Restriction map for
pFN21AE1439
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI Ksp632I MaeI MaeII
MaeIII MamI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AhdI 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 381 GATCC GGATC(4/5)
AlwNI 117 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 5 CTCGGG C^[CT]CG[GA]G
AvaII 132 GGACC G^G[AT]CC
BanI 94 GGCACC G^G[CT][GA]CC
BcnI 77 CCGGG CC^[GC]GG
BsaI 346 GAGACC GGTCTC(1/5)
BsiEI 172 CGACCG CG[GA][CT]^CG
BsmBI 303 GAGACG CGTCTC(1/5)
BsoBI 5 CTCGGG C^[CT]CG[GA]G
BssSI 37 CTCGTG CACGAG(-5/-1)
BstF5I 240 CATCC GGATG(2/0)
DdeI 256 CTGAG C^T[ACGT]AG
DpnII 381 GATC ^GATC
EaeI 74 TGGCCG [CT]^GGCC[GA]
Eam1105I 151 GACTGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 243 CCTTGG C^C[AT][AT]GG
FauI 110 CCCGC CCCGC(4/6)
FokI 240 CATCC GGATG(9/13)
HgaI 157 GCGTC GACGC(5/10)
HphI 329 TCACC GGTGA(8/7)
KspI 111 CCGCGG CCGC^GG
MboI 381 GATC ^GATC
NciI 77 CCGGG CC^[GC]GG
NdeII 381 GATC ^GATC
NlaIII 147 CATG CATG^
PstI 203 CTGCAG CTGCA^G
PvuII 368 CAGCTG CAG^CTG
SacII 111 CCGCGG CCGC^GG
Sau3AI 381 GATC ^GATC
SfaNI 308 GATGC GCATC(5/9)
SfcI 203 CTGCAG C^T[GA][CT]AG
StyI 243 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AluI 118 AGCT AG^CT
369 AGCT
BanII 9 GGGCTC G[GA]GC[CT]^C
46 GGGCTC
BmyI 9 GGGCTC G[AGT]GC[ACT]^C
46 GGGCTC
BpmI 123 CTGGAG CTGGAG(16/14)
300 CTGGAG
BseRI 34 CTCCTC GAGGAG(10/8)
343 GAGGAG
BsmAI 303 GAGAC GTCTC(1/5)
346 GAGAC
Bsp1286I 9 GGGCTC G[AGT]GC[ACT]^C
46 GGGCTC
BsrFI 20 GCCGGT [GA]^CCGG[CT]
349 ACCGGC
BstAPI 146 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
208 GCACCTCCTGC
Cfr10I 20 GCCGGT [GA]^CCGG[CT]
349 ACCGGC
Cfr13I 132 GGACC G^G[ACGT]CC
236 GGCCC
DsaI 71 CCGTGG C^C[GA][CT]GG
111 CCGCGG
HaeIII 75 GGCC GG^CC
236 GGCC
MspA1I 111 CCGCGG C[AC]G^C[GT]G
368 CAGCTG
NlaIV 32 GGCTCC GG[ACGT]^[ACGT]CC
94 GGCACC
Sau96I 132 GGACC G^G[ACGT]CC
236 GGCCC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI BbvI BsaJI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI EcoRII Fnu4HI HaeII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvaI MvnI MwoI ScrFI
TseI