################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII BalI BanII BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmI BspDI BspEI BspLU11I BsrDI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 1092 GTATAC GT^[AC][GT]AC AcyI 104 GGCGCC G[GA]^CG[CT]C AflIII 546 ACGTGT A^C[GA][CT]GT AocI 933 CCTGAGG CC^T[ACGT]AGG Asp700I 989 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 647 CTCGGG C^[CT]CG[GA]G AvrII 67 CCTAGG C^CTAGG BamHI 899 GGATCC G^GATCC BanI 104 GGCGCC G^G[CT][GA]CC BbeI 104 GGCGCC GGCGC^C BclI 972 TGATCA T^GATCA BcnI 903 CCCGG CC^[GC]GG BglI 470 GCCTTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 67 CCTAGG C^CTAGG BpmI 242 CTGGAG CTGGAG(16/14) BsaHI 104 GGCGCC G[GA]^CG[CT]C BsaWI 977 ACCGGA [AT]^CCGG[AT] BseRI 1142 CTCCTC GAGGAG(10/8) BsgI 964 GTGCAG GTGCAG(16/14) BsmAI 780 GAGAC GTCTC(1/5) BsmBI 780 GAGACG CGTCTC(1/5) BsoBI 647 CTCGGG C^[CT]CG[GA]G Bsp1407I 485 TGTACA T^GTACA BspHI 402 TCATGA T^CATGA BspMI 802 GCAGGT ACCTGC(4/8) BsrGI 485 TGTACA T^GTACA Bst1107I 1092 GTATAC GTA^TAC BstYI 899 GGATCC [GA]^GATC[CT] BstZ17I 1092 GTATAC GTA^TAC Bsu36I 933 CCTGAGG CC^T[ACGT]AGG DraI 388 TTTAAA TTT^AAA DraIII 86 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 717 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1148 TGGCCG [CT]^GGCC[GA] EarI 1217 GAAGAG CTCTTC(1/4) Eco81I 933 CCTGAGG CC^T[ACGT]AGG FauI 198 GCGGG CCCGC(4/6) FbaI 972 TGATCA T^GATCA Hin1I 104 GGCGCC G[GA]^CG[CT]C HincII 273 GTTGAC GT[CT]^[GA]AC HindII 273 GTTGAC GT[CT]^[GA]AC KasI 104 GGCGCC G^GCGCC Ksp632I 1217 GAAGAG CTCTTC(1/4) MaeII 546 ACGT A^CGT MflI 899 GGATCC [GA]^GATC[CT] NarI 104 GGCGCC GG^CGCC NciI 903 CCCGG CC^[GC]GG NcoI 959 CCATGG C^CATGG PflFI 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 707 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 908 AGGACCT [GA]G^G[AT]CC[CT] PstI 225 CTGCAG CTGCA^G RcaI 402 TCATGA T^CATGA ScaI 446 AGTACT AGT^ACT SfoI 104 GGCGCC GGC^GCC SspBI 485 TGTACA T^GTACA StuI 477 AGGCCT AGG^CCT TaqI 292 TCGA T^CGA Tth111I 276 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 292 TCGA T^CGA Van91I 707 CCAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 899 GGATCC [GA]^GATC[CT] XmnI 989 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AluI 147 AGCT AG^CT 709 AGCT BlpI 193 GCTGAGC GC^T[ACGT]AGC 525 GCTCAGC Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC 525 GCTCAGC BsrBI 55 CCGCTC CCGCTC(-3/-3) 196 GAGCGG BsrI 1186 ACTGG ACTGG(1/-1) 1161 CCAGT CelII 193 GCTGAGC GC^T[ACGT]AGC 525 GCTCAGC CfoI 76 GCGC GCG^C 105 GCGC DraII 28 AGGGCCT [GA]G^G[ACGT]CC[CT] 908 AGGACCT DsaI 531 CCGTGG C^C[GA][CT]GG 959 CCATGG EcoO109I 28 AGGGCCT [GA]G^G[ACGT]CC[CT] 908 AGGACCT HaeII 75 AGCGCC [GA]GCGC^[CT] 104 GGCGCC HapII 904 CCGG C^CGG 978 CCGG HhaI 76 GCGC GCG^C 105 GCGC HinP1I 76 GCGC G^CGC 105 GCGC HpaII 904 CCGG C^CGG 978 CCGG MboII 619 GAAGA GAAGA(8/7) 1217 GAAGA MseI 310 TTAA T^TAA 389 TTAA MslI 957 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1008 CATTACAGTG MspI 904 CCGG C^CGG 978 CCGG SfcI 225 CTGCAG C^T[GA][CT]AG 772 CTACAG Tru9I 310 TTAA T^TAA 389 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AcsI AfaI AlwI AlwNI ApoI AspHI AvaII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstAPI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp45I Tsp509I TspRI