Restriction map for
pFN21AB6150
##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AlwNI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII BalI BanI BbeI
BbrPI BcgI BfrI BglI BglII
BsaI BseAI BsiEI BsiWI BsmFI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CfoI
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HaeII
HgaI HhaI HinP1I HpaI KasI
KpnI KspI MluI MluNI MroI
MscI MslI NarI NcoI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
AatII 1804 GACGTC GACGT^C
AccII 11 CGCG CG^CG
AcyI 1804 GACGTC G[GA]^CG[CT]C
AflIII 198 ACATGT A^C[GA][CT]GT
AgeI 115 ACCGGT A^CCGGT
Alw44I 1295 GTGCAC G^TGCAC
AocI 1080 CCTCAGG CC^T[ACGT]AGG
ApaI 1663 GGGCCC GGGCC^C
ApaLI 1295 GTGCAC G^TGCAC
AvaI 23 CCCGGG C^[CT]CG[GA]G
AvrII 1611 CCTAGG C^CTAGG
BamHI 1302 GGATCC G^GATCC
BbsI 1606 GAAGAC GAAGAC(2/6)
BbvCI 34 GCTGAGG CCTCAGC(-5/-2)
BciVI 147 GGATAC GTATCC(6/5)
BlnI 1611 CCTAGG C^CTAGG
BpuAI 1606 GAAGAC GAAGAC(2/6)
BsaAI 1874 CACGTA [CT]AC^GT[GA]
BsaHI 1804 GACGTC G[GA]^CG[CT]C
BsaWI 115 ACCGGT [AT]^CCGG[AT]
BseRI 94 CTCCTC GAGGAG(10/8)
BsgI 1236 GTGCAG GTGCAG(16/14)
BsmBI 1489 GAGACG CGTCTC(1/5)
BsoBI 23 CCCGGG C^[CT]CG[GA]G
Bsp1407I 517 TGTACA T^GTACA
BspHI 287 TCATGA T^CATGA
BspLU11I 198 ACATGT A^CATGT
BspMI 930 GCAGGT ACCTGC(4/8)
BsrGI 517 TGTACA T^GTACA
BssSI 106 CTCGTG CACGAG(-5/-1)
BstUI 11 CGCG CG^CG
BstXI 582 CCAGTTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1302 GGATCC [GA]^GATC[CT]
Bsu36I 1080 CCTCAGG CC^T[ACGT]AGG
DraI 775 TTTAAA TTT^AAA
DraII 1662 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 44 TGGCCG [CT]^GGCC[GA]
Eco81I 1080 CCTCAGG CC^T[ACGT]AGG
EcoNI 1611 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1662 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1123 GATATC GAT^ATC
Hin1I 1804 GACGTC G[GA]^CG[CT]C
HincII 798 GTTGAC GT[CT]^[GA]AC
HindII 798 GTTGAC GT[CT]^[GA]AC
MfeI 1317 CAATTG C^AATTG
MflI 1302 GGATCC [GA]^GATC[CT]
MunI 1317 CAATTG C^AATTG
MvnI 11 CGCG CG^CG
NaeI 1544 GCCGGC GCC^GGC
NdeI 1026 CATATG CA^TATG
NgoMIV 1544 GCCGGC G^CCGGC
NspI 198 ACATGT [GA]CATG^[CT]
PinAI 115 ACCGGT A^CCGGT
PstI 241 CTGCAG CTGCA^G
RcaI 287 TCATGA T^CATGA
SapI 1891 GAAGAGC GCTCTTC(1/4)
ScaI 569 AGTACT AGT^ACT
SfcI 241 CTGCAG C^T[GA][CT]AG
SmaI 23 CCCGGG CCC^GGG
SspBI 517 TGTACA T^GTACA
StuI 915 AGGCCT AGG^CCT
XbaI 936 TCTAGA T^CTAGA
XcmI 1440 CCAAATTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1302 GGATCC [GA]^GATC[CT]
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BclI 606 TGATCA T^GATCA
1586 TGATCA
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
1720 GCTCAGC
BpmI 833 CTCCAG CTGGAG(16/14)
1567 CTCCAG
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
1720 GCTCAGC
BsaBI 601 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1587 GATCAACATC
BsmAI 979 GAGAC GTCTC(1/5)
1489 GAGAC
BsmI 339 GAATGC GAATGC(1/-1)
882 GAATGC
BsrDI 550 CATTGC GCAATG(2/0)
1051 GCAATG
BsrFI 115 ACCGGT [GA]^CCGG[CT]
1544 GCCGGC
CelII 1018 GCTGAGC GC^T[ACGT]AGC
1720 GCTCAGC
Cfr10I 115 ACCGGT [GA]^CCGG[CT]
1544 GCCGGC
EcoT14I 227 CCAAGG C^C[AT][AT]GG
1611 CCTAGG
EcoT22I 884 ATGCAT ATGCA^T
1087 ATGCAT
FauI 12 GCGGG CCCGC(4/6)
73 GCGGG
FbaI 606 TGATCA T^GATCA
1586 TGATCA
HindIII 769 AAGCTT A^AGCTT
1279 AAGCTT
MamI 601 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1587 GATCAACATC
MspA1I 392 CAGCTG C[AC]G^C[GT]G
422 CAGCTG
NsiI 884 ATGCAT ATGCA^T
1087 ATGCAT
PvuII 392 CAGCTG CAG^CTG
422 CAGCTG
SmlI 97 CTCAAG C^T[CT][GA]AG
1742 CTCAAG
StyI 227 CCAAGG C^C[AT][AT]GG
1611 CCTAGG
TaqI 131 TCGA T^CGA
977 TCGA
TthHB8I 131 TCGA T^CGA
977 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BcnI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI SacI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI