Restriction map for
pFN21AE5701
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BsaAI BsaBI BsaHI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoNI EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 1411 CGCG CG^CG
AlwNI 1621 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 862 CCTGAGG CC^T[ACGT]AGG
ApaI 314 GGGCCC GGGCC^C
AvrII 1065 CCTAGG C^CTAGG
BalI 2039 TGGCCA TGG^CCA
BamHI 432 GGATCC G^GATCC
BglII 1667 AGATCT A^GATCT
BlnI 1065 CCTAGG C^CTAGG
BsaI 424 GAGACC GGTCTC(1/5)
BsaWI 1106 TCCGGT [AT]^CCGG[AT]
BsiEI 1709 CGGTCG CG[GA][CT]^CG
BsmBI 1962 GAGACG CGTCTC(1/5)
BsrDI 1203 CATTGC GCAATG(2/0)
BsrFI 32 GCCGGC [GA]^CCGG[CT]
BssSI 1308 CTCGTG CACGAG(-5/-1)
BstAPI 468 GCACGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 904 GGTGACC G^GT[ACGT]ACC
BstPI 904 GGTGACC G^GT[ACGT]ACC
BstUI 1411 CGCG CG^CG
Bsu36I 862 CCTGAGG CC^T[ACGT]AGG
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
DraIII 469 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1162 GACTGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 862 CCTGAGG CC^T[ACGT]AGG
EcoO65I 904 GGTGACC G^GT[ACGT]ACC
EcoRI 1777 GAATTC G^AATTC
HgaI 181 GACGC GACGC(5/10)
HindIII 2235 AAGCTT A^AGCTT
MaeII 151 ACGT A^CGT
MluNI 2039 TGGCCA TGG^CCA
MscI 2039 TGGCCA TGG^CCA
MspA1I 540 CAGCTG C[AC]G^C[GT]G
MvnI 1411 CGCG CG^CG
NaeI 32 GCCGGC GCC^GGC
NdeI 1657 CATATG CA^TATG
NgoMIV 32 GCCGGC G^CCGGC
PaeR7I 1738 CTCGAG C^TCGAG
PflMI 647 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 540 CAGCTG CAG^CTG
SacI 2143 GAGCTC GAGCT^C
SapI 2178 GCTCTTC GCTCTTC(1/4)
SmaI 716 CCCGGG CCC^GGG
Van91I 647 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1738 CTCGAG C^TCGAG
XmaCI 716 CCCGGG C^CCGGG
XmaI 716 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 802 GTCTAC GT^[AC][GT]AC
1924 GTAGAC
AcsI 256 AAATTT [GA]^AATT[CT]
1777 GAATTC
AfaI 1176 GTAC GT^AC
1647 GTAC
AlwI 433 GATCC GGATC(4/5)
432 GGATC
Aor51HI 941 AGCGCT AGC^GCT
1353 AGCGCT
ApoI 256 AAATTT [GA]^AATT[CT]
1777 GAATTC
Asp700I 457 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2175 GAAGCTCTTC
AspHI 1041 GTGCTC G[AT]GC[AT]^C
2143 GAGCTC
BanI 858 GGTGCC G^G[CT][GA]CC
1057 GGCACC
BanII 314 GGGCCC G[GA]GC[CT]^C
2143 GAGCTC
BbsI 1491 GAAGAC GAAGAC(2/6)
1888 GAAGAC
BfaI 830 CTAG C^TAG
1066 CTAG
BlpI 1836 GCTCAGC GC^T[ACGT]AGC
2145 GCTCAGC
Bpu1102I 1836 GCTCAGC GC^T[ACGT]AGC
2145 GCTCAGC
BpuAI 1491 GAAGAC GAAGAC(2/6)
1888 GAAGAC
BseRI 1975 GAGGAG GAGGAG(10/8)
1996 GAGGAG
BsiHKAI 1041 GTGCTC G[AT]GC[AT]^C
2143 GAGCTC
BstF5I 1860 CATCC GGATG(2/0)
381 GGATG
BstYI 432 GGATCC [GA]^GATC[CT]
1667 AGATCT
CelII 1836 GCTCAGC GC^T[ACGT]AGC
2145 GCTCAGC
Eco47III 941 AGCGCT AGC^GCT
1353 AGCGCT
EcoRV 223 GATATC GAT^ATC
1402 GATATC
FokI 1860 CATCC GGATG(9/13)
381 GGATG
HaeII 941 AGCGCT [GA]GCGC^[CT]
1353 AGCGCT
MaeI 830 CTAG C^TAG
1066 CTAG
MflI 432 GGATCC [GA]^GATC[CT]
1667 AGATCT
NcoI 1049 CCATGG C^CATGG
1343 CCATGG
PpuMI 67 GGGACCC [GA]G^G[AT]CC[CT]
205 AGGTCCT
RsaI 1176 GTAC GT^AC
1647 GTAC
XcmI 1334 CCAGCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2120 CCACACATGACATGG
XhoII 432 GGATCC [GA]^GATC[CT]
1667 AGATCT
XmnI 457 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2175 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I