Restriction map for
pFN21AE1509
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AfaI 270 GTAC GT^AC
AocI 672 CCTGAGG CC^T[ACGT]AGG
AspHI 1008 GAGCTC G[AT]GC[AT]^C
AvaI 39 CTCGGG C^[CT]CG[GA]G
AvrII 969 CCTAGG C^CTAGG
BlnI 969 CCTAGG C^CTAGG
BseRI 397 CTCCTC GAGGAG(10/8)
BsgI 853 GTGCAG GTGCAG(16/14)
BsiEI 117 CGGCCG CG[GA][CT]^CG
BsiHKAI 1008 GAGCTC G[AT]GC[AT]^C
BsoBI 39 CTCGGG C^[CT]CG[GA]G
BssHII 612 GCGCGC G^CGCGC
BssSI 859 CACGAG CACGAG(-5/-1)
BstAPI 165 GCAGTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 672 CCTGAGG CC^T[ACGT]AGG
EaeI 117 CGGCCG [CT]^GGCC[GA]
EagI 117 CGGCCG C^GGCCG
EclXI 117 CGGCCG C^GGCCG
Eco52I 117 CGGCCG C^GGCCG
Eco81I 672 CCTGAGG CC^T[ACGT]AGG
EcoNI 447 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 969 CCTAGG C^C[AT][AT]GG
MaeIII 637 GTGAC ^GT[ACGT]AC
PpuMI 455 AGGACCT [GA]G^G[AT]CC[CT]
PstI 607 CTGCAG CTGCA^G
PvuII 806 CAGCTG CAG^CTG
RsaI 270 GTAC GT^AC
SacI 1008 GAGCTC GAGCT^C
SfaNI 929 GCATC GCATC(5/9)
SfcI 607 CTGCAG C^T[GA][CT]AG
StyI 969 CCTAGG C^C[AT][AT]GG
TaqI 179 TCGA T^CGA
Tsp45I 637 GTGAC ^GT[GC]AC
TthHB8I 179 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AflIII 633 ACACGT A^C[GA][CT]GT
842 ACACGT
ApaI 382 GGGCCC GGGCC^C
683 GGGCCC
BfaI 961 CTAG C^TAG
970 CTAG
BglI 384 GCCCGACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
520 GCCCGTGCGGC
BsmBI 651 CGTCTC CGTCTC(1/5)
601 GAGACG
BsmFI 251 GGGAC GGGAC(10/14)
108 GTCCC
BspMI 311 ACCTGC ACCTGC(4/8)
458 ACCTGC
BsrI 357 CCAGT ACTGG(1/-1)
414 CCAGT
DpnII 495 GATC ^GATC
708 GATC
EarI 829 GAAGAG CTCTTC(1/4)
1005 GAAGAG
HinfI 195 GAGTC G^A[ACGT]TC
1075 GAGTC
HphI 497 TCACC GGTGA(8/7)
881 TCACC
Ksp632I 829 GAAGAG CTCTTC(1/4)
1005 GAAGAG
MaeI 961 CTAG C^TAG
970 CTAG
MboI 495 GATC ^GATC
708 GATC
NdeII 495 GATC ^GATC
708 GATC
PleI 195 GAGTC GAGTC(4/5)
1075 GAGTC
SapI 829 GAAGAGC GCTCTTC(1/4)
1005 GAAGAGC
Sau3AI 495 GATC ^GATC
708 GATC
XcmI 357 CCAGTGGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
914 CCATCCCCATCGTGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbeI BbrPI BbvI
BcnI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MaeII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PmaCI PmlI SacII Sau96I ScrFI
SfoI TseI TspRI