Restriction map for
pFN21AE4544
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BcnI BglI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HpaI
HpaII KasI Ksp632I KspI MluI
MroI MspA1I MspI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 802 GGTACC G^GTACC
AflII 49 CTTAAG C^TTAAG
AflIII 408 ACGTGT A^C[GA][CT]GT
AlwNI 926 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 199 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 802 GGTACC G^GTACC
BalI 1434 TGGCCA TGG^CCA
BanII 332 GAGCCC G[GA]GC[CT]^C
BbvI 1161 GCTGC GCAGC(8/12)
BclI 1754 TGATCA T^GATCA
BfrI 49 CTTAAG C^TTAAG
BmyI 332 GAGCCC G[AGT]GC[ACT]^C
BpmI 1775 CTCCAG CTGGAG(16/14)
BsaAI 407 TACGTG [CT]AC^GT[GA]
BseRI 1067 CTCCTC GAGGAG(10/8)
BsiEI 813 CGGTCG CG[GA][CT]^CG
BsmAI 274 GAGAC GTCTC(1/5)
Bsp1286I 332 GAGCCC G[AGT]GC[ACT]^C
BsrDI 1141 CATTGC GCAATG(2/0)
Cac8I 176 GCCTGC GC[ACGT]^[ACGT]GC
DrdI 655 GACATTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1801 CCGTGG C^C[GA][CT]GG
EaeI 1434 TGGCCA [CT]^GGCC[GA]
EcoRI 753 GAATTC G^AATTC
EcoT22I 1793 ATGCAT ATGCA^T
FauI 1335 CCCGC CCCGC(4/6)
FbaI 1754 TGATCA T^GATCA
Fnu4HI 1161 GCTGC GC^[ACGT]GC
HaeIII 1435 GGCC GG^CC
HincII 986 GTCAAC GT[CT]^[GA]AC
HindII 986 GTCAAC GT[CT]^[GA]AC
HindIII 708 AAGCTT A^AGCTT
ItaI 1161 GCTGC GC^[ACGT]GC
KpnI 802 GGTACC GGTAC^C
MfeI 62 CAATTG C^AATTG
MluNI 1434 TGGCCA TGG^CCA
MscI 1434 TGGCCA TGG^CCA
MunI 62 CAATTG C^AATTG
NdeI 427 CATATG CA^TATG
NsiI 1793 ATGCAT ATGCA^T
ScaI 1659 AGTACT AGT^ACT
SfcI 723 CTATAG C^T[GA][CT]AG
SmlI 49 CTTAAG C^T[CT][GA]AG
TaqI 1399 TCGA T^CGA
TseI 1161 GCTGC G^C[AT]GC
TthHB8I 1399 TCGA T^CGA
XcmI 568 CCATTTCCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 199 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AccI 664 GTCTAC GT^[AC][GT]AC
1352 GTCTAC
AciI 1080 CCGC CCGC(-3/-1)
1336 CCGC
BanI 556 GGCACC G^G[CT][GA]CC
802 GGTACC
BbsI 1115 GAAGAC GAAGAC(2/6)
1441 GAAGAC
BfaI 1520 CTAG C^TAG
1663 CTAG
BglII 691 AGATCT A^GATCT
1429 AGATCT
BpuAI 1115 GAAGAC GAAGAC(2/6)
1441 GAAGAC
BsaJI 1550 CCCTGG C^C[ACGT][ACGT]GG
1801 CCGTGG
BsmI 1810 GAATGC GAATGC(1/-1)
903 GCATTC
BsrI 1596 ACTGG ACTGG(1/-1)
806 CCAGT
DraIII 1040 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1376 CACTCAGTG
MaeI 1520 CTAG C^TAG
1663 CTAG
MaeII 313 ACGT A^CGT
408 ACGT
MwoI 350 GCACTTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
941 GCTTTCCAAGC
PflMI 387 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
946 CCAAGCTCTGG
PleI 276 GACTC GAGTC(4/5)
584 GAGTC
Van91I 387 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
946 CCAAGCTCTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BsaBI BsiYI BslI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII FokI HinfI HphI
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I Tsp45I
Tsp509I TspRI XhoII