGGCCG
EclXI 32 CGGCCG C^GGCCG
293 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
293 CGGCCG
EcoNI 564 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
798 CCTGAAGAAGG
EcoO109I 545 AGGGCCT [GA]G^G[ACGT]CC[CT]
746 AGGGCCT
HaeII 170 AGCGCT [GA]GCGC^[CT]
388 GGCGCT
KpnI 188 GGTACC GGTAC^C
623 GGTACC
KspI 184 CCGCGG CCGC^GG
252 CCGCGG
MflI 116 AGATCT [GA]^GATC[CT]
794 AGATCC
NcoI 231 CCATGG C^CATGG
425 CCATGG
PvuII 154 CAGCTG CAG^CTG
160 CAGCTG
SacII 184 CCGCGG CCGC^GG
252 CCGCGG
XhoII 116 AGATCT [GA]^GATC[CT]
794 AGATCC
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TspRI TthHB8I