Restriction map for
pFN21AE6123
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr13I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 238 GGCGTC G[GA]^CG[CT]C
AfaI 433 GTAC GT^AC
AgeI 60 ACCGGT A^CCGGT
BanII 117 GAGCCC G[GA]GC[CT]^C
BmyI 117 GAGCCC G[AGT]GC[ACT]^C
BsaHI 238 GGCGTC G[GA]^CG[CT]C
BsaWI 60 ACCGGT [AT]^CCGG[AT]
BsgI 64 GTGCAG GTGCAG(16/14)
BsiYI 24 CCTGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 24 CCTGCTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 410 GTCTC GTCTC(1/5)
BsmFI 241 GTCCC GGGAC(10/14)
Bsp1286I 117 GAGCCC G[AGT]GC[ACT]^C
BsrFI 60 ACCGGT [GA]^CCGG[CT]
Cac8I 140 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 34 GCGC GCG^C
Cfr10I 60 ACCGGT [GA]^CCGG[CT]
DpnII 438 GATC ^GATC
DsaI 215 CCGTGG C^C[GA][CT]GG
EarI 412 CTCTTC CTCTTC(1/4)
HaeII 33 GGCGCT [GA]GCGC^[CT]
HapII 61 CCGG C^CGG
HhaI 34 GCGC GCG^C
Hin1I 238 GGCGTC G[GA]^CG[CT]C
HinP1I 34 GCGC G^CGC
HpaII 61 CCGG C^CGG
Ksp632I 412 CTCTTC CTCTTC(1/4)
MaeIII 175 GTCAC ^GT[ACGT]AC
MboI 438 GATC ^GATC
MboII 413 TCTTC GAAGA(8/7)
MnlI 359 CCTC CCTC(7/6)
MseI 290 TTAA T^TAA
MslI 228 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 61 CCGG C^CGG
NdeII 438 GATC ^GATC
PinAI 60 ACCGGT A^CCGGT
PvuII 142 CAGCTG CAG^CTG
RsaI 433 GTAC GT^AC
Sau3AI 438 GATC ^GATC
SfaNI 227 GCATC GCATC(5/9)
SfcI 318 CTATAG C^T[GA][CT]AG
SspI 190 AATATT AAT^ATT
Tru9I 290 TTAA T^TAA
Tsp45I 175 GTCAC ^GT[GC]AC
Tsp509I 288 AATT ^AATT
TspRI 341 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AciI 44 CCGC CCGC(-3/-1)
80 GCGG
BlpI 36 GCTCAGC GC^T[ACGT]AGC
144 GCTGAGC
Bpu1102I 36 GCTCAGC GC^T[ACGT]AGC
144 GCTGAGC
BsaJI 50 CCCAGG C^C[ACGT][ACGT]GG
215 CCGTGG
CelII 36 GCTCAGC GC^T[ACGT]AGC
144 GCTGAGC
HaeIII 54 GGCC GG^CC
213 GGCC
HgaI 167 GCGTC GACGC(5/10)
239 GCGTC
HphI 176 TCACC GGTGA(8/7)
182 TCACC
MspA1I 44 CCGCTG C[AC]G^C[GT]G
142 CAGCTG
NlaIII 122 CATG CATG^
223 CATG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsrI BssKI BstF5I
BstNI DdeI EcoRII Fnu4HI FokI
ItaI MvaI MwoI ScrFI TseI