Restriction map for
pFN21AE5401
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BciVI BfaI BfrI BglI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BsgI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 718 GTCTAC GT^[AC][GT]AC
AflIII 773 ACGTGT A^C[GA][CT]GT
AlwNI 427 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 799 GAGCTC G[AT]GC[AT]^C
BbeI 25 GGCGCC GGCGC^C
BbvCI 419 CCTCAGC CCTCAGC(-5/-2)
BcgI 395 CGACCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 308 TGATCA T^GATCA
BlpI 468 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
BseRI 586 GAGGAG GAGGAG(10/8)
BsiHKAI 799 GAGCTC G[AT]GC[AT]^C
BsiWI 38 CGTACG C^GTACG
BsmAI 121 GAGAC GTCTC(1/5)
BsmBI 121 GAGACG CGTCTC(1/5)
BsrFI 561 GCCGGC [GA]^CCGG[CT]
BsrI 830 CCAGT ACTGG(1/-1)
CelII 468 GCTGAGC GC^T[ACGT]AGC
Cfr10I 561 GCCGGC [GA]^CCGG[CT]
DraIII 514 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 203 CGGCCG C^GGCCG
EclXI 203 CGGCCG C^GGCCG
Eco52I 203 CGGCCG C^GGCCG
FbaI 308 TGATCA T^GATCA
HphI 311 TCACC GGTGA(8/7)
KasI 25 GGCGCC G^GCGCC
MslI 312 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 136 CAGCTG C[AC]G^C[GT]G
NaeI 561 GCCGGC GCC^GGC
NarI 25 GGCGCC GG^CGCC
NcoI 743 CCATGG C^CATGG
NgoMIV 561 GCCGGC G^CCGGC
NspI 473 GCATGC [GA]CATG^[CT]
PaeR7I 583 CTCGAG C^TCGAG
PpuMI 488 GGGACCC [GA]G^G[AT]CC[CT]
PstI 757 CTGCAG CTGCA^G
PvuI 852 CGATCG CGAT^CG
PvuII 136 CAGCTG CAG^CTG
SacI 799 GAGCTC GAGCT^C
SexAI 50 ACCTGGT A^CC[AT]GGT
SfcI 757 CTGCAG C^T[GA][CT]AG
SfoI 25 GGCGCC GGC^GCC
SmaI 664 CCCGGG CCC^GGG
SmlI 583 CTCGAG C^T[CT][GA]AG
SphI 473 GCATGC GCATG^C
TaqI 584 TCGA T^CGA
TthHB8I 584 TCGA T^CGA
XcmI 638 CCACCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 583 CTCGAG C^TCGAG
XmaCI 664 CCCGGG C^CCGGG
XmaI 664 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
178 GACGCC
ApaI 692 GGGCCC GGGCC^C
870 GGGCCC
AvaI 583 CTCGAG C^[CT]CG[GA]G
664 CCCGGG
BalI 678 TGGCCA TGG^CCA
740 TGGCCA
BanI 25 GGCGCC G^G[CT][GA]CC
558 GGTGCC
BbrPI 451 CACGTG CAC^GTG
772 CACGTG
BsaHI 25 GGCGCC G[GA]^CG[CT]C
178 GACGCC
BsiEI 203 CGGCCG CG[GA][CT]^CG
852 CGATCG
BsmFI 272 GGGAC GGGAC(10/14)
488 GGGAC
BsoBI 583 CTCGAG C^[CT]CG[GA]G
664 CCCGGG
BssHII 11 GCGCGC G^CGCGC
62 GCGCGC
BstXI 641 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
743 CCATGGTGCTGG
DdeI 420 CTCAG C^T[ACGT]AG
469 CTGAG
EarI 289 CTCTTC CTCTTC(1/4)
724 CTCTTC
EcoNI 582 CCTCGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
696 CCTCTACAAGG
EcoT14I 29 CCTTGG C^C[AT][AT]GG
743 CCATGG
HaeII 25 GGCGCC [GA]GCGC^[CT]
428 AGCGCC
HgaI 123 GACGC GACGC(5/10)
178 GACGC
Hin1I 25 GGCGCC G[GA]^CG[CT]C
178 GACGCC
HinfI 447 GACTC G^A[ACGT]TC
883 GAGTC
Ksp632I 289 CTCTTC CTCTTC(1/4)
724 CTCTTC
MluNI 678 TGGCCA TGG^CCA
740 TGGCCA
MscI 678 TGGCCA TGG^CCA
740 TGGCCA
PleI 447 GACTC GAGTC(4/5)
883 GAGTC
PmaCI 451 CACGTG CAC^GTG
772 CACGTG
PmlI 451 CACGTG CAC^GTG
772 CACGTG
SfaNI 349 GATGC GCATC(5/9)
607 GATGC
StuI 359 AGGCCT AGG^CCT
590 AGGCCT
StyI 29 CCTTGG C^C[AT][AT]GG
743 CCATGG
TspRI 341 CACTG CA[GC]TG[ACGT][ACGT]^
437 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BcnI BmyI BpmI
BsaAI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
Tsp45I