Restriction map for
pFN21AE9258
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstXI
BstYI Bsu36I CelII ClaI CpoI
DdeI DraII DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tsp45I
TthHB8I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcsI 557 AAATTC [GA]^AATT[CT]
AcyI 372 GGCGCC G[GA]^CG[CT]C
AflIII 392 ACACGT A^C[GA][CT]GT
AgeI 152 ACCGGT A^CCGGT
ApoI 557 AAATTC [GA]^AATT[CT]
AspHI 140 GAGCAC G[AT]GC[AT]^C
AspI 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 150 GGACC G^G[AT]CC
BanI 372 GGCGCC G^G[CT][GA]CC
BbeI 372 GGCGCC GGCGC^C
BclI 713 TGATCA T^GATCA
BglI 53 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 140 GAGCAC G[AGT]GC[ACT]^C
BsaHI 372 GGCGCC G[GA]^CG[CT]C
BsaWI 152 ACCGGT [AT]^CCGG[AT]
BseRI 93 GAGGAG GAGGAG(10/8)
BsiHKAI 140 GAGCAC G[AT]GC[AT]^C
Bsp1286I 140 GAGCAC G[AGT]GC[ACT]^C
BsrBI 551 GAGCGG CCGCTC(-3/-3)
BsrDI 806 GCAATG GCAATG(2/0)
Bst1107I 621 GTATAC GTA^TAC
BstZ17I 621 GTATAC GTA^TAC
DpnII 714 GATC ^GATC
DraI 507 TTTAAA TTT^AAA
DrdI 412 GACTTCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 57 CCGCGG C^C[GA][CT]GG
EaeI 38 TGGCCG [CT]^GGCC[GA]
EcoNI 206 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 35 CCTTGG C^C[AT][AT]GG
EcoT22I 775 ATGCAT ATGCA^T
FauI 68 GCGGG CCCGC(4/6)
FbaI 713 TGATCA T^GATCA
HaeII 372 GGCGCC [GA]GCGC^[CT]
Hin1I 372 GGCGCC G[GA]^CG[CT]C
HphI 711 GGTGA GGTGA(8/7)
KasI 372 GGCGCC G^GCGCC
KspI 57 CCGCGG CCGC^GG
MaeII 394 ACGT A^CGT
MboI 714 GATC ^GATC
MspA1I 57 CCGCGG C[AC]G^C[GT]G
NaeI 369 GCCGGC GCC^GGC
NarI 372 GGCGCC GG^CGCC
NdeII 714 GATC ^GATC
NgoMIV 369 GCCGGC G^CCGGC
NsiI 775 ATGCAT ATGCA^T
NspI 777 GCATGT [GA]CATG^[CT]
PflFI 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 152 ACCGGT A^CCGGT
PshAI 108 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 57 CCGCGG CCGC^GG
Sau3AI 714 GATC ^GATC
SfaNI 774 GATGC GCATC(5/9)
SfoI 372 GGCGCC GGC^GCC
StuI 197 AGGCCT AGG^CCT
StyI 35 CCTTGG C^C[AT][AT]GG
Tsp509I 558 AATT ^AATT
Tth111I 412 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 424 GTCTAC GT^[AC][GT]AC
621 GTATAC
BbsI 690 GAAGAC GAAGAC(2/6)
853 GAAGAC
BcnI 110 CCGGG CC^[GC]GG
228 CCCGG
BpuAI 690 GAAGAC GAAGAC(2/6)
853 GAAGAC
BsgI 135 GTGCAG GTGCAG(16/14)
698 GTGCAG
BsmAI 389 GAGAC GTCTC(1/5)
410 GAGAC
BsrFI 152 ACCGGT [GA]^CCGG[CT]
369 GCCGGC
CfoI 329 GCGC GCG^C
373 GCGC
Cfr10I 152 ACCGGT [GA]^CCGG[CT]
369 GCCGGC
HhaI 329 GCGC GCG^C
373 GCGC
HinP1I 329 GCGC G^CGC
373 GCGC
MseI 173 TTAA T^TAA
508 TTAA
NciI 110 CCGGG CC^[GC]GG
228 CCCGG
PflMI 304 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGGGGATGG
TfiI 268 GAATC G^A[AT]TC
476 GAATC
Tru9I 173 TTAA T^TAA
508 TTAA
Van91I 304 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BbvI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstUI Cac8I Cfr13I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MboII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV PleI Sau96I ScrFI TseI
TspRI