Restriction map for
pFN21AE4334
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsaI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI BsoBI BspDI
BspHI BsrBI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI DsaI Eco47III
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MscI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
TfiI Tru9I Tsp509I Tth111I TthHB8I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 937 TCCGGA T^CCGGA
AcyI 250 GGCGCC G[GA]^CG[CT]C
AhdI 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 940 GGATC GGATC(4/5)
AocI 908 CCTCAGG CC^T[ACGT]AGG
AspEI 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 250 GGCGCC GGCGC^C
BfaI 1024 CTAG C^TAG
BglII 685 AGATCT A^GATCT
BlpI 180 GCTGAGC GC^T[ACGT]AGC
BpmI 642 CTCCAG CTGGAG(16/14)
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
BsaHI 250 GGCGCC G[GA]^CG[CT]C
BsaWI 937 TCCGGA [AT]^CCGG[AT]
BseAI 937 TCCGGA T^CCGGA
Bsp1407I 134 TGTACA T^GTACA
BspEI 937 TCCGGA T^CCGGA
BspLU11I 532 ACATGT A^CATGT
BspMI 670 ACCTGC ACCTGC(4/8)
BsrDI 419 GCAATG GCAATG(2/0)
BsrGI 134 TGTACA T^GTACA
BssSI 189 CTCGTG CACGAG(-5/-1)
Bsu36I 908 CCTCAGG CC^T[ACGT]AGG
CelII 180 GCTGAGC GC^T[ACGT]AGC
DraII 128 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 186 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 826 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 908 CCTCAGG CC^T[ACGT]AGG
EcoNI 657 CCTGAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 128 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 739 GATATC GAT^ATC
EcoT14I 480 CCAAGG C^C[AT][AT]GG
HaeII 250 GGCGCC [GA]GCGC^[CT]
HgaI 347 GCGTC GACGC(5/10)
Hin1I 250 GGCGCC G[GA]^CG[CT]C
HinfI 632 GAGTC G^A[ACGT]TC
KasI 250 GGCGCC G^GCGCC
MaeI 1024 CTAG C^TAG
MroI 937 TCCGGA T^CCGGA
MslI 531 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 176 GCCGGC GCC^GGC
NarI 250 GGCGCC GG^CGCC
NgoMIV 176 GCCGGC G^CCGGC
NspI 532 ACATGT [GA]CATG^[CT]
PflMI 57 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 632 GAGTC GAGTC(4/5)
PpuMI 128 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 165 CTGCAG CTGCA^G
SfaNI 263 GCATC GCATC(5/9)
SfoI 250 GGCGCC GGC^GCC
SspBI 134 TGTACA T^GTACA
StyI 480 CCAAGG C^C[AT][AT]GG
Van91I 57 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1023 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 7 CGCG CG^CG
9 CGCG
AfaI 135 GTAC GT^AC
477 GTAC
AflIII 280 ACGTGT A^C[GA][CT]GT
532 ACATGT
Alw44I 428 GTGCAC G^TGCAC
747 GTGCAC
ApaLI 428 GTGCAC G^TGCAC
747 GTGCAC
BanI 14 GGCACC G^G[CT][GA]CC
250 GGCGCC
BbsI 961 GAAGAC GAAGAC(2/6)
988 GAAGAC
BcnI 22 CCGGG CC^[GC]GG
357 CCCGG
BglI 21 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
674 GCCTGCTGGGC
BpuAI 961 GAAGAC GAAGAC(2/6)
988 GAAGAC
BsiEI 117 CGGCCG CG[GA][CT]^CG
174 CGGCCG
BstUI 7 CGCG CG^CG
9 CGCG
BstYI 685 AGATCT [GA]^GATC[CT]
940 GGATCT
DpnII 686 GATC ^GATC
941 GATC
EaeI 117 CGGCCG [CT]^GGCC[GA]
174 CGGCCG
EagI 117 CGGCCG C^GGCCG
174 CGGCCG
EarI 223 CTCTTC CTCTTC(1/4)
973 GAAGAG
EclXI 117 CGGCCG C^GGCCG
174 CGGCCG
Eco52I 117 CGGCCG C^GGCCG
174 CGGCCG
FauI 341 CCCGC CCCGC(4/6)
395 GCGGG
HphI 374 TCACC GGTGA(8/7)
635 TCACC
Ksp632I 223 CTCTTC CTCTTC(1/4)
973 GAAGAG
MboI 686 GATC ^GATC
941 GATC
MflI 685 AGATCT [GA]^GATC[CT]
940 GGATCT
MspA1I 120 CCGCTG C[AC]G^C[GT]G
342 CCGCTG
MvnI 7 CGCG CG^CG
9 CGCG
NciI 22 CCGGG CC^[GC]GG
357 CCCGG
NdeII 686 GATC ^GATC
941 GATC
RsaI 135 GTAC GT^AC
477 GTAC
Sau3AI 686 GATC ^GATC
941 GATC
SfcI 165 CTGCAG C^T[GA][CT]AG
999 CTACAG
XcmI 530 CCACATGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
744 CCAGTGCACTGGTGG
XhoII 685 AGATCT [GA]^GATC[CT]
940 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MaeIII
MboII MnlI MspI MvaI MwoI
NlaIII NlaIV Sau96I ScrFI TseI
Tsp45I TspRI