Restriction map for
pFN21AE1118
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# NO CUT ENZYMES ( 142 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BcgI BciVI
BcnI BfaI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I CpoI
DraI DraIII EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MaeII MamI MfeI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 598 AAATTC [GA]^AATT[CT]
AcyI 310 GACGCC G[GA]^CG[CT]C
AflIII 811 ACGCGT A^C[GA][CT]GT
AhdI 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 615 GTGCAC G^TGCAC
ApaLI 615 GTGCAC G^TGCAC
ApoI 598 AAATTC [GA]^AATT[CT]
AspEI 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 470 GGATCC G^GATCC
BanII 232 GAGCTC G[GA]GC[CT]^C
BbvCI 906 CCTCAGC CCTCAGC(-5/-2)
BclI 917 TGATCA T^GATCA
BglI 962 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 957 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 957 GCTGAGC GC^T[ACGT]AGC
BsaHI 310 GACGCC G[GA]^CG[CT]C
BspDI 133 ATCGAT AT^CGAT
BspMI 635 ACCTGC ACCTGC(4/8)
CelII 957 GCTGAGC GC^T[ACGT]AGC
CfoI 621 GCGC GCG^C
ClaI 133 ATCGAT AT^CGAT
DrdI 219 GACCTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 708 CCATGG C^C[GA][CT]GG
Eam1105I 412 GACTATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 917 TGATCA T^GATCA
HhaI 621 GCGC GCG^C
Hin1I 310 GACGCC G[GA]^CG[CT]C
HinP1I 621 GCGC G^CGC
MluI 811 ACGCGT A^CGCGT
MseI 862 TTAA T^TAA
NcoI 708 CCATGG C^CATGG
PleI 782 GACTC GAGTC(4/5)
PstI 487 CTGCAG CTGCA^G
PvuII 379 CAGCTG CAG^CTG
SacI 232 GAGCTC GAGCT^C
SapI 45 GAAGAGC GCTCTTC(1/4)
SbfI 486 CCTGCAGG CCTGCA^GG
SfcI 487 CTGCAG C^T[GA][CT]AG
Sse8387I 486 CCTGCAGG CCTGCA^GG
TaqI 134 TCGA T^CGA
TfiI 576 GATTC G^A[AT]TC
Tru9I 862 TTAA T^TAA
TthHB8I 134 TCGA T^CGA
XcmI 728 CCATGCTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 620 CGCG CG^CG
812 CGCG
AfaI 273 GTAC GT^AC
894 GTAC
AlwNI 68 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
550 CAGGATCTG
BbsI 409 GAAGAC GAAGAC(2/6)
925 GAAGAC
BpuAI 409 GAAGAC GAAGAC(2/6)
925 GAAGAC
BseRI 430 GAGGAG GAGGAG(10/8)
721 GAGGAG
BsrI 531 ACTGG ACTGG(1/-1)
717 ACTGG
BstUI 620 CGCG CG^CG
812 CGCG
DraII 206 AGGACCT [GA]G^G[ACGT]CC[CT]
820 GGGACCC
EarI 45 GAAGAG CTCTTC(1/4)
652 GAAGAG
EcoNI 426 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
483 CCTCCTGCAGG
EcoO109I 206 AGGACCT [GA]G^G[ACGT]CC[CT]
820 GGGACCC
FauI 276 CCCGC CCCGC(4/6)
384 GCGGG
HinfI 576 GATTC G^A[ACGT]TC
782 GACTC
Ksp632I 45 GAAGAG CTCTTC(1/4)
652 GAAGAG
MvnI 620 CGCG CG^CG
812 CGCG
MwoI 162 GCAGCGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
962 GCCTGGTGGGC
PpuMI 206 AGGACCT [GA]G^G[AT]CC[CT]
820 GGGACCC
RsaI 273 GTAC GT^AC
894 GTAC
SfaNI 76 GATGC GCATC(5/9)
692 GCATC
Tsp509I 599 AATT ^AATT
666 AATT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeIII HgaI
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI StyI TseI Tsp45I TspRI
XhoII