Restriction map for
pFN21AE1362
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DsaI Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NlaIII NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 361 GGCGCC G[GA]^CG[CT]C
Aor51HI 398 AGCGCT AGC^GCT
ApaI 500 GGGCCC GGGCC^C
AspHI 877 GAGCTC G[AT]GC[AT]^C
BbeI 361 GGCGCC GGCGC^C
BfaI 418 CTAG C^TAG
BglI 429 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 361 GGCGCC G[GA]^CG[CT]C
BsaI 679 GGTCTC GGTCTC(1/5)
BsiHKAI 877 GAGCTC G[AT]GC[AT]^C
BsmAI 680 GTCTC GTCTC(1/5)
Bsp1407I 122 TGTACA T^GTACA
BspMI 591 ACCTGC ACCTGC(4/8)
BsrGI 122 TGTACA T^GTACA
BstAPI 327 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 188 AGATCC [GA]^GATC[CT]
DraIII 328 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 25 GAAGAG CTCTTC(1/4)
Eco47III 398 AGCGCT AGC^GCT
EcoT14I 143 CCTTGG C^C[AT][AT]GG
HgaI 376 GACGC GACGC(5/10)
Hin1I 361 GGCGCC G[GA]^CG[CT]C
KasI 361 GGCGCC G^GCGCC
Ksp632I 25 GAAGAG CTCTTC(1/4)
MaeI 418 CTAG C^TAG
MflI 188 AGATCC [GA]^GATC[CT]
NaeI 358 GCCGGC GCC^GGC
NarI 361 GGCGCC GG^CGCC
NgoMIV 358 GCCGGC G^CCGGC
NotI 310 GCGGCCGC GC^GGCCGC
PaeR7I 865 CTCGAG C^TCGAG
PpuMI 734 GGGACCC [GA]G^G[AT]CC[CT]
SacI 877 GAGCTC GAGCT^C
SfoI 361 GGCGCC GGC^GCC
SgrAI 357 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 503 CCCGGG CCC^GGG
SmlI 865 CTCGAG C^T[CT][GA]AG
SspBI 122 TGTACA T^GTACA
StuI 791 AGGCCT AGG^CCT
StyI 143 CCTTGG C^C[AT][AT]GG
TaqI 866 TCGA T^CGA
TthHB8I 866 TCGA T^CGA
XcmI 920 CCAATGGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 865 CTCGAG C^TCGAG
XhoII 188 AGATCC [GA]^GATC[CT]
XmaCI 503 CCCGGG C^CCGGG
XmaI 503 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 189 GATCC GGATC(4/5)
916 GATCC
BlpI 457 GCTGAGC GC^T[ACGT]AGC
639 GCTGAGC
BpmI 346 CTGGAG CTGGAG(16/14)
463 CTGGAG
Bpu1102I 457 GCTGAGC GC^T[ACGT]AGC
639 GCTGAGC
BsgI 217 GTGCAG GTGCAG(16/14)
817 GTGCAG
BsmFI 734 GGGAC GGGAC(10/14)
777 GGGAC
BsrBI 153 GAGCGG CCGCTC(-3/-3)
367 GAGCGG
BsrFI 358 GCCGGC [GA]^CCGG[CT]
434 ACCGGC
BsrI 345 ACTGG ACTGG(1/-1)
453 ACTGG
CelII 457 GCTGAGC GC^T[ACGT]AGC
639 GCTGAGC
Cfr10I 358 GCCGGC [GA]^CCGG[CT]
434 ACCGGC
DdeI 458 CTGAG C^T[ACGT]AG
640 CTGAG
DraII 499 GGGGCCC [GA]G^G[ACGT]CC[CT]
734 GGGACCC
DrdI 70 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
514 GACGGGGAGGTC
EagI 311 CGGCCG C^GGCCG
634 CGGCCG
EclXI 311 CGGCCG C^GGCCG
634 CGGCCG
Eco52I 311 CGGCCG C^GGCCG
634 CGGCCG
EcoNI 852 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
888 CCTGGCAGAGG
EcoO109I 499 GGGGCCC [GA]G^G[ACGT]CC[CT]
734 GGGACCC
HphI 567 GGTGA GGTGA(8/7)
881 TCACC
MaeII 170 ACGT A^CGT
661 ACGT
PleI 748 GACTC GAGTC(4/5)
58 GAGTC
TfiI 227 GATTC G^A[AT]TC
630 GAATC
TspRI 119 CAGTG CA[GC]TG[ACGT][ACGT]^
707 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BsaJI BseRI BsiEI
BsiYI BslI BsoBI Bsp1286I BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI