Restriction map for
pFN21AE6208
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI AluI AlwI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI NaeI
NarI NciI NgoMIV NheI NlaIV
NotI NruI NsiI NspI PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
TspRI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 476 CGCG CG^CG
AcsI 77 AAATTC [GA]^AATT[CT]
Alw44I 346 GTGCAC G^TGCAC
AlwNI 467 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 346 GTGCAC G^TGCAC
ApoI 77 AAATTC [GA]^AATT[CT]
Asp700I 73 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 346 GTGCAC G[AT]GC[AT]^C
AvaII 261 GGACC G^G[AT]CC
BbvI 466 GCAGC GCAGC(8/12)
BcgI 87 GCAGATTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 346 GTGCAC G[AGT]GC[ACT]^C
BsiHKAI 346 GTGCAC G[AT]GC[AT]^C
BsmAI 100 GAGAC GTCTC(1/5)
BsmFI 567 GTCCC GGGAC(10/14)
Bsp1286I 346 GTGCAC G[AGT]GC[ACT]^C
BspMI 506 ACCTGC ACCTGC(4/8)
BsrFI 194 ACCGGC [GA]^CCGG[CT]
BsrI 570 CCAGT ACTGG(1/-1)
BstAPI 466 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 301 TTCGAA TT^CGAA
BstUI 476 CGCG CG^CG
Cac8I 477 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 475 GCGC GCG^C
Cfr10I 194 ACCGGC [GA]^CCGG[CT]
Cfr13I 261 GGACC G^G[ACGT]CC
EcoT14I 30 CCATGG C^C[AT][AT]GG
Fnu4HI 466 GCAGC GC^[ACGT]GC
HaeIII 457 GGCC GG^CC
HapII 195 CCGG C^CGG
HhaI 475 GCGC GCG^C
HinP1I 475 GCGC G^CGC
HpaII 195 CCGG C^CGG
HphI 369 GGTGA GGTGA(8/7)
ItaI 466 GCAGC GC^[ACGT]GC
MaeIII 140 GTAAC ^GT[ACGT]AC
MspI 195 CCGG C^CGG
MvnI 476 CGCG CG^CG
NcoI 30 CCATGG C^CATGG
NdeI 334 CATATG CA^TATG
NspV 301 TTCGAA TT^CGAA
PflMI 360 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I 261 GGACC G^G[ACGT]CC
SfuI 301 TTCGAA TT^CGAA
SmlI 118 CTCAAG C^T[CT][GA]AG
StyI 30 CCATGG C^C[AT][AT]GG
TseI 466 GCAGC G^C[AT]GC
Van91I 360 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 73 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BfaI 46 CTAG C^TAG
112 CTAG
BpmI 358 CTCCAG CTGGAG(16/14)
439 CTGGAG
BssKI 270 CCAGG ^CC[ACGT]GG
360 CCAGG
BstNI 270 CCAGG CC^[AT]GG
360 CCAGG
DdeI 237 CTCAG C^T[ACGT]AG
416 CTCAG
DsaI 30 CCATGG C^C[GA][CT]GG
40 CCACGG
EcoNI 264 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
357 CCTCCAGGAGG
EcoRII 270 CCAGG ^CC[AT]GG
360 CCAGG
FauI 123 GCGGG CCCGC(4/6)
477 GCGGG
MaeI 46 CTAG C^TAG
112 CTAG
MboII 73 GAAGA GAAGA(8/7)
329 TCTTC
MvaI 270 CCAGG CC^[AT]GG
360 CCAGG
MwoI 466 GCAGCCCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
481 GCTGTGTCTGC
NlaIII 31 CATG CATG^
399 CATG
ScrFI 270 CCAGG CC^[ACGT]GG
360 CCAGG
TaqI 96 TCGA T^CGA
302 TCGA
Tsp509I 78 AATT ^AATT
317 AATT
TthHB8I 96 TCGA T^CGA
302 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AciI BsaJI BsiYI BslI BstF5I
DpnII FokI HinfI MboI MnlI
NdeII Sau3AI TfiI