Restriction map for
pFN21AE1375
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII HphI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI MwoI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 507 GTAC GT^AC
AlwI 348 GATCC GGATC(4/5)
AlwNI 124 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 323 GAGCAC G[AT]GC[AT]^C
BbsI 442 GTCTTC GAAGAC(2/6)
BciVI 78 GTATCC GTATCC(6/5)
BpuAI 442 GTCTTC GAAGAC(2/6)
BsaJI 47 CCATGG C^C[ACGT][ACGT]GG
BsiHKAI 323 GAGCAC G[AT]GC[AT]^C
BsmFI 234 GTCCC GGGAC(10/14)
BssKI 166 CCAGG ^CC[ACGT]GG
BssSI 320 CACGAG CACGAG(-5/-1)
BstNI 166 CCAGG CC^[AT]GG
BstXI 41 CCACATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 347 AGATCC [GA]^GATC[CT]
DraI 544 TTTAAA TTT^AAA
DsaI 47 CCATGG C^C[GA][CT]GG
EcoRI 295 GAATTC G^AATTC
EcoRII 166 CCAGG ^CC[AT]GG
EcoT14I 47 CCATGG C^C[AT][AT]GG
HaeIII 633 GGCC GG^CC
HincII 220 GTTAAC GT[CT]^[GA]AC
HindII 220 GTTAAC GT[CT]^[GA]AC
HinfI 232 GAGTC G^A[ACGT]TC
HpaI 220 GTTAAC GTT^AAC
MaeII 538 ACGT A^CGT
MflI 347 AGATCC [GA]^GATC[CT]
MspA1I 251 CAGCTG C[AC]G^C[GT]G
MvaI 166 CCAGG CC^[AT]GG
NcoI 47 CCATGG C^CATGG
PleI 232 GAGTC GAGTC(4/5)
PvuII 251 CAGCTG CAG^CTG
RsaI 507 GTAC GT^AC
ScaI 506 AGTACT AGT^ACT
ScrFI 166 CCAGG CC^[ACGT]GG
StyI 47 CCATGG C^C[AT][AT]GG
XhoII 347 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 137 CCGC CCGC(-3/-1)
468 CCGC
BbvI 118 GCAGC GCAGC(8/12)
250 GCAGC
BfaI 97 CTAG C^TAG
404 CTAG
BmyI 323 GAGCAC G[AGT]GC[ACT]^C
473 GGGCAC
BsaI 37 GAGACC GGTCTC(1/5)
169 GGTCTC
BsmAI 37 GAGAC GTCTC(1/5)
170 GTCTC
Bsp1286I 323 GAGCAC G[AGT]GC[ACT]^C
473 GGGCAC
BstF5I 44 CATCC GGATG(2/0)
5 GGATG
DpnII 348 GATC ^GATC
360 GATC
EarI 16 GAAGAG CTCTTC(1/4)
265 GAAGAG
FokI 44 CATCC GGATG(9/13)
5 GGATG
Ksp632I 16 GAAGAG CTCTTC(1/4)
265 GAAGAG
MaeI 97 CTAG C^TAG
404 CTAG
MboI 348 GATC ^GATC
360 GATC
MslI 42 CACATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
183 CATCATTATG
NdeII 348 GATC ^GATC
360 GATC
NlaIII 48 CATG CATG^
75 CATG
PstI 116 CTGCAG CTGCA^G
382 CTGCAG
Sau3AI 348 GATC ^GATC
360 GATC
SfcI 116 CTGCAG C^T[GA][CT]AG
382 CTGCAG
TseI 118 GCAGC G^C[AT]GC
250 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AcsI AluI ApoI DdeI Fnu4HI
ItaI MboII MnlI MseI Tru9I
Tsp509I TspRI