Restriction map for
pFN21AE5228
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BseRI BsiEI BsiWI BsmI
Bsp1407I BspLU11I BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I CelII CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MaeIII
MfeI MflI MluI MluNI MscI
MseI MspA1I MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 159 CGCG CG^CG
AccIII 689 TCCGGA T^CCGGA
AcyI 415 GACGCC G[GA]^CG[CT]C
AlwNI 262 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 505 GTGCTC G[AT]GC[AT]^C
AvaI 528 CTCGGG C^[CT]CG[GA]G
AvaII 348 GGACC G^G[AT]CC
BclI 641 TGATCA T^GATCA
BcnI 596 CCGGG CC^[GC]GG
BmyI 505 GTGCTC G[AGT]GC[ACT]^C
BsaBI 642 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 415 GACGCC G[GA]^CG[CT]C
BsaWI 689 TCCGGA [AT]^CCGG[AT]
BseAI 689 TCCGGA T^CCGGA
BsgI 15 CTGCAC GTGCAG(16/14)
BsiHKAI 505 GTGCTC G[AT]GC[AT]^C
BsmBI 454 GAGACG CGTCTC(1/5)
BsoBI 528 CTCGGG C^[CT]CG[GA]G
Bsp1286I 505 GTGCTC G[AGT]GC[ACT]^C
BspDI 580 ATCGAT AT^CGAT
BspEI 689 TCCGGA T^CCGGA
BsrDI 618 CATTGC GCAATG(2/0)
BsrFI 322 GCCGGC [GA]^CCGG[CT]
BsrI 261 CCAGT ACTGG(1/-1)
BssSI 451 CACGAG CACGAG(-5/-1)
BstAPI 17 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 159 CGCG CG^CG
BstXI 428 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 322 GCCGGC [GA]^CCGG[CT]
ClaI 580 ATCGAT AT^CGAT
DraII 386 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 18 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 401 CCGTGG C^C[GA][CT]GG
EaeI 317 TGGCCG [CT]^GGCC[GA]
EcoO109I 386 AGGCCCT [GA]G^G[ACGT]CC[CT]
FbaI 641 TGATCA T^GATCA
HaeII 205 GGCGCT [GA]GCGC^[CT]
Hin1I 415 GACGCC G[GA]^CG[CT]C
HincII 340 GTCAAC GT[CT]^[GA]AC
HindII 340 GTCAAC GT[CT]^[GA]AC
HinfI 256 GAATC G^A[ACGT]TC
MaeII 277 ACGT A^CGT
MamI 642 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 689 TCCGGA T^CCGGA
MvnI 159 CGCG CG^CG
NaeI 322 GCCGGC GCC^GGC
NciI 596 CCGGG CC^[GC]GG
NgoMIV 322 GCCGGC G^CCGGC
SapI 39 GCTCTTC GCTCTTC(1/4)
SfcI 391 CTACAG C^T[GA][CT]AG
SmlI 508 CTCAAG C^T[CT][GA]AG
TfiI 256 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 81 GATCC GGATC(4/5)
660 GATCC
Asp700I 369 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
441 GAAGACCTTC
BsmAI 149 GAGAC GTCTC(1/5)
454 GAGAC
BsmFI 274 GGGAC GGGAC(10/14)
524 GTCCC
BspHI 212 TCATGA T^CATGA
644 TCATGA
BspMI 35 ACCTGC ACCTGC(4/8)
286 ACCTGC
CfoI 160 GCGC GCG^C
206 GCGC
DdeI 501 CTCAG C^T[ACGT]AG
664 CTGAG
EcoNI 390 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
552 CCTCTTCAAGG
EcoT14I 359 CCAAGG C^C[AT][AT]GG
428 CCAAGG
FauI 114 CCCGC CCCGC(4/6)
157 CCCGC
HgaI 415 GACGC GACGC(5/10)
456 GACGC
HhaI 160 GCGC GCG^C
206 GCGC
HinP1I 160 GCGC G^CGC
206 GCGC
HphI 438 GGTGA GGTGA(8/7)
284 TCACC
MslI 195 CATCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
498 CACCTCAGTG
RcaI 212 TCATGA T^CATGA
644 TCATGA
StyI 359 CCAAGG C^C[AT][AT]GG
428 CCAAGG
TaqI 581 TCGA T^CGA
653 TCGA
Tsp509I 52 AATT ^AATT
517 AATT
TthHB8I 581 TCGA T^CGA
653 TCGA
XmnI 369 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
441 GAAGACCTTC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BpmI BpuAI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI Ksp632I
MboI MboII MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI