Restriction map for
pFN21AE6203
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbvCI BcgI BclI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmFI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI MaeI MaeIII
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PleI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AspHI 637 GAGCTC G[AT]GC[AT]^C
AspI 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 657 TGGCCA TGG^CCA
BbeI 271 GGCGCC GGCGC^C
BbrPI 214 CACGTG CAC^GTG
BciVI 375 GGATAC GTATCC(6/5)
BcnI 522 CCGGG CC^[GC]GG
BglII 368 AGATCT A^GATCT
BsaAI 214 CACGTG [CT]AC^GT[GA]
BseRI 229 GAGGAG GAGGAG(10/8)
BsiEI 519 CGGCCG CG[GA][CT]^CG
BsiHKAI 637 GAGCTC G[AT]GC[AT]^C
BsmAI 448 GAGAC GTCTC(1/5)
BsmBI 448 GAGACG CGTCTC(1/5)
BsmI 252 GAATGC GAATGC(1/-1)
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BssSI 160 CACGAG CACGAG(-5/-1)
BstXI 496 CCAAACACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 368 AGATCT [GA]^GATC[CT]
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
DdeI 365 CTGAG C^T[ACGT]AG
EagI 519 CGGCCG C^GGCCG
EclXI 519 CGGCCG C^GGCCG
Eco52I 519 CGGCCG C^GGCCG
EcoT14I 329 CCTTGG C^C[AT][AT]GG
HgaI 527 GCGTC GACGC(5/10)
KasI 271 GGCGCC G^GCGCC
KspI 339 CCGCGG CCGC^GG
MaeII 215 ACGT A^CGT
MflI 368 AGATCT [GA]^GATC[CT]
MluNI 657 TGGCCA TGG^CCA
MscI 657 TGGCCA TGG^CCA
MslI 501 CACATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 17 GCCGGC GCC^GGC
NarI 271 GGCGCC GG^CGCC
NciI 522 CCGGG CC^[GC]GG
NgoMIV 17 GCCGGC G^CCGGC
PflFI 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 214 CACGTG CAC^GTG
PmlI 214 CACGTG CAC^GTG
PpuMI 662 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 66 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 235 CTGCAG CTGCA^G
PvuII 479 CAGCTG CAG^CTG
SacI 637 GAGCTC GAGCT^C
SacII 339 CCGCGG CCGC^GG
SapI 315 GCTCTTC GCTCTTC(1/4)
SfcI 235 CTGCAG C^T[GA][CT]AG
SfoI 271 GGCGCC GGC^GCC
SmlI 634 CTTGAG C^T[CT][GA]AG
StyI 329 CCTTGG C^C[AT][AT]GG
Tth111I 562 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 159 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 368 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 271 GGCGCC G[GA]^CG[CT]C
526 GGCGTC
AvaI 348 CTCGGG C^[CT]CG[GA]G
620 CTCGGG
AvaII 156 GGACC G^G[AT]CC
663 GGACC
BanII 333 GGGCTC G[GA]GC[CT]^C
637 GAGCTC
BsaHI 271 GGCGCC G[GA]^CG[CT]C
526 GGCGTC
BsgI 202 CTGCAC GTGCAG(16/14)
286 CTGCAC
BsoBI 348 CTCGGG C^[CT]CG[GA]G
620 CTCGGG
BsrI 359 ACTGG ACTGG(1/-1)
402 CCAGT
DpnII 183 GATC ^GATC
369 GATC
DraII 623 GGGGCCT [GA]G^G[ACGT]CC[CT]
662 AGGACCC
DsaI 11 CCGTGG C^C[GA][CT]GG
339 CCGCGG
EcoO109I 623 GGGGCCT [GA]G^G[ACGT]CC[CT]
662 AGGACCC
FauI 338 CCCGC CCCGC(4/6)
256 GCGGG
HaeII 271 GGCGCC [GA]GCGC^[CT]
353 GGCGCT
Hin1I 271 GGCGCC G[GA]^CG[CT]C
526 GGCGTC
MboI 183 GATC ^GATC
369 GATC
NdeII 183 GATC ^GATC
369 GATC
NlaIII 276 CATG CATG^
503 CATG
Sau3AI 183 GATC ^GATC
369 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbsI BbvI BmyI BpuAI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIV RsaI Sau96I ScrFI TseI
TspRI