##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaJI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmeI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AfaI          190  GTAC                 GT^AC
AluI          392  AGCT                 AG^CT
ApaI          374  GGGCCC               GGGCC^C
AseI          207  ATTAAT               AT^TAAT
AsnI          207  ATTAAT               AT^TAAT
AvaII         412  GGTCC                G^G[AT]CC
BanII         374  GGGCCC               G[GA]GC[CT]^C
BbrPI          58  CACGTG               CAC^GTG
BglI          367  GCCGACGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          374  GGGCCC               G[AGT]GC[ACT]^C
BsaAI          58  CACGTG               [CT]AC^GT[GA]
BsaWI         386  ACCGGA               [AT]^CCGG[AT]
BsgI           10  GTGCAG               GTGCAG(16/14)
Bsp1286I      374  GGGCCC               G[AGT]GC[ACT]^C
BspDI         203  ATCGAT               AT^CGAT
Cac8I          75  GCTGGC               GC[ACGT]^[ACGT]GC
ClaI          203  ATCGAT               AT^CGAT
DdeI          193  CTCAG                C^T[ACGT]AG
DpnII         202  GATC                 ^GATC
HapII         387  CCGG                 C^CGG
HindIII       391  AAGCTT               A^AGCTT
HpaII         387  CCGG                 C^CGG
HphI          158  TCACC                GGTGA(8/7)
MboI          202  GATC                 ^GATC
MboII          63  GAAGA                GAAGA(8/7)
MnlI          329  GAGG                 CCTC(7/6)
MseI          208  TTAA                 T^TAA
MspA1I        408  CAGCGG               C[AC]G^C[GT]G
MspI          387  CCGG                 C^CGG
NdeII         202  GATC                 ^GATC
PmaCI          58  CACGTG               CAC^GTG
PmlI           58  CACGTG               CAC^GTG
PshBI         207  ATTAAT               AT^TAAT
RsaI          190  GTAC                 GT^AC
Sau3AI        202  GATC                 ^GATC
SfaNI          66  GATGC                GCATC(5/9)
TaqI          204  TCGA                 T^CGA
Tru9I         208  TTAA                 T^TAA
TthHB8I       204  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AciI           72  GCGG                 CCGC(-3/-1)
              410  GCGG                
BbvI           12  GCAGC                GCAGC(8/12)
               69  GCTGC               
BsrI          145  ACTGG                ACTGG(1/-1)
              252  ACTGG               
DraII         373  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              374  GGGCCCT             
EcoO109I      373  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              374  GGGCCCT             
MaeII           8  ACGT                 A^CGT
               59  ACGT                
MwoI           32  GCCCCTTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              367  GCCGACGGGGC         
NlaIII        270  CATG                 CATG^
              436  CATG                
NlaIV         373  GGGGCC               GG[ACGT]^[ACGT]CC
              374  GGGCCC              
TseI           12  GCAGC                G^C[AT]GC
               69  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

Cfr13I      Fnu4HI      HaeIII      ItaI        MaeIII      
Sau96I      Tsp45I      TspRI