Restriction map for
pFN21AE4329
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BglI BglII
BlnI BmyI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HpaI HphI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 472 CGCG CG^CG
AcsI 355 AAATTC [GA]^AATT[CT]
AfaI 381 GTAC GT^AC
AflII 10 CTTAAG C^TTAAG
AlwI 192 GGATC GGATC(4/5)
AlwNI 549 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 355 AAATTC [GA]^AATT[CT]
AvaI 893 CCCGGG C^[CT]CG[GA]G
AvaII 987 GGACC G^G[AT]CC
BciVI 881 GTATCC GTATCC(6/5)
BfaI 506 CTAG C^TAG
BfrI 10 CTTAAG C^TTAAG
BlpI 943 GCTCAGC GC^T[ACGT]AGC
BpmI 634 CTCCAG CTGGAG(16/14)
Bpu1102I 943 GCTCAGC GC^T[ACGT]AGC
BsaAI 432 TACGTA [CT]AC^GT[GA]
BsiYI 649 CCAGAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 649 CCAGAAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 129 GTCTC GTCTC(1/5)
BsmFI 974 GGGAC GGGAC(10/14)
BsoBI 893 CCCGGG C^[CT]CG[GA]G
BspMI 622 GCAGGT ACCTGC(4/8)
BsrDI 24 CATTGC GCAATG(2/0)
BstAPI 28 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 472 CGCG CG^CG
BstXI 413 CCAACCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 192 GGATCT [GA]^GATC[CT]
CelII 943 GCTCAGC GC^T[ACGT]AGC
Cfr13I 987 GGACC G^G[ACGT]CC
DraII 986 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 469 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 558 CCATGG C^C[GA][CT]GG
EarI 43 GAAGAG CTCTTC(1/4)
EcoO109I 986 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 558 CCATGG C^C[AT][AT]GG
HapII 894 CCGG C^CGG
HincII 822 GTCAAC GT[CT]^[GA]AC
HindII 822 GTCAAC GT[CT]^[GA]AC
HindIII 172 AAGCTT A^AGCTT
HpaII 894 CCGG C^CGG
Ksp632I 43 GAAGAG CTCTTC(1/4)
MaeI 506 CTAG C^TAG
MaeII 433 ACGT A^CGT
MflI 192 GGATCT [GA]^GATC[CT]
MspA1I 552 CAGCTG C[AC]G^C[GT]G
MspI 894 CCGG C^CGG
MvnI 472 CGCG CG^CG
MwoI 28 GCACCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 558 CCATGG C^CATGG
PpuMI 986 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 552 CAGCTG CAG^CTG
RsaI 381 GTAC GT^AC
Sau96I 987 GGACC G^G[ACGT]CC
SfaNI 395 GCATC GCATC(5/9)
SmaI 893 CCCGGG CCC^GGG
SnaBI 432 TACGTA TAC^GTA
StyI 558 CCATGG C^C[AT][AT]GG
Tsp45I 256 GTGAC ^GT[GC]AC
XhoII 192 GGATCT [GA]^GATC[CT]
XmaCI 893 CCCGGG C^CCGGG
XmaI 893 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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BbsI 349 GTCTTC GAAGAC(2/6)
862 GTCTTC
BcnI 893 CCCGG CC^[GC]GG
894 CCGGG
BpuAI 349 GTCTTC GAAGAC(2/6)
862 GTCTTC
BsgI 331 GTGCAG GTGCAG(16/14)
959 GTGCAG
BsmI 116 GCATTC GAATGC(1/-1)
539 GCATTC
MslI 72 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
399 CATAATTGTG
NciI 893 CCCGG CC^[GC]GG
894 CCGGG
PstI 196 CTGCAG CTGCA^G
287 CTGCAG
TspRI 72 CACTG CA[GC]TG[ACGT][ACGT]^
419 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII PleI Sau3AI
ScrFI SfcI SmlI TfiI Tru9I
TseI Tsp509I