Restriction map for
pFN21AE4464
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BanI
BbeI BbrPI BcgI BciVI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsmBI
BsmI Bsp1407I BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstXI
Bsu36I Cfr10I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 77 enzymes) #
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AccII 1355 CGCG CG^CG
AciI 739 CCGC CCGC(-3/-1)
AflIII 51 ACATGT A^C[GA][CT]GT
AhdI 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 610 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1327 GTGCTC G[AT]GC[AT]^C
AspI 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 612 GGACC G^G[AT]CC
BalI 593 TGGCCA TGG^CCA
BamHI 940 GGATCC G^GATCC
BbsI 242 GAAGAC GAAGAC(2/6)
BbvCI 703 GCTGAGG CCTCAGC(-5/-2)
BclI 96 TGATCA T^GATCA
BlpI 1023 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1023 GCTTAGC GC^T[ACGT]AGC
BpuAI 242 GAAGAC GAAGAC(2/6)
BsaAI 1257 CACGTA [CT]AC^GT[GA]
BseRI 982 GAGGAG GAGGAG(10/8)
BsiHKAI 1327 GTGCTC G[AT]GC[AT]^C
BsiWI 1414 CGTACG C^GTACG
BsmFI 227 GGGAC GGGAC(10/14)
BspHI 99 TCATGA T^CATGA
BspLU11I 51 ACATGT A^CATGT
BspMI 1196 ACCTGC ACCTGC(4/8)
BsrDI 170 GCAATG GCAATG(2/0)
Bst1107I 639 GTATAC GTA^TAC
BstBI 842 TTCGAA TT^CGAA
BstEII 894 GGTTACC G^GT[ACGT]ACC
BstPI 894 GGTTACC G^GT[ACGT]ACC
BstUI 1355 CGCG CG^CG
BstYI 940 GGATCC [GA]^GATC[CT]
BstZ17I 639 GTATAC GTA^TAC
CelII 1023 GCTTAGC GC^T[ACGT]AGC
CfoI 599 GCGC GCG^C
DraI 1249 TTTAAA TTT^AAA
DraII 611 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 581 CCGTGG C^C[GA][CT]GG
EaeI 593 TGGCCA [CT]^GGCC[GA]
Eam1105I 145 GACAGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1222 GAAGAG CTCTTC(1/4)
EcoNI 870 CCTTATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 611 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 894 GGTTACC G^GT[ACGT]ACC
FauI 738 CCCGC CCCGC(4/6)
FbaI 96 TGATCA T^GATCA
HaeII 598 AGCGCC [GA]GCGC^[CT]
HhaI 599 GCGC GCG^C
HinP1I 599 GCGC G^CGC
HpaI 1336 GTTAAC GTT^AAC
Ksp632I 1222 GAAGAG CTCTTC(1/4)
MflI 940 GGATCC [GA]^GATC[CT]
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
MslI 69 CACGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1355 CGCG CG^CG
MwoI 1360 GCTAGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 1360 GCTAGC G^CTAGC
NruI 1354 TCGCGA TCG^CGA
NspI 51 ACATGT [GA]CATG^[CT]
NspV 842 TTCGAA TT^CGAA
PaeR7I 552 CTCGAG C^TCGAG
PflFI 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 400 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 611 AGGACCT [GA]G^G[AT]CC[CT]
PstI 451 CTGCAG CTGCA^G
PvuII 454 CAGCTG CAG^CTG
RcaI 99 TCATGA T^CATGA
SfcI 451 CTGCAG C^T[GA][CT]AG
SfuI 842 TTCGAA TT^CGAA
SmaI 1370 CCCGGG CCC^GGG
Tth111I 673 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 400 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 552 CTCGAG C^TCGAG
XhoII 940 GGATCC [GA]^GATC[CT]
XmaCI 1370 CCCGGG C^CCGGG
XmaI 1370 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 535 GTCTAC GT^[AC][GT]AC
639 GTATAC
AvaI 552 CTCGAG C^[CT]CG[GA]G
1370 CCCGGG
BanII 622 GGGCTC G[GA]GC[CT]^C
754 GAGCCC
BpmI 140 CTGGAG CTGGAG(16/14)
1106 CTGGAG
BsgI 547 CTGCAC GTGCAG(16/14)
607 GTGCAG
BsoBI 552 CTCGAG C^[CT]CG[GA]G
1370 CCCGGG
BspDI 529 ATCGAT AT^CGAT
1186 ATCGAT
Cfr13I 612 GGACC G^G[ACGT]CC
786 GGCCC
ClaI 529 ATCGAT AT^CGAT
1186 ATCGAT
HapII 1371 CCGG C^CGG
1448 CCGG
HgaI 245 GACGC GACGC(5/10)
1303 GACGC
HincII 106 GTTGAC GT[CT]^[GA]AC
1336 GTTAAC
HindII 106 GTTGAC GT[CT]^[GA]AC
1336 GTTAAC
HpaII 1371 CCGG C^CGG
1448 CCGG
HphI 175 GGTGA GGTGA(8/7)
585 GGTGA
MspA1I 454 CAGCTG C[AC]G^C[GT]G
739 CCGCTG
MspI 1371 CCGG C^CGG
1448 CCGG
NlaIV 657 GGAGCC GG[ACGT]^[ACGT]CC
940 GGATCC
Sau96I 612 GGACC G^G[ACGT]CC
786 GGCCC
ScaI 778 AGTACT AGT^ACT
800 AGTACT
SspI 313 AATATT AAT^ATT
1245 AATATT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
NciI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I