Restriction map for
pFN21AE3609
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# NO CUT ENZYMES ( 167 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRII EcoRV FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MspA1I MspI MvaI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 3 GACGTC GACGT^C
AcyI 3 GACGTC G[GA]^CG[CT]C
AhdI 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 674 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 395 GGTCC G^G[AT]CC
BalI 966 TGGCCA TGG^CCA
BbsI 124 GAAGAC GAAGAC(2/6)
BcgI 174 CGAATATCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 956 GTATCC GTATCC(6/5)
BfaI 692 CTAG C^TAG
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaHI 3 GACGTC G[GA]^CG[CT]C
BsaI 930 GGTCTC GGTCTC(1/5)
BsaJI 795 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 111 CCAAATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 111 CCAAATCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 396 GTCCC GGGAC(10/14)
BstYI 290 AGATCC [GA]^GATC[CT]
Cfr13I 395 GGTCC G^G[ACGT]CC
DraII 394 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 966 TGGCCA [CT]^GGCC[GA]
Eam1105I 388 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 297 GAAGAG CTCTTC(1/4)
EcoO109I 394 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 201 GAATTC G^AATTC
EcoT14I 795 CCAAGG C^C[AT][AT]GG
HaeIII 967 GGCC GG^CC
Hin1I 3 GACGTC G[GA]^CG[CT]C
Ksp632I 297 GAAGAG CTCTTC(1/4)
MaeI 692 CTAG C^TAG
MaeII 4 ACGT A^CGT
MfeI 423 CAATTG C^AATTG
MflI 290 AGATCC [GA]^GATC[CT]
MluNI 966 TGGCCA TGG^CCA
MscI 966 TGGCCA TGG^CCA
MunI 423 CAATTG C^AATTG
NlaIV 395 GGTCCC GG[ACGT]^[ACGT]CC
NspI 373 GCATGC [GA]CATG^[CT]
PflMI 111 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 529 GAGTC GAGTC(4/5)
PpuMI 394 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau96I 395 GGTCC G^G[ACGT]CC
SphI 373 GCATGC GCATG^C
SspI 19 AATATT AAT^ATT
StyI 795 CCAAGG C^C[AT][AT]GG
TaqI 173 TCGA T^CGA
TthHB8I 173 TCGA T^CGA
Van91I 111 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 290 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
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AlwI 291 GATCC GGATC(4/5)
855 GGATC
Asp700I 16 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
159 GAAAAAATTC
BpmI 454 CTGGAG CTGGAG(16/14)
893 CTGGAG
BsgI 593 CTGCAC GTGCAG(16/14)
1005 CTGCAC
DdeI 218 CTCAG C^T[ACGT]AG
1016 CTGAG
MaeIII 386 GTGAC ^GT[ACGT]AC
835 GTCAC
SfcI 62 CTGTAG C^T[GA][CT]AG
821 CTACAG
Tsp45I 386 GTGAC ^GT[GC]AC
835 GTCAC
XmnI 16 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
159 GAAAAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsmAI
BsrI BstF5I Cac8I DpnII EcoT22I
Fnu4HI FokI HinfI HphI ItaI
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NsiI Sau3AI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI