Restriction map for
pFN21AE1530
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BamHI
BbeI BbrPI BbsI BbvCI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BsiWI
BsmI Bsp1407I BspEI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I DraI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KasI KpnI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 145 GTCTAC GT^[AC][GT]AC
AcyI 883 GACGCC G[GA]^CG[CT]C
Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 749 TGCGCA TGC^GCA
BcgI 1013 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1255 GTATCC GTATCC(6/5)
BclI 68 TGATCA T^GATCA
BpmI 130 CTGGAG CTGGAG(16/14)
BsaAI 106 TACGTG [CT]AC^GT[GA]
BsaHI 883 GACGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsmBI 164 CGTCTC CGTCTC(1/5)
BsmFI 668 GGGAC GGGAC(10/14)
BspDI 1330 ATCGAT AT^CGAT
BspHI 488 TCATGA T^CATGA
BspMI 746 ACCTGC ACCTGC(4/8)
BsrBI 814 CCGCTC CCGCTC(-3/-3)
BstEII 705 GGTCACC G^GT[ACGT]ACC
BstPI 705 GGTCACC G^GT[ACGT]ACC
BstYI 461 AGATCC [GA]^GATC[CT]
ClaI 1330 ATCGAT AT^CGAT
CpoI 479 CGGACCG CG^G[AT]CCG
DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 95 CGGCCG C^GGCCG
EarI 1321 GAAGAG CTCTTC(1/4)
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoO65I 705 GGTCACC G^GT[ACGT]ACC
FbaI 68 TGATCA T^GATCA
FspI 749 TGCGCA TGC^GCA
Hin1I 883 GACGCC G[GA]^CG[CT]C
HindIII 1304 AAGCTT A^AGCTT
Ksp632I 1321 GAAGAG CTCTTC(1/4)
MaeIII 706 GTCAC ^GT[ACGT]AC
MflI 461 AGATCC [GA]^GATC[CT]
PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 488 TCATGA T^CATGA
RsrII 479 CGGACCG CG^G[AT]CCG
SacI 862 GAGCTC GAGCT^C
SbfI 387 CCTGCAGG CCTGCA^GG
SmaI 726 CCCGGG CCC^GGG
Sse8387I 387 CCTGCAGG CCTGCA^GG
StuI 1175 AGGCCT AGG^CCT
Tsp45I 706 GTCAC ^GT[GC]AC
XhoII 461 AGATCC [GA]^GATC[CT]
XmaCI 726 CCCGGG C^CCGGG
XmaI 726 CCCGGG C^CCGGG
XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 13 GTGCAC G^TGCAC
565 GTGCAC
AlwI 462 GATCC GGATC(4/5)
910 GATCC
AlwNI 985 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGGAGCTG
ApaI 845 GGGCCC GGGCC^C
955 GGGCCC
ApaLI 13 GTGCAC G^TGCAC
565 GTGCAC
AvaI 726 CCCGGG C^[CT]CG[GA]G
859 CCCGAG
AvaII 480 GGACC G^G[AT]CC
669 GGACC
BglI 237 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
386 GCCTGCAGGGC
BseRI 469 GAGGAG GAGGAG(10/8)
1324 GAGGAG
BsoBI 726 CCCGGG C^[CT]CG[GA]G
859 CCCGAG
DraII 668 GGGACCT [GA]G^G[ACGT]CC[CT]
845 GGGCCCT
EcoO109I 668 GGGACCT [GA]G^G[ACGT]CC[CT]
845 GGGCCCT
HaeII 120 GGCGCT [GA]GCGC^[CT]
615 GGCGCT
MslI 531 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
960 CATGTCCATG
NcoI 215 CCATGG C^CATGG
918 CCATGG
PflMI 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGCTGGTGG
PleI 115 GACTC GAGTC(4/5)
157 GAGTC
PstI 124 CTGCAG CTGCA^G
388 CTGCAG
PvuII 127 CAGCTG CAG^CTG
571 CAGCTG
SexAI 680 ACCTGGT A^CC[AT]GGT
1250 ACCTGGT
SfcI 124 CTGCAG C^T[GA][CT]AG
388 CTGCAG
TfiI 137 GAATC G^A[AT]TC
1032 GATTC
Van91I 53 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BalI BanI BanII BbvI BcnI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TspRI
TthHB8I XcmI