Restriction map for
pFN21AE5212
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I MamI MfeI
MluNI MroI MscI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 436 GACGTC GACGT^C
AcyI 436 GACGTC G[GA]^CG[CT]C
AflIII 122 ACGCGT A^C[GA][CT]GT
AhdI 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 57 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 295 AGCGCT AGC^GCT
AspEI 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 75 CCCGAG C^[CT]CG[GA]G
AvaII 197 GGTCC G^G[AT]CC
BsaAI 112 TACGTG [CT]AC^GT[GA]
BsaHI 436 GACGTC G[GA]^CG[CT]C
BsgI 25 GTGCAG GTGCAG(16/14)
BsoBI 75 CCCGAG C^[CT]CG[GA]G
BspHI 416 TCATGA T^CATGA
BsrBI 387 CCGCTC CCGCTC(-3/-3)
BstAPI 56 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 96 GGTCACC G^GT[ACGT]ACC
BstF5I 141 CATCC GGATG(2/0)
BstPI 96 GGTCACC G^GT[ACGT]ACC
CfoI 296 GCGC GCG^C
DraII 196 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 103 CCGCGG C^C[GA][CT]GG
EaeI 351 TGGCCG [CT]^GGCC[GA]
Eam1105I 436 GACGTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 295 AGCGCT AGC^GCT
EcoO109I 196 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 96 GGTCACC G^GT[ACGT]ACC
EcoT14I 533 CCAAGG C^C[AT][AT]GG
FauI 102 CCCGC CCCGC(4/6)
FokI 141 CATCC GGATG(9/13)
HaeII 295 AGCGCT [GA]GCGC^[CT]
HgaI 124 GCGTC GACGC(5/10)
HhaI 296 GCGC GCG^C
Hin1I 436 GACGTC G[GA]^CG[CT]C
HinP1I 296 GCGC G^CGC
HincII 211 GTCAAC GT[CT]^[GA]AC
HindII 211 GTCAAC GT[CT]^[GA]AC
HinfI 238 GACTC G^A[ACGT]TC
KspI 103 CCGCGG CCGC^GG
MaeIII 97 GTCAC ^GT[ACGT]AC
MboII 429 GAAGA GAAGA(8/7)
MluI 122 ACGCGT A^CGCGT
MslI 168 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV 481 GGCCCC GG[ACGT]^[ACGT]CC
PflMI 330 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 238 GACTC GAGTC(4/5)
PpuMI 196 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 151 CTGCAG CTGCA^G
RcaI 416 TCATGA T^CATGA
SacI 16 GAGCTC GAGCT^C
SacII 103 CCGCGG CCGC^GG
StyI 533 CCAAGG C^C[AT][AT]GG
TaqI 14 TCGA T^CGA
Tsp45I 97 GTCAC ^GT[GC]AC
TspRI 367 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 14 TCGA T^CGA
Van91I 330 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 30 GGATC GGATC(4/5)
107 GGATC
AspHI 16 GAGCTC G[AT]GC[AT]^C
358 GTGCTC
BanII 6 GGGCTC G[GA]GC[CT]^C
16 GAGCTC
BbvCI 291 CCTCAGC CCTCAGC(-5/-2)
187 GCTGAGG
BcnI 354 CCGGG CC^[GC]GG
372 CCGGG
BsiHKAI 16 GAGCTC G[AT]GC[AT]^C
358 GTGCTC
BspMI 41 ACCTGC ACCTGC(4/8)
194 GCAGGT
BstYI 30 GGATCT [GA]^GATC[CT]
107 GGATCT
Cac8I 80 GCCAGC GC[ACGT]^[ACGT]GC
512 GCCAGC
HphI 98 TCACC GGTGA(8/7)
167 TCACC
MflI 30 GGATCT [GA]^GATC[CT]
107 GGATCT
MspA1I 57 CAGCGG C[AC]G^C[GT]G
103 CCGCGG
NciI 354 CCGGG CC^[GC]GG
372 CCGGG
SfcI 66 CTACAG C^T[GA][CT]AG
151 CTGCAG
XhoII 30 GGATCT [GA]^GATC[CT]
107 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AluI BbvI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MaeI MaeII MboI
MnlI MspI MvaI MvnI MwoI
NdeII NlaIII Sau3AI Sau96I ScrFI
SfaNI TseI