Restriction map for
pFN21AE1502
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI HphI KpnI
MamI MfeI MflI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 1048 GTAC GT^AC
AflIII 774 ACGCGT A^C[GA][CT]GT
AhdI 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 685 GGATC GGATC(4/5)
Aor51HI 755 AGCGCT AGC^GCT
ApaI 419 GGGCCC GGGCC^C
AspEI 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1078 GAGCTC G[AT]GC[AT]^C
BbvCI 948 CCTCAGC CCTCAGC(-5/-2)
BfaI 193 CTAG C^TAG
BseRI 850 CTCCTC GAGGAG(10/8)
BsiHKAI 1078 GAGCTC G[AT]GC[AT]^C
BsmBI 310 GAGACG CGTCTC(1/5)
BsrBI 842 GAGCGG CCGCTC(-3/-3)
DpnII 686 GATC ^GATC
DraIII 657 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 538 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1009 GAAGAG CTCTTC(1/4)
Eco47III 755 AGCGCT AGC^GCT
EcoNI 291 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 95 CCTTGG C^C[AT][AT]GG
HinfI 865 GATTC G^A[ACGT]TC
Ksp632I 1009 GAAGAG CTCTTC(1/4)
MaeI 193 CTAG C^TAG
MaeIII 358 GTCAC ^GT[ACGT]AC
MboI 686 GATC ^GATC
MboII 1009 GAAGA GAAGA(8/7)
MluI 774 ACGCGT A^CGCGT
NaeI 340 GCCGGC GCC^GGC
NdeII 686 GATC ^GATC
NgoMIV 340 GCCGGC G^CCGGC
NheI 192 GCTAGC G^CTAGC
NotI 974 GCGGCCGC GC^GGCCGC
NruI 729 TCGCGA TCG^CGA
RsaI 1048 GTAC GT^AC
SacI 1078 GAGCTC GAGCT^C
Sau3AI 686 GATC ^GATC
SmlI 826 CTCAAG C^T[CT][GA]AG
StyI 95 CCTTGG C^C[AT][AT]GG
TfiI 865 GATTC G^A[AT]TC
Tsp45I 358 GTCAC ^GT[GC]AC
Tsp509I 256 AATT ^AATT
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AviII 698 TGCGCA TGC^GCA
1070 TGCGCA
BlpI 169 GCTGAGC GC^T[ACGT]AGC
1080 GCTCAGC
BpmI 261 CTCCAG CTGGAG(16/14)
751 CTGGAG
Bpu1102I 169 GCTGAGC GC^T[ACGT]AGC
1080 GCTCAGC
BsgI 610 CTGCAC GTGCAG(16/14)
1000 GTGCAG
BsmAI 310 GAGAC GTCTC(1/5)
587 GAGAC
BsmFI 536 GGGAC GGGAC(10/14)
579 GGGAC
BspMI 703 ACCTGC ACCTGC(4/8)
962 ACCTGC
BsrFI 340 GCCGGC [GA]^CCGG[CT]
898 ACCGGC
BssHII 692 GCGCGC G^CGCGC
740 GCGCGC
BstAPI 656 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
711 GCAGAAGCTGC
BstF5I 288 CATCC GGATG(2/0)
591 CATCC
CelII 169 GCTGAGC GC^T[ACGT]AGC
1080 GCTCAGC
Cfr10I 340 GCCGGC [GA]^CCGG[CT]
898 ACCGGC
DraII 419 GGGCCCC [GA]G^G[ACGT]CC[CT]
800 AGGCCCT
EagI 338 CGGCCG C^GGCCG
975 CGGCCG
EclXI 338 CGGCCG C^GGCCG
975 CGGCCG
Eco52I 338 CGGCCG C^GGCCG
975 CGGCCG
EcoO109I 419 GGGCCCC [GA]G^G[ACGT]CC[CT]
800 AGGCCCT
FokI 288 CATCC GGATG(9/13)
591 CATCC
FspI 698 TGCGCA TGC^GCA
1070 TGCGCA
MaeII 62 ACGT A^CGT
524 ACGT
NlaIII 689 CATG CATG^
780 CATG
PstI 120 CTGCAG CTGCA^G
388 CTGCAG
SfaNI 116 GCATC GCATC(5/9)
287 GCATC
SfcI 120 CTGCAG C^T[GA][CT]AG
388 CTGCAG
StuI 275 AGGCCT AGG^CCT
365 AGGCCT
TaqI 547 TCGA T^CGA
1007 TCGA
TthHB8I 547 TCGA T^CGA
1007 TCGA
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI KspI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV SacII
Sau96I ScrFI SfoI TseI TspRI