Restriction map for
pFN21AE6402
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsaI RsrII SacI
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 753 TCCGGA T^CCGGA
AocI 214 CCTCAGG CC^T[ACGT]AGG
Aor51HI 944 AGCGCT AGC^GCT
BalI 593 TGGCCA TGG^CCA
BanI 685 GGTGCC G^G[CT][GA]CC
BanII 32 GAGCCC G[GA]GC[CT]^C
BbsI 384 GAAGAC GAAGAC(2/6)
BclI 1073 TGATCA T^GATCA
BcnI 35 CCCGG CC^[GC]GG
BfaI 391 CTAG C^TAG
BglI 220 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 384 GAAGAC GAAGAC(2/6)
BsaBI 168 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 472 GAGACC GGTCTC(1/5)
BsaWI 753 TCCGGA [AT]^CCGG[AT]
BseAI 753 TCCGGA T^CCGGA
BseRI 1246 GAGGAG GAGGAG(10/8)
BsgI 536 GTGCAG GTGCAG(16/14)
BspDI 169 ATCGAT AT^CGAT
BspEI 753 TCCGGA T^CCGGA
BspHI 461 TCATGA T^CATGA
BsrFI 1453 ACCGGC [GA]^CCGG[CT]
Bsu36I 214 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1453 ACCGGC [GA]^CCGG[CT]
ClaI 169 ATCGAT AT^CGAT
DraII 209 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1384 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 593 TGGCCA [CT]^GGCC[GA]
Eco47III 944 AGCGCT AGC^GCT
Eco81I 214 CCTCAGG CC^T[ACGT]AGG
EcoNI 1466 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 209 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 172 GATATC GAT^ATC
FbaI 1073 TGATCA T^GATCA
MaeI 391 CTAG C^TAG
MaeII 261 ACGT A^CGT
MamI 168 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 593 TGGCCA TGG^CCA
MroI 753 TCCGGA T^CCGGA
MscI 593 TGGCCA TGG^CCA
MspA1I 910 CAGCTG C[AC]G^C[GT]G
NciI 35 CCCGG CC^[GC]GG
NdeI 1325 CATATG CA^TATG
PpuMI 209 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 43 CTGCAG CTGCA^G
PvuII 910 CAGCTG CAG^CTG
RcaI 461 TCATGA T^CATGA
SapI 123 GCTCTTC GCTCTTC(1/4)
SbfI 42 CCTGCAGG CCTGCA^GG
SexAI 76 ACCTGGT A^CC[AT]GGT
Sse8387I 42 CCTGCAGG CCTGCA^GG
StuI 420 AGGCCT AGG^CCT
XcmI 224 CCAAGGCAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 139 AAATTC [GA]^AATT[CT]
735 AAATTT
ApoI 139 AAATTC [GA]^AATT[CT]
735 AAATTT
BamHI 38 GGATCC G^GATCC
781 GGATCC
BmyI 32 GAGCCC G[AGT]GC[ACT]^C
686 GTGCCC
Bsp1286I 32 GAGCCC G[AGT]GC[ACT]^C
686 GTGCCC
BsrDI 246 CATTGC GCAATG(2/0)
543 CATTGC
BstYI 38 GGATCC [GA]^GATC[CT]
781 GGATCC
CfoI 7 GCGC GCG^C
945 GCGC
EarI 99 CTCTTC CTCTTC(1/4)
124 CTCTTC
HaeII 6 GGCGCT [GA]GCGC^[CT]
944 AGCGCT
HhaI 7 GCGC GCG^C
945 GCGC
HinP1I 7 GCGC G^CGC
945 GCGC
Ksp632I 99 CTCTTC CTCTTC(1/4)
124 CTCTTC
MflI 38 GGATCC [GA]^GATC[CT]
781 GGATCC
MslI 591 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1422 CATTCCAGTG
NspI 438 GCATGC [GA]CATG^[CT]
1014 GCATGC
PflMI 986 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1383 CCACTTGGTGG
SfcI 43 CTGCAG C^T[GA][CT]AG
444 CTGTAG
SphI 438 GCATGC GCATG^C
1014 GCATGC
TfiI 148 GAATC G^A[AT]TC
679 GATTC
Tsp45I 158 GTGAC ^GT[GC]AC
451 GTGAC
Van91I 986 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1383 CCACTTGGTGG
XhoII 38 GGATCC [GA]^GATC[CT]
781 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BspMI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I