Restriction map for
pFN21AE4487
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BcgI BclI BcnI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
Cac8I CelII ClaI CpoI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MroI MslI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 1159 GACGTC GACGT^C
AcyI 1159 GACGTC G[GA]^CG[CT]C
AseI 487 ATTAAT AT^TAAT
AsnI 487 ATTAAT AT^TAAT
Asp700I 1368 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 711 TGGCCA TGG^CCA
BbvCI 1458 GCTGAGG CCTCAGC(-5/-2)
BciVI 869 GGATAC GTATCC(6/5)
BglII 434 AGATCT A^GATCT
BpmI 79 CTGGAG CTGGAG(16/14)
BsaBI 958 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1159 GACGTC G[GA]^CG[CT]C
BsaI 1393 GGTCTC GGTCTC(1/5)
BsgI 100 GTGCAG GTGCAG(16/14)
BsmFI 1268 GTCCC GGGAC(10/14)
Bsp1407I 167 TGTACA T^GTACA
BspMI 718 ACCTGC ACCTGC(4/8)
BsrFI 44 GCCGGT [GA]^CCGG[CT]
BsrGI 167 TGTACA T^GTACA
BstAPI 382 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 108 GCGC GCG^C
Cfr10I 44 GCCGGT [GA]^CCGG[CT]
DraI 1287 TTTAAA TTT^AAA
DsaI 509 CCACGG C^C[GA][CT]GG
EagI 42 CGGCCG C^GGCCG
EclXI 42 CGGCCG C^GGCCG
Eco52I 42 CGGCCG C^GGCCG
EcoRI 157 GAATTC G^AATTC
FauI 18 GCGGG CCCGC(4/6)
HhaI 108 GCGC GCG^C
Hin1I 1159 GACGTC G[GA]^CG[CT]C
HinP1I 108 GCGC G^CGC
HindIII 445 AAGCTT A^AGCTT
MamI 958 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 711 TGGCCA TGG^CCA
MscI 711 TGGCCA TGG^CCA
NruI 49 TCGCGA TCG^CGA
PshBI 487 ATTAAT AT^TAAT
PvuII 76 CAGCTG CAG^CTG
ScaI 948 AGTACT AGT^ACT
SmlI 214 CTTGAG C^T[CT][GA]AG
SspBI 167 TGTACA T^GTACA
StuI 281 AGGCCT AGG^CCT
TaqI 8 TCGA T^CGA
TfiI 219 GAATC G^A[AT]TC
TthHB8I 8 TCGA T^CGA
XmnI 1368 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 50 CGCG CG^CG
109 CGCG
AfaI 168 GTAC GT^AC
949 GTAC
BanII 4 GAGCTC G[GA]GC[CT]^C
514 GAGCTC
BseRI 1199 GAGGAG GAGGAG(10/8)
1461 GAGGAG
BsiEI 42 CGGCCG CG[GA][CT]^CG
46 CGGTCG
BsrDI 723 CATTGC GCAATG(2/0)
1154 GCAATG
BstUI 50 CGCG CG^CG
109 CGCG
BstXI 431 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1211 CCAGTGGGATGG
BstYI 434 AGATCT [GA]^GATC[CT]
471 GGATCT
DraII 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
1469 AGGCCCT
EcoO109I 83 AGGCCCT [GA]G^G[ACGT]CC[CT]
1469 AGGCCCT
EcoRV 118 GATATC GAT^ATC
1316 GATATC
EcoT14I 192 CCTTGG C^C[AT][AT]GG
974 CCAAGG
HgaI 680 GACGC GACGC(5/10)
1266 GCGTC
MflI 434 AGATCT [GA]^GATC[CT]
471 GGATCT
MspA1I 76 CAGCTG C[AC]G^C[GT]G
1427 CCGCTG
MvnI 50 CGCG CG^CG
109 CGCG
PleI 612 GACTC GAGTC(4/5)
823 GAGTC
PstI 70 CTGCAG CTGCA^G
380 CTGCAG
RsaI 168 GTAC GT^AC
949 GTAC
SacI 4 GAGCTC GAGCT^C
514 GAGCTC
SfcI 70 CTGCAG C^T[GA][CT]AG
380 CTGCAG
StyI 192 CCTTGG C^C[AT][AT]GG
974 CCAAGG
XhoII 434 AGATCT [GA]^GATC[CT]
471 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AspHI AvaII BbvI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I Tsp509I TspRI