Restriction map for
pFN21AE2723
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MspA1I MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 859 AAATTC [GA]^AATT[CT]
AfaI 309 GTAC GT^AC
ApoI 859 AAATTC [GA]^AATT[CT]
Asp700I 885 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 844 CTCGAG C^[CT]CG[GA]G
BalI 501 TGGCCA TGG^CCA
BbeI 184 GGCGCC GGCGC^C
BmyI 258 GGGCAC G[AGT]GC[ACT]^C
BpmI 487 CTGGAG CTGGAG(16/14)
BsoBI 844 CTCGAG C^[CT]CG[GA]G
Bsp1286I 258 GGGCAC G[AGT]GC[ACT]^C
BspMI 877 ACCTGC ACCTGC(4/8)
BsrFI 130 ACCGGC [GA]^CCGG[CT]
BssHII 20 GCGCGC G^CGCGC
Cfr10I 130 ACCGGC [GA]^CCGG[CT]
EarI 387 GAAGAG CTCTTC(1/4)
EcoT22I 799 ATGCAT ATGCA^T
KasI 184 GGCGCC G^GCGCC
Ksp632I 387 GAAGAG CTCTTC(1/4)
MaeII 119 ACGT A^CGT
MaeIII 344 GTGAC ^GT[ACGT]AC
MboII 387 GAAGA GAAGA(8/7)
MluNI 501 TGGCCA TGG^CCA
MscI 501 TGGCCA TGG^CCA
NarI 184 GGCGCC GG^CGCC
NsiI 799 ATGCAT ATGCA^T
NspI 801 GCATGC [GA]CATG^[CT]
PaeR7I 844 CTCGAG C^TCGAG
PpuMI 101 GGGACCT [GA]G^G[AT]CC[CT]
PstI 493 CTGCAG CTGCA^G
RsaI 309 GTAC GT^AC
SapI 387 GAAGAGC GCTCTTC(1/4)
SfoI 184 GGCGCC GGC^GCC
SgrAI 129 CACCGGCG C[GA]^CCGG[CT]G
SphI 801 GCATGC GCATG^C
StuI 467 AGGCCT AGG^CCT
Tsp45I 344 GTGAC ^GT[GC]AC
Tsp509I 860 AATT ^AATT
XhoI 844 CTCGAG C^TCGAG
XmnI 885 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 152 GGCGTC G[GA]^CG[CT]C
184 GGCGCC
AlwI 246 GATCC GGATC(4/5)
852 GATCC
BanI 184 GGCGCC G^G[CT][GA]CC
550 GGTGCC
BsaHI 152 GGCGTC G[GA]^CG[CT]C
184 GGCGCC
BsmAI 232 GAGAC GTCTC(1/5)
730 GAGAC
BsmBI 232 GAGACG CGTCTC(1/5)
730 GAGACG
BsmFI 101 GGGAC GGGAC(10/14)
426 GTCCC
BsrI 78 ACTGG ACTGG(1/-1)
450 CCAGT
BstAPI 260 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
532 GCACTGTCTGC
BstYI 245 AGATCC [GA]^GATC[CT]
851 AGATCC
DraII 101 GGGACCT [GA]G^G[ACGT]CC[CT]
773 AGGCCCT
DraIII 261 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
505 CACATCGTG
EaeI 317 CGGCCA [CT]^GGCC[GA]
501 TGGCCA
EcoO109I 101 GGGACCT [GA]G^G[ACGT]CC[CT]
773 AGGCCCT
HaeII 184 GGCGCC [GA]GCGC^[CT]
637 AGCGCC
HgaI 153 GCGTC GACGC(5/10)
577 GCGTC
Hin1I 152 GGCGTC G[GA]^CG[CT]C
184 GGCGCC
HinfI 632 GAGTC G^A[ACGT]TC
692 GAGTC
MflI 245 AGATCC [GA]^GATC[CT]
851 AGATCC
NcoI 381 CCATGG C^CATGG
790 CCATGG
PleI 632 GAGTC GAGTC(4/5)
692 GAGTC
SfaNI 412 GATGC GCATC(5/9)
146 GCATC
SfcI 40 CTACAG C^T[GA][CT]AG
493 CTGCAG
SmlI 421 CTCAAG C^T[CT][GA]AG
844 CTCGAG
TaqI 557 TCGA T^CGA
845 TCGA
TthHB8I 557 TCGA T^CGA
845 TCGA
XhoII 245 AGATCC [GA]^GATC[CT]
851 AGATCC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaJI BseRI BsgI BsiYI
BslI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MnlI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI StyI TseI TspRI