Restriction map for
pFN21AE4326
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BsrDI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 307 GTCTAC GT^[AC][GT]AC
AccIII 738 TCCGGA T^CCGGA
AfaI 807 GTAC GT^AC
AflIII 512 ACATGT A^C[GA][CT]GT
Alw44I 964 GTGCAC G^TGCAC
ApaLI 964 GTGCAC G^TGCAC
AspI 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 493 CACGTG CAC^GTG
BcgI 948 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 493 CACGTG [CT]AC^GT[GA]
BsaWI 738 TCCGGA [AT]^CCGG[AT]
BseAI 738 TCCGGA T^CCGGA
BseRI 646 GAGGAG GAGGAG(10/8)
BsmAI 831 GTCTC GTCTC(1/5)
Bsp1407I 806 TGTACA T^GTACA
BspEI 738 TCCGGA T^CCGGA
BspLU11I 512 ACATGT A^CATGT
BspMI 441 GCAGGT ACCTGC(4/8)
BsrGI 806 TGTACA T^GTACA
BstAPI 224 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 719 GGATCT [GA]^GATC[CT]
DsaI 194 CCGCGG C^C[GA][CT]GG
EarI 931 CTCTTC CTCTTC(1/4)
EcoT14I 636 CCTTGG C^C[AT][AT]GG
HinfI 681 GACTC G^A[ACGT]TC
HphI 633 TCACC GGTGA(8/7)
Ksp632I 931 CTCTTC CTCTTC(1/4)
KspI 194 CCGCGG CCGC^GG
MaeII 494 ACGT A^CGT
MboII 932 TCTTC GAAGA(8/7)
MflI 719 GGATCT [GA]^GATC[CT]
MroI 738 TCCGGA T^CCGGA
MslI 507 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 705 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 681 GACTC GAGTC(4/5)
PmaCI 493 CACGTG CAC^GTG
PmlI 493 CACGTG CAC^GTG
PstI 370 CTGCAG CTGCA^G
RsaI 807 GTAC GT^AC
SacI 238 GAGCTC GAGCT^C
SacII 194 CCGCGG CCGC^GG
SbfI 369 CCTGCAGG CCTGCA^GG
SexAI 704 ACCAGGT A^CC[AT]GGT
SfcI 370 CTGCAG C^T[GA][CT]AG
SfiI 201 GGCCGCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 351 CCCGGG CCC^GGG
SmlI 550 CTCAAG C^T[CT][GA]AG
SphI 224 GCATGC GCATG^C
Sse8387I 369 CCTGCAGG CCTGCA^GG
SspBI 806 TGTACA T^GTACA
StuI 477 AGGCCT AGG^CCT
StyI 636 CCTTGG C^C[AT][AT]GG
Tth111I 703 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 705 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 506 CCATGAACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 719 GGATCT [GA]^GATC[CT]
XmaCI 351 CCCGGG C^CCGGG
XmaI 351 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 888 GATCC GGATC(4/5)
719 GGATC
ApaI 169 GGGCCC GGGCC^C
750 GGGCCC
BanI 217 GGCGCC G^G[CT][GA]CC
427 GGCGCC
BbeI 217 GGCGCC GGCGC^C
427 GGCGCC
BglI 193 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
202 GCCGCAGTGGC
BsgI 141 CTGCAC GTGCAG(16/14)
775 CTGCAC
BsmFI 413 GGGAC GGGAC(10/14)
914 GGGAC
BsrBI 715 GAGCGG CCGCTC(-3/-3)
745 GAGCGG
BsrFI 177 GCCGGT [GA]^CCGG[CT]
605 ACCGGC
BsrI 81 ACTGG ACTGG(1/-1)
699 ACTGG
BssHII 109 GCGCGC G^CGCGC
111 GCGCGC
Cfr10I 177 GCCGGT [GA]^CCGG[CT]
605 ACCGGC
DdeI 468 CTCAG C^T[ACGT]AG
596 CTGAG
DpnII 720 GATC ^GATC
888 GATC
HgaI 415 GACGC GACGC(5/10)
305 GCGTC
KasI 217 GGCGCC G^GCGCC
427 GGCGCC
MaeIII 592 GTCAC ^GT[ACGT]AC
791 GTGAC
MboI 720 GATC ^GATC
888 GATC
NarI 217 GGCGCC GG^CGCC
427 GGCGCC
NdeII 720 GATC ^GATC
888 GATC
NspI 224 GCATGC [GA]CATG^[CT]
512 ACATGT
PpuMI 707 AGGTCCT [GA]G^G[AT]CC[CT]
914 GGGACCC
Sau3AI 720 GATC ^GATC
888 GATC
SfaNI 978 GATGC GCATC(5/9)
630 GCATC
SfoI 217 GGCGCC GGC^GCC
427 GGCGCC
Tsp45I 592 GTCAC ^GT[GC]AC
791 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI TspRI