Restriction map for
pFN21AE6321
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
KasI Ksp632I KspI MaeII MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 638 GGTACC G^GTACC
AfaI 639 GTAC GT^AC
AflII 37 CTTAAG C^TTAAG
Asp700I 105 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 638 GGTACC G^GTACC
BbvCI 919 GCTGAGG CCTCAGC(-5/-2)
BfaI 511 CTAG C^TAG
BfrI 37 CTTAAG C^TTAAG
BsgI 827 GTGCAG GTGCAG(16/14)
BspHI 191 TCATGA T^CATGA
BssSI 530 CACGAG CACGAG(-5/-1)
BstAPI 469 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 815 CCATGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 977 AGATCC [GA]^GATC[CT]
DraII 786 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 786 AGGCCCC [GA]G^G[ACGT]CC[CT]
HincII 628 GTTGAC GT[CT]^[GA]AC
HindII 628 GTTGAC GT[CT]^[GA]AC
HindIII 172 AAGCTT A^AGCTT
KpnI 638 GGTACC GGTAC^C
MaeI 511 CTAG C^TAG
MboII 930 GAAGA GAAGA(8/7)
MfeI 633 CAATTG C^AATTG
MflI 977 AGATCC [GA]^GATC[CT]
MseI 38 TTAA T^TAA
MunI 633 CAATTG C^AATTG
NdeI 875 CATATG CA^TATG
NspI 729 GCATGT [GA]CATG^[CT]
RcaI 191 TCATGA T^CATGA
RsaI 639 GTAC GT^AC
SacI 151 GAGCTC GAGCT^C
SpeI 510 ACTAGT A^CTAGT
Tru9I 38 TTAA T^TAA
XcmI 341 CCATTGCCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 977 AGATCC [GA]^GATC[CT]
XmnI 105 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 978 GATCC GGATC(4/5)
279 GGATC
AspHI 151 GAGCTC G[AT]GC[AT]^C
439 GTGCTC
BpmI 836 CTGGAG CTGGAG(16/14)
949 CTGGAG
BsiHKAI 151 GAGCTC G[AT]GC[AT]^C
439 GTGCTC
BspMI 493 ACCTGC ACCTGC(4/8)
321 GCAGGT
BsrDI 342 CATTGC GCAATG(2/0)
799 GCAATG
BsrI 451 ACTGG ACTGG(1/-1)
835 ACTGG
EcoNI 303 CCTTGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
700 CCTCAGTTAGG
MaeIII 182 GTCAC ^GT[ACGT]AC
646 GTCAC
PleI 880 GAGTC GAGTC(4/5)
913 GAGTC
PstI 495 CTGCAG CTGCA^G
991 CTGCAG
SfcI 495 CTGCAG C^T[GA][CT]AG
991 CTGCAG
Tsp45I 182 GTCAC ^GT[GC]AC
646 GTCAC
Tsp509I 521 AATT ^AATT
634 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MboI MnlI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI SmlI TfiI TseI
TspRI