Restriction map for
pFN21AB5784
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BclI
BfrI BlnI BlpI Bpu1102I BseAI
BsgI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcyI 1407 GGCGTC G[GA]^CG[CT]C
AspHI 1469 GTGCTC G[AT]GC[AT]^C
AvaII 1534 GGACC G^G[AT]CC
BamHI 2286 GGATCC G^GATCC
BanI 1737 GGCACC G^G[CT][GA]CC
BbvCI 1858 CCTCAGC CCTCAGC(-5/-2)
BglI 2242 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1046 AGATCT A^GATCT
BsaAI 406 TACGTG [CT]AC^GT[GA]
BsaHI 1407 GGCGTC G[GA]^CG[CT]C
BsaI 251 GGTCTC GGTCTC(1/5)
BsaWI 1055 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1469 GTGCTC G[AT]GC[AT]^C
BsmBI 1386 CGTCTC CGTCTC(1/5)
BspLU11I 653 ACATGT A^CATGT
BspMI 1989 ACCTGC ACCTGC(4/8)
BsrDI 369 CATTGC GCAATG(2/0)
BstAPI 1738 GCACCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1442 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2002 GCGC GCG^C
DraI 2215 TTTAAA TTT^AAA
DrdI 1332 GACTGCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 697 GATATC GAT^ATC
EcoT22I 2195 ATGCAT ATGCA^T
FauI 1999 CCCGC CCCGC(4/6)
HgaI 1408 GCGTC GACGC(5/10)
HhaI 2002 GCGC GCG^C
Hin1I 1407 GGCGTC G[GA]^CG[CT]C
HinP1I 2002 GCGC G^CGC
MaeII 407 ACGT A^CGT
NsiI 2195 ATGCAT ATGCA^T
NspI 653 ACATGT [GA]CATG^[CT]
PflMI 1742 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1435 CTGCAG CTGCA^G
SspI 1789 AATATT AAT^ATT
Van91I 1742 CCAATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 594 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 657 GTCTAC GT^[AC][GT]AC
981 GTCTAC
AccII 2001 CGCG CG^CG
2003 CGCG
AcsI 741 GAATTC [GA]^AATT[CT]
966 GAATTC
AfaI 1307 GTAC GT^AC
1640 GTAC
AflIII 407 ACGTGT A^C[GA][CT]GT
653 ACATGT
AlwNI 1703 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1799 CAGCTCCTG
ApoI 741 GAATTC [GA]^AATT[CT]
966 GAATTC
Asp700I 1158 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2205 GAACCCTTTC
BbsI 1341 GTCTTC GAAGAC(2/6)
1558 GTCTTC
BfaI 595 CTAG C^TAG
1501 CTAG
BpuAI 1341 GTCTTC GAAGAC(2/6)
1558 GTCTTC
BsaBI 106 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2107 GATGCTAATC
BsmAI 252 GTCTC GTCTC(1/5)
1387 GTCTC
BsmI 2085 GAATGC GAATGC(1/-1)
2072 GCATTC
BstUI 2001 CGCG CG^CG
2003 CGCG
DsaI 1087 CCATGG C^C[GA][CT]GG
1537 CCATGG
EcoNI 1204 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1525 CCTCAGGCAGG
EcoRI 741 GAATTC G^AATTC
966 GAATTC
MaeI 595 CTAG C^TAG
1501 CTAG
MamI 106 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2107 GATGCTAATC
MvnI 2001 CGCG CG^CG
2003 CGCG
NcoI 1087 CCATGG C^CATGG
1537 CCATGG
PleI 211 GACTC GAGTC(4/5)
1276 GACTC
PvuII 242 CAGCTG CAG^CTG
1649 CAGCTG
RsaI 1307 GTAC GT^AC
1640 GTAC
SfcI 1427 CTACAG C^T[GA][CT]AG
1435 CTGCAG
XmnI 1158 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2205 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AluI AlwI AocI ApaI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII