Restriction map for
pFN21AE4437
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvrII BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BsrDI BsrFI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 74 TCCGGA T^CCGGA
AcsI 706 AAATTC [GA]^AATT[CT]
AflIII 1094 ACATGT A^C[GA][CT]GT
AhdI 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 706 AAATTC [GA]^AATT[CT]
AspEI 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 289 GAGCTC G[AT]GC[AT]^C
AviII 1034 TGCGCA TGC^GCA
BalI 720 TGGCCA TGG^CCA
BbeI 379 GGCGCC GGCGC^C
BbsI 264 GAAGAC GAAGAC(2/6)
BfaI 202 CTAG C^TAG
BglII 1001 AGATCT A^GATCT
BpuAI 264 GAAGAC GAAGAC(2/6)
BsaAI 652 TACGTG [CT]AC^GT[GA]
BsaI 514 GAGACC GGTCTC(1/5)
BsaWI 74 TCCGGA [AT]^CCGG[AT]
BseAI 74 TCCGGA T^CCGGA
BsgI 154 CTGCAC GTGCAG(16/14)
BsiHKAI 289 GAGCTC G[AT]GC[AT]^C
Bsp1407I 554 TGTACA T^GTACA
BspEI 74 TCCGGA T^CCGGA
BspLU11I 1094 ACATGT A^CATGT
BsrBI 1275 CCGCTC CCGCTC(-3/-3)
BsrGI 554 TGTACA T^GTACA
BstAPI 1191 GCACTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 55 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 973 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 55 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 32 CCCGC CCCGC(4/6)
FspI 1034 TGCGCA TGC^GCA
KasI 379 GGCGCC G^GCGCC
KspI 245 CCGCGG CCGC^GG
MaeI 202 CTAG C^TAG
MluNI 720 TGGCCA TGG^CCA
MroI 74 TCCGGA T^CCGGA
MscI 720 TGGCCA TGG^CCA
MseI 1121 TTAA T^TAA
NarI 379 GGCGCC GG^CGCC
NspI 1094 ACATGT [GA]CATG^[CT]
PpuMI 55 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 612 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 85 CAGCTG CAG^CTG
SacI 289 GAGCTC GAGCT^C
SacII 245 CCGCGG CCGC^GG
SapI 1248 GAAGAGC GCTCTTC(1/4)
SfoI 379 GGCGCC GGC^GCC
SmaI 836 CCCGGG CCC^GGG
SspBI 554 TGTACA T^GTACA
TfiI 851 GAATC G^A[AT]TC
Tru9I 1121 TTAA T^TAA
XmaCI 836 CCCGGG C^CCGGG
XmaI 836 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 267 GACGCC G[GA]^CG[CT]C
379 GGCGCC
Asp700I 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAAGAGCTTC
BamHI 77 GGATCC G^GATCC
1231 GGATCC
BsaHI 267 GACGCC G[GA]^CG[CT]C
379 GGCGCC
BspHI 587 TCATGA T^CATGA
710 TCATGA
BspMI 152 ACCTGC ACCTGC(4/8)
727 ACCTGC
BssHII 27 GCGCGC G^CGCGC
137 GCGCGC
EarI 639 GAAGAG CTCTTC(1/4)
1248 GAAGAG
EcoT14I 371 CCATGG C^C[AT][AT]GG
1239 CCATGG
HgaI 267 GACGC GACGC(5/10)
689 GACGC
Hin1I 267 GACGCC G[GA]^CG[CT]C
379 GGCGCC
Ksp632I 639 GAAGAG CTCTTC(1/4)
1248 GAAGAG
NcoI 371 CCATGG C^CATGG
1239 CCATGG
PleI 276 GACTC GAGTC(4/5)
642 GAGTC
RcaI 587 TCATGA T^CATGA
710 TCATGA
StuI 1291 AGGCCT AGG^CCT
1311 AGGCCT
StyI 371 CCATGG C^C[AT][AT]GG
1239 CCATGG
Tsp509I 707 AATT ^AATT
777 AATT
XcmI 362 CCATCCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1145 CCAGTGGTGGGGTGG
XmnI 804 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1248 GAAGAGCTTC
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoNI EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII