Restriction map for
pFN21AE4423
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# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BssHII BstBI BstEII
BstPI BstUI Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT14I EcoT22I FauI
FbaI Fnu4HI FseI FspI HapII
Hin1I HincII HindII HpaI HpaII
ItaI KasI KpnI KspI MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI TseI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 1284 CCGC CCGC(-3/-1)
Asp700I 1196 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 689 TGGCCA TGG^CCA
BamHI 875 GGATCC G^GATCC
BmyI 77 GGGCAC G[AGT]GC[ACT]^C
BpmI 600 CTCCAG CTGGAG(16/14)
BseRI 356 GAGGAG GAGGAG(10/8)
BsmI 992 GAATGC GAATGC(1/-1)
Bsp1286I 77 GGGCAC G[AGT]GC[ACT]^C
BssSI 8 CTCGTG CACGAG(-5/-1)
Bst1107I 985 GTATAC GTA^TAC
BstAPI 841 GCAAAGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 985 GTATAC GTA^TAC
CfoI 51 GCGC GCG^C
DraI 1027 TTTAAA TTT^AAA
DraII 386 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1042 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 871 CCGTGG C^C[GA][CT]GG
EaeI 689 TGGCCA [CT]^GGCC[GA]
EarI 271 GAAGAG CTCTTC(1/4)
EcoO109I 386 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeII 50 AGCGCC [GA]GCGC^[CT]
HgaI 544 GACGC GACGC(5/10)
HhaI 51 GCGC GCG^C
HinP1I 51 GCGC G^CGC
HindIII 1032 AAGCTT A^AGCTT
Ksp632I 271 GAAGAG CTCTTC(1/4)
MluNI 689 TGGCCA TGG^CCA
MscI 689 TGGCCA TGG^CCA
MspA1I 457 CAGCTG C[AC]G^C[GT]G
NheI 3 GCTAGC G^CTAGC
PpuMI 386 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 457 CAGCTG CAG^CTG
SfcI 480 CTACAG C^T[GA][CT]AG
SmlI 622 CTCAAG C^T[CT][GA]AG
TaqI 1214 TCGA T^CGA
TthHB8I 1214 TCGA T^CGA
XmnI 1196 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 541 GTAGAC GT^[AC][GT]AC
985 GTATAC
AvaII 195 GGACC G^G[AT]CC
387 GGTCC
BbsI 666 GAAGAC GAAGAC(2/6)
964 GTCTTC
BpuAI 666 GAAGAC GAAGAC(2/6)
964 GTCTTC
BsgI 571 CTGCAC GTGCAG(16/14)
734 CTGCAC
BsmFI 430 GGGAC GGGAC(10/14)
388 GTCCC
Bsp1407I 999 TGTACA T^GTACA
1267 TGTACA
BsrGI 999 TGTACA T^GTACA
1267 TGTACA
BstF5I 554 CATCC GGATG(2/0)
1232 CATCC
BstXI 241 CCAGCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
391 CCAGAGTGGTGG
BstYI 301 GGATCT [GA]^GATC[CT]
875 GGATCC
Cac8I 3 GCTAGC GC[ACGT]^[ACGT]GC
402 GCTTGC
EcoRV 719 GATATC GAT^ATC
1111 GATATC
FokI 554 CATCC GGATG(9/13)
1232 CATCC
HphI 14 TCACC GGTGA(8/7)
515 TCACC
MaeII 30 ACGT A^CGT
1076 ACGT
MflI 301 GGATCT [GA]^GATC[CT]
875 GGATCC
NspI 639 ACATGC [GA]CATG^[CT]
886 GCATGT
SspBI 999 TGTACA T^GTACA
1267 TGTACA
XcmI 241 CCAGCCTTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
744 CCACAGCAGTTTTGG
XhoII 301 GGATCT [GA]^GATC[CT]
875 GGATCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BsaJI BsiYI BslI BsrI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII HaeIII HinfI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp45I Tsp509I TspRI