Restriction map for
pFN21AE5114
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# NO CUT ENZYMES ( 204 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstYI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeII MamI MflI MluI
MroI MseI MslI MspI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I TseI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Asp700I 16 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 217 TGGCCA TGG^CCA
BfaI 47 CTAG C^TAG
BsaJI 257 CCTGGG C^C[ACGT][ACGT]GG
BseRI 107 CTCCTC GAGGAG(10/8)
BsmAI 105 GTCTC GTCTC(1/5)
BsmFI 200 GGGAC GGGAC(10/14)
BspDI 230 ATCGAT AT^CGAT
BssKI 257 CCTGG ^CC[ACGT]GG
BstNI 257 CCTGG CC^[AT]GG
BstXI 303 CCAGCAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 230 ATCGAT AT^CGAT
DdeI 101 CTGAG C^T[ACGT]AG
EaeI 217 TGGCCA [CT]^GGCC[GA]
EarI 34 GAAGAG CTCTTC(1/4)
EcoRII 257 CCTGG ^CC[AT]GG
FauI 273 CCCGC CCCGC(4/6)
Fnu4HI 333 GCGGC GC^[ACGT]GC
ItaI 333 GCGGC GC^[ACGT]GC
Ksp632I 34 GAAGAG CTCTTC(1/4)
MaeI 47 CTAG C^TAG
MboII 34 GAAGA GAAGA(8/7)
MfeI 113 CAATTG C^AATTG
MluNI 217 TGGCCA TGG^CCA
MscI 217 TGGCCA TGG^CCA
MspA1I 331 CAGCGG C[AC]G^C[GT]G
MunI 113 CAATTG C^AATTG
MvaI 257 CCTGG CC^[AT]GG
NlaIII 247 CATG CATG^
NlaIV 146 GGAACC GG[ACGT]^[ACGT]CC
PflMI 236 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 103 GAGTC GAGTC(4/5)
ScrFI 257 CCTGG CC^[ACGT]GG
TaqI 231 TCGA T^CGA
TfiI 53 GATTC G^A[AT]TC
Tsp45I 224 GTGAC ^GT[GC]AC
Tsp509I 114 AATT ^AATT
TthHB8I 231 TCGA T^CGA
Van91I 236 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 16 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 274 CCGC CCGC(-3/-1)
333 GCGG
AluI 18 AGCT AG^CT
138 AGCT
AlwI 233 GATCC GGATC(4/5)
254 GATCC
BsiYI 236 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
337 CCTCCTTACGG
BslI 236 CCAATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
337 CCTCCTTACGG
DpnII 233 GATC ^GATC
254 GATC
HaeIII 218 GGCC GG^CC
335 GGCC
HinfI 53 GATTC G^A[ACGT]TC
103 GAGTC
MboI 233 GATC ^GATC
254 GATC
NdeII 233 GATC ^GATC
254 GATC
Sau3AI 233 GATC ^GATC
254 GATC
TspRI 9 CACTG CA[GC]TG[ACGT][ACGT]^
241 CAGTG
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# 3 OR MORE CUT ENZYMES ( 2 enzymes) #
#########################################
MaeIII MnlI