Restriction map for
pFN21AE4489
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstXI Bsu36I CelII CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KspI MamI MluI MluNI
MroI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SfiI
SfoI SfuI SmaI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 948 GGTACC G^GTACC
AccII 1009 CGCG CG^CG
Asp718I 948 GGTACC G^GTACC
AspI 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 948 GGTACC G^G[CT][GA]CC
BanII 80 GAGCTC G[GA]GC[CT]^C
BbsI 442 GAAGAC GAAGAC(2/6)
BbvCI 1497 CCTCAGC CCTCAGC(-5/-2)
BcnI 722 CCCGG CC^[GC]GG
BpmI 152 CTGGAG CTGGAG(16/14)
BpuAI 442 GAAGAC GAAGAC(2/6)
BseRI 821 CTCCTC GAGGAG(10/8)
BsgI 1147 CTGCAC GTGCAG(16/14)
BsmFI 301 GGGAC GGGAC(10/14)
BspDI 529 ATCGAT AT^CGAT
BsrDI 1386 CATTGC GCAATG(2/0)
Bst1107I 981 GTATAC GTA^TAC
BstUI 1009 CGCG CG^CG
BstZ17I 981 GTATAC GTA^TAC
ClaI 529 ATCGAT AT^CGAT
EcoT14I 275 CCAAGG C^C[AT][AT]GG
HphI 1086 TCACC GGTGA(8/7)
KpnI 948 GGTACC GGTAC^C
MaeII 328 ACGT A^CGT
MaeIII 974 GTTAC ^GT[ACGT]AC
MfeI 683 CAATTG C^AATTG
MslI 1243 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 683 CAATTG C^AATTG
MvnI 1009 CGCG CG^CG
NaeI 69 GCCGGC GCC^GGC
NciI 722 CCCGG CC^[GC]GG
NgoMIV 69 GCCGGC G^CCGGC
PaeR7I 1514 CTCGAG C^TCGAG
PflFI 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 418 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1363 CTGCAG CTGCA^G
SacI 80 GAGCTC GAGCT^C
SalI 956 GTCGAC G^TCGAC
SexAI 951 ACCTGGT A^CC[AT]GGT
SmlI 1514 CTCGAG C^T[CT][GA]AG
SpeI 168 ACTAGT A^CTAGT
StyI 275 CCAAGG C^C[AT][AT]GG
Tth111I 763 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1514 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 873 ACATGT A^C[GA][CT]GT
977 ACATGT
AgeI 61 ACCGGT A^CCGGT
363 ACCGGT
AlwI 400 GGATC GGATC(4/5)
1239 GGATC
AlwNI 91 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
146 CAGTATCTG
Asp700I 502 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
913 GAAGCACTTC
AspHI 80 GAGCTC G[AT]GC[AT]^C
1491 GAGCAC
BfaI 169 CTAG C^TAG
551 CTAG
BglII 614 AGATCT A^GATCT
1127 AGATCT
BsiHKAI 80 GAGCTC G[AT]GC[AT]^C
1491 GAGCAC
BsmAI 1423 GAGAC GTCTC(1/5)
1517 GAGAC
BspLU11I 873 ACATGT A^CATGT
977 ACATGT
BstYI 614 AGATCT [GA]^GATC[CT]
1127 AGATCT
DraII 423 AGGTCCT [GA]G^G[ACGT]CC[CT]
1395 GGGTCCT
EaeI 437 TGGCCG [CT]^GGCC[GA]
524 CGGCCA
EarI 943 GAAGAG CTCTTC(1/4)
1476 GAAGAG
EcoO109I 423 AGGTCCT [GA]G^G[ACGT]CC[CT]
1395 GGGTCCT
EcoRV 454 GATATC GAT^ATC
1078 GATATC
FauI 9 GCGGG CCCGC(4/6)
18 GCGGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
93 GGCGCT
HincII 28 GTCAAC GT[CT]^[GA]AC
956 GTCGAC
HindII 28 GTCAAC GT[CT]^[GA]AC
956 GTCGAC
Ksp632I 943 GAAGAG CTCTTC(1/4)
1476 GAAGAG
MaeI 169 CTAG C^TAG
551 CTAG
MflI 614 AGATCT [GA]^GATC[CT]
1127 AGATCT
MseI 188 TTAA T^TAA
1201 TTAA
MspA1I 842 CAGCTG C[AC]G^C[GT]G
1144 CAGCTG
NspI 873 ACATGT [GA]CATG^[CT]
977 ACATGT
PinAI 61 ACCGGT A^CCGGT
363 ACCGGT
PleI 1519 GACTC GAGTC(4/5)
155 GAGTC
PpuMI 423 AGGTCCT [GA]G^G[AT]CC[CT]
1395 GGGTCCT
PvuII 842 CAGCTG CAG^CTG
1144 CAGCTG
SfaNI 15 GATGC GCATC(5/9)
316 GATGC
SfcI 663 CTACAG C^T[GA][CT]AG
1363 CTGCAG
SgrAI 60 CACCGGTG C[GA]^CCGG[CT]G
362 CACCGGTG
Tru9I 188 TTAA T^TAA
1201 TTAA
TspRI 666 CAGTG CA[GC]TG[ACGT][ACGT]^
1413 CACTG
XhoII 614 AGATCT [GA]^GATC[CT]
1127 AGATCT
XmnI 502 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
913 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AvaI AvaII BbvI BmyI
BsaJI BsaWI BsiYI BslI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TfiI TseI Tsp509I TthHB8I