##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsgI        BsmBI       Bsp1407I    BspDI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     CelII       ClaI        DraI        DraIII      
DrdI        DsaI        Eam1105I    Eco47III    EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MscI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tsp509I     Tth111I     XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII         291  CGCG                 CG^CG
AccIII        483  TCCGGA               T^CCGGA
AlwI          473  GGATC                GGATC(4/5)
ApaI          817  GGGCCC               GGGCC^C
BanI          546  GGTGCC               G^G[CT][GA]CC
BsaWI         483  TCCGGA               [AT]^CCGG[AT]
BseAI         483  TCCGGA               T^CCGGA
BsiEI         336  CGGCCG               CG[GA][CT]^CG
BsiWI         461  CGTACG               C^GTACG
BsmFI         825  GGGAC                GGGAC(10/14)
BsmI          163  GAATGC               GAATGC(1/-1)
BspEI         483  TCCGGA               T^CCGGA
BspHI         137  TCATGA               T^CATGA
BspMI         503  ACCTGC               ACCTGC(4/8)
BsrBI          36  GAGCGG               CCGCTC(-3/-3)
BsrDI         534  CATTGC               GCAATG(2/0)
BsrFI         488  ACCGGC               [GA]^CCGG[CT]
BstUI         291  CGCG                 CG^CG
Cfr10I        488  ACCGGC               [GA]^CCGG[CT]
CpoI          485  CGGACCG              CG^G[AT]CCG
EagI          336  CGGCCG               C^GGCCG
EarI          397  CTCTTC               CTCTTC(1/4)
EclXI         336  CGGCCG               C^GGCCG
Eco52I        336  CGGCCG               C^GGCCG
Ksp632I       397  CTCTTC               CTCTTC(1/4)
MaeII         441  ACGT                 A^CGT
MroI          483  TCCGGA               T^CCGGA
MseI          635  TTAA                 T^TAA
MslI          187  CACAACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          291  CGCG                 CG^CG
PpuMI         125  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII          94  CAGCTG               CAG^CTG
RcaI          137  TCATGA               T^CATGA
RsrII         485  CGGACCG              CG^G[AT]CCG
SfiI          267  GGCCAAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI          771  AGGCCT               AGG^CCT
Tru9I         635  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AcyI          919  GGCGTC               G[GA]^CG[CT]C
              940  GGCGTC              
BbvCI        1267  CCTCAGC              CCTCAGC(-5/-2)
             1332  GCTGAGG             
BcgI          528  CGAGACCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              554  GCAGAGAGATCG        
BsaHI         919  GGCGTC               G[GA]^CG[CT]C
              940  GGCGTC              
BstAPI        167  GCAAACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              498  GCAGTACCTGC         
EcoT14I       260  CCAAGG               C^C[AT][AT]GG
              269  CCAAGG              
HaeII          83  AGCGCC               [GA]GCGC^[CT]
              491  GGCGCT              
HgaI          920  GCGTC                GACGC(5/10)
              941  GCGTC               
Hin1I         919  GGCGTC               G[GA]^CG[CT]C
              940  GGCGTC              
StyI          260  CCAAGG               C^C[AT][AT]GG
              269  CCAAGG              
TspRI         592  CACTG                CA[GC]TG[ACGT][ACGT]^
              875  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwNI       
AocI        ApaLI       AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        Eco81I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        TaqI        TseI        Tsp45I      TthHB8I     
Van91I      XmaCI       XmaI