Restriction map for
pFN21AE6403
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1442 GGTACC G^GTACC
AccII 588 CGCG CG^CG
AcsI 645 GAATTC [GA]^AATT[CT]
AocI 37 CCTGAGG CC^T[ACGT]AGG
ApaI 257 GGGCCC GGGCC^C
ApoI 645 GAATTC [GA]^AATT[CT]
Asp718I 1442 GGTACC G^GTACC
AspHI 184 GTGCTC G[AT]GC[AT]^C
AspI 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1518 GGATCC G^GATCC
BglII 107 AGATCT A^GATCT
BlpI 1245 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1245 GCTCAGC GC^T[ACGT]AGC
BsaI 208 GGTCTC GGTCTC(1/5)
BseRI 1496 GAGGAG GAGGAG(10/8)
BsgI 1278 CTGCAC GTGCAG(16/14)
BsiEI 1402 CGACCG CG[GA][CT]^CG
BsiHKAI 184 GTGCTC G[AT]GC[AT]^C
BsrBI 1060 GAGCGG CCGCTC(-3/-3)
BstUI 588 CGCG CG^CG
Bsu36I 37 CCTGAGG CC^T[ACGT]AGG
CelII 1245 GCTCAGC GC^T[ACGT]AGC
CfoI 1209 GCGC GCG^C
Eco81I 37 CCTGAGG CC^T[ACGT]AGG
EcoRI 645 GAATTC G^AATTC
EcoT14I 176 CCAAGG C^C[AT][AT]GG
HapII 82 CCGG C^CGG
HhaI 1209 GCGC GCG^C
HinP1I 1209 GCGC G^CGC
HincII 4 GTTGAC GT[CT]^[GA]AC
HindII 4 GTTGAC GT[CT]^[GA]AC
HpaII 82 CCGG C^CGG
KpnI 1442 GGTACC GGTAC^C
MspI 82 CCGG C^CGG
MvnI 588 CGCG CG^CG
PflFI 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 356 GACTC GAGTC(4/5)
PpuMI 1451 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1472 CTGCAG CTGCA^G
SapI 1086 GCTCTTC GCTCTTC(1/4)
SspI 918 AATATT AAT^ATT
StyI 176 CCAAGG C^C[AT][AT]GG
TaqI 1227 TCGA T^CGA
Tth111I 1229 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1227 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaI 156 CTCGGG C^[CT]CG[GA]G
444 CCCGAG
AvaII 362 GGACC G^G[AT]CC
1452 GGTCC
BalI 618 TGGCCA TGG^CCA
872 TGGCCA
BanI 280 GGCACC G^G[CT][GA]CC
1442 GGTACC
BbsI 1126 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BfaI 853 CTAG C^TAG
995 CTAG
BpmI 1447 CTCCAG CTGGAG(16/14)
322 CTGGAG
BpuAI 1126 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BsmFI 725 GGGAC GGGAC(10/14)
1235 GTCCC
BsoBI 156 CTCGGG C^[CT]CG[GA]G
444 CCCGAG
BspMI 773 ACCTGC ACCTGC(4/8)
87 GCAGGT
BstXI 419 CCAACTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1283 CCATCTTCCTGG
BstYI 107 AGATCT [GA]^GATC[CT]
1518 GGATCC
EaeI 618 TGGCCA [CT]^GGCC[GA]
872 TGGCCA
MaeI 853 CTAG C^TAG
995 CTAG
MflI 107 AGATCT [GA]^GATC[CT]
1518 GGATCC
MluNI 618 TGGCCA TGG^CCA
872 TGGCCA
MscI 618 TGGCCA TGG^CCA
872 TGGCCA
SfcI 1152 CTACAG C^T[GA][CT]AG
1472 CTGCAG
SmlI 1014 CTCAAG C^T[CT][GA]AG
1240 CTCAAG
StuI 326 AGGCCT AGG^CCT
1356 AGGCCT
TfiI 338 GATTC G^A[AT]TC
759 GAATC
Tsp509I 646 AATT ^AATT
933 AATT
XcmI 470 CCAGCCGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1283 CCATCTTCCTGGTGG
XhoII 107 AGATCT [GA]^GATC[CT]
1518 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
TspRI