Restriction map for
pFN21AE1531
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Aor51HI ApaI AscI
Asp700I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbvCI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BseAI BseRI BsgI BsiEI BsoBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
ClaI CpoI DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI FspI HaeII HhaI HinP1I
HindIII KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 352 GGTACC G^GTACC
AccII 1168 CGCG CG^CG
Alw44I 240 GTGCAC G^TGCAC
AocI 1047 CCTTAGG CC^T[ACGT]AGG
ApaLI 240 GTGCAC G^TGCAC
AseI 811 ATTAAT AT^TAAT
AsnI 811 ATTAAT AT^TAAT
Asp718I 352 GGTACC G^GTACC
BbrPI 289 CACGTG CAC^GTG
BbsI 1117 GAAGAC GAAGAC(2/6)
BcgI 1016 GCATCGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1052 GGATAC GTATCC(6/5)
BpuAI 1117 GAAGAC GAAGAC(2/6)
BsaAI 289 CACGTG [CT]AC^GT[GA]
BsaWI 488 TCCGGT [AT]^CCGG[AT]
BsmBI 560 CGTCTC CGTCTC(1/5)
BsmFI 146 GGGAC GGGAC(10/14)
BsmI 49 GCATTC GAATGC(1/-1)
BsrDI 665 GCAATG GCAATG(2/0)
BstUI 1168 CGCG CG^CG
Bsu36I 1047 CCTTAGG CC^T[ACGT]AGG
DraI 1036 TTTAAA TTT^AAA
DraII 721 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 874 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1171 GAAGAG CTCTTC(1/4)
Eco81I 1047 CCTTAGG CC^T[ACGT]AGG
EcoNI 410 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 721 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1138 GAATTC G^AATTC
FauI 1068 CCCGC CCCGC(4/6)
HaeIII 429 GGCC GG^CC
HapII 489 CCGG C^CGG
HgaI 690 GACGC GACGC(5/10)
HincII 580 GTTAAC GT[CT]^[GA]AC
HindII 580 GTTAAC GT[CT]^[GA]AC
HpaI 580 GTTAAC GTT^AAC
HpaII 489 CCGG C^CGG
KpnI 352 GGTACC GGTAC^C
Ksp632I 1171 GAAGAG CTCTTC(1/4)
MslI 978 CATTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 489 CCGG C^CGG
MvnI 1168 CGCG CG^CG
PflMI 1107 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 289 CACGTG CAC^GTG
PmlI 289 CACGTG CAC^GTG
PpuMI 721 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 811 ATTAAT AT^TAAT
PstI 893 CTGCAG CTGCA^G
PvuII 760 CAGCTG CAG^CTG
SacI 918 GAGCTC GAGCT^C
SfcI 893 CTGCAG C^T[GA][CT]AG
SpeI 298 ACTAGT A^CTAGT
Van91I 1107 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 421 GGTCC G^G[AT]CC
722 GGACC
BanI 90 GGCACC G^G[CT][GA]CC
352 GGTACC
BanII 859 GAGCCC G[GA]GC[CT]^C
918 GAGCTC
BbvI 358 GCTGC GCAGC(8/12)
892 GCTGC
BfaI 101 CTAG C^TAG
299 CTAG
BpmI 123 CTCCAG CTGGAG(16/14)
924 CTGGAG
BsiWI 675 CGTACG C^GTACG
702 CGTACG
BsmAI 104 GTCTC GTCTC(1/5)
561 GTCTC
Bsp1407I 455 TGTACA T^GTACA
974 TGTACA
BsrGI 455 TGTACA T^GTACA
974 TGTACA
BsrI 444 CCAGT ACTGG(1/-1)
1072 CCAGT
BstF5I 606 CATCC GGATG(2/0)
529 GGATG
BstYI 75 GGATCT [GA]^GATC[CT]
485 AGATCC
FokI 606 CATCC GGATG(9/13)
529 GGATG
MaeI 101 CTAG C^TAG
299 CTAG
MflI 75 GGATCT [GA]^GATC[CT]
485 AGATCC
MwoI 143 GCTGGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1062 GCTGTTCCCGC
SspBI 455 TGTACA T^GTACA
974 TGTACA
TfiI 619 GATTC G^A[AT]TC
650 GATTC
TseI 358 GCTGC G^C[AT]GC
892 GCTGC
XhoII 75 GGATCT [GA]^GATC[CT]
485 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI Hin1I
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
Tru9I Tsp45I Tsp509I TspRI