Restriction map for
pFN21AE5105
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssKI Bst1107I BstAPI BstEII
BstF5I BstNI BstPI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DdeI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FokI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvaI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI ScrFI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 43 CGCG CG^CG
AcsI 112 AAATTC [GA]^AATT[CT]
ApoI 112 AAATTC [GA]^AATT[CT]
AvaII 279 GGACC G^G[AT]CC
BbrPI 49 CACGTG CAC^GTG
BbsI 172 GTCTTC GAAGAC(2/6)
BcgI 54 GCAGCCTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 172 GTCTTC GAAGAC(2/6)
BsaAI 49 CACGTG [CT]AC^GT[GA]
BspDI 257 ATCGAT AT^CGAT
BspMI 306 ACCTGC ACCTGC(4/8)
BssSI 263 CTCGTG CACGAG(-5/-1)
BstBI 122 TTCGAA TT^CGAA
BstUI 43 CGCG CG^CG
ClaI 257 ATCGAT AT^CGAT
DpnII 260 GATC ^GATC
DsaI 42 CCGCGG C^C[GA][CT]GG
EaeI 45 CGGCCA [CT]^GGCC[GA]
EcoT14I 92 CCAAGG C^C[AT][AT]GG
HgaI 170 GCGTC GACGC(5/10)
HinfI 255 GAATC G^A[ACGT]TC
KspI 42 CCGCGG CCGC^GG
MboI 260 GATC ^GATC
MboII 173 TCTTC GAAGA(8/7)
MnlI 136 GAGG CCTC(7/6)
MseI 107 TTAA T^TAA
MslI 214 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 42 CCGCGG C[AC]G^C[GT]G
MvnI 43 CGCG CG^CG
NdeII 260 GATC ^GATC
NlaIII 342 CATG CATG^
NspV 122 TTCGAA TT^CGAA
PmaCI 49 CACGTG CAC^GTG
PmlI 49 CACGTG CAC^GTG
SacII 42 CCGCGG CCGC^GG
Sau3AI 260 GATC ^GATC
SfuI 122 TTCGAA TT^CGAA
StyI 92 CCAAGG C^C[AT][AT]GG
TfiI 255 GAATC G^A[AT]TC
Tru9I 107 TTAA T^TAA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 197 AGCT AG^CT
209 AGCT
BmyI 77 GTGCCC G[AGT]GC[ACT]^C
88 GTGCCC
BsaJI 42 CCGCGG C^C[ACGT][ACGT]GG
92 CCAAGG
BsgI 66 CTGCAC GTGCAG(16/14)
52 GTGCAG
BsmFI 152 GGGAC GGGAC(10/14)
326 GTCCC
Bsp1286I 77 GTGCCC G[AGT]GC[ACT]^C
88 GTGCCC
Cfr13I 39 GGGCC G^G[ACGT]CC
279 GGACC
FauI 272 CCCGC CCCGC(4/6)
310 GCGGG
MaeII 50 ACGT A^CGT
324 ACGT
MwoI 189 GCTTCCCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
310 GCGGGTGCTGC
Sau96I 39 GGGCC G^G[ACGT]CC
279 GGACC
SfaNI 83 GATGC GCATC(5/9)
178 GATGC
Tsp509I 113 AATT ^AATT
234 AATT
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BbvI Cac8I Fnu4HI HaeIII
ItaI TaqI TseI TthHB8I