Restriction map for
pFN21AE6215
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BanI BbeI BbrPI BbsI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HapII HgaI Hin1I
HincII HindII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MspA1I MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 698 CCGC CCGC(-3/-1)
Aor51HI 459 AGCGCT AGC^GCT
BamHI 48 GGATCC G^GATCC
BanII 732 GAGCCC G[GA]GC[CT]^C
BbvCI 401 CCTCAGC CCTCAGC(-5/-2)
BciVI 336 GGATAC GTATCC(6/5)
BfaI 316 CTAG C^TAG
BmyI 732 GAGCCC G[AGT]GC[ACT]^C
BpmI 775 CTCCAG CTGGAG(16/14)
BsaI 254 GGTCTC GGTCTC(1/5)
BsgI 720 CTGCAC GTGCAG(16/14)
BsmI 273 GAATGC GAATGC(1/-1)
Bsp1286I 732 GAGCCC G[AGT]GC[ACT]^C
BspDI 646 ATCGAT AT^CGAT
BssHII 689 GCGCGC G^CGCGC
BstYI 48 GGATCC [GA]^GATC[CT]
ClaI 646 ATCGAT AT^CGAT
DraII 704 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eco47III 459 AGCGCT AGC^GCT
EcoO109I 704 AGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 459 AGCGCT [GA]GCGC^[CT]
HphI 637 GGTGA GGTGA(8/7)
MaeI 316 CTAG C^TAG
MaeIII 638 GTGAC ^GT[ACGT]AC
MflI 48 GGATCC [GA]^GATC[CT]
MseI 656 TTAA T^TAA
MslI 492 CATAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PstI 725 CTGCAG CTGCA^G
SfcI 725 CTGCAG C^T[GA][CT]AG
TaqI 647 TCGA T^CGA
TfiI 524 GAATC G^A[AT]TC
Tru9I 656 TTAA T^TAA
Tsp45I 638 GTGAC ^GT[GC]AC
TthHB8I 647 TCGA T^CGA
XhoII 48 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 688 CGCG CG^CG
690 CGCG
BalI 42 TGGCCA TGG^CCA
752 TGGCCA
BsmFI 171 GGGAC GGGAC(10/14)
590 GTCCC
BsrI 757 ACTGG ACTGG(1/-1)
131 CCAGT
BstF5I 154 CATCC GGATG(2/0)
21 GGATG
BstUI 688 CGCG CG^CG
690 CGCG
BstXI 191 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
777 CCAGCCCCATGG
Cfr13I 673 GGCCC G^G[ACGT]CC
705 GGGCC
DdeI 140 CTGAG C^T[ACGT]AG
402 CTCAG
DsaI 482 CCATGG C^C[GA][CT]GG
783 CCATGG
EarI 125 CTCTTC CTCTTC(1/4)
413 CTCTTC
FokI 154 CATCC GGATG(9/13)
21 GGATG
HindIII 145 AAGCTT A^AGCTT
370 AAGCTT
Ksp632I 125 CTCTTC CTCTTC(1/4)
413 CTCTTC
MluNI 42 TGGCCA TGG^CCA
752 TGGCCA
MscI 42 TGGCCA TGG^CCA
752 TGGCCA
MvnI 688 CGCG CG^CG
690 CGCG
NcoI 482 CCATGG C^CATGG
783 CCATGG
NlaIII 483 CATG CATG^
784 CATG
PleI 173 GACTC GAGTC(4/5)
397 GAGTC
Sau96I 673 GGCCC G^G[ACGT]CC
705 GGGCC
SmlI 463 CTCAAG C^T[CT][GA]AG
613 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BsmAI BssKI BstNI Cac8I
CfoI DpnII EaeI EcoRII EcoT14I
Fnu4HI HaeIII HhaI HinP1I HinfI
ItaI MboI MboII MnlI MvaI
MwoI NdeII NlaIV Sau3AI ScrFI
SfaNI StyI TseI Tsp509I TspRI