Restriction map for
pFN21AB0443
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# NO CUT ENZYMES ( 140 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HpaI KasI
Ksp632I KspI MaeI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 996 GACGTC GACGT^C
Acc65I 578 GGTACC G^GTACC
AlwNI 391 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 748 CCTGAGG CC^T[ACGT]AGG
Asp718I 578 GGTACC G^GTACC
BbsI 1111 GTCTTC GAAGAC(2/6)
BglI 1028 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 27 GTGCCC G[AGT]GC[ACT]^C
BpmI 793 CTGGAG CTGGAG(16/14)
BpuAI 1111 GTCTTC GAAGAC(2/6)
BsaBI 198 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1286I 27 GTGCCC G[AGT]GC[ACT]^C
BsrBI 607 GAGCGG CCGCTC(-3/-3)
BsrDI 344 GCAATG GCAATG(2/0)
BsrI 864 ACTGG ACTGG(1/-1)
BstAPI 390 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 417 CATCC GGATG(2/0)
BstXI 557 CCAACCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 748 CCTGAGG CC^T[ACGT]AGG
DraII 482 GGGTCCT [GA]G^G[ACGT]CC[CT]
Eco81I 748 CCTGAGG CC^T[ACGT]AGG
EcoO109I 482 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 78 CCAAGG C^C[AT][AT]GG
FokI 417 CATCC GGATG(9/13)
HincII 235 GTCAAC GT[CT]^[GA]AC
HindII 235 GTCAAC GT[CT]^[GA]AC
HindIII 263 AAGCTT A^AGCTT
KpnI 578 GGTACC GGTAC^C
MamI 198 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 401 TTAA T^TAA
MslI 223 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 279 CCGCTG C[AC]G^C[GT]G
NspI 977 GCATGC [GA]CATG^[CT]
PflMI 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 482 GGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1010 AGTACT AGT^ACT
SexAI 299 ACCTGGT A^CC[AT]GGT
SmaI 512 CCCGGG CCC^GGG
SmlI 970 CTCAAG C^T[CT][GA]AG
SphI 977 GCATGC GCATG^C
StuI 839 AGGCCT AGG^CCT
StyI 78 CCAAGG C^C[AT][AT]GG
Tru9I 401 TTAA T^TAA
TspRI 863 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 512 CCCGGG C^CCGGG
XmaI 512 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 1025 CGCG CG^CG
1108 CGCG
AcyI 922 GACGCC G[GA]^CG[CT]C
996 GACGTC
AlwI 204 GATCC GGATC(4/5)
432 GATCC
AvaI 512 CCCGGG C^[CT]CG[GA]G
601 CTCGGG
BalI 527 TGGCCA TGG^CCA
757 TGGCCA
BsaHI 922 GACGCC G[GA]^CG[CT]C
996 GACGTC
BsmFI 443 GGGAC GGGAC(10/14)
611 GGGAC
BsoBI 512 CCCGGG C^[CT]CG[GA]G
601 CTCGGG
BsrFI 121 ACCGGC [GA]^CCGG[CT]
581 ACCGGC
BssSI 223 CACGAG CACGAG(-5/-1)
473 CTCGTG
BstUI 1025 CGCG CG^CG
1108 CGCG
BstYI 203 AGATCC [GA]^GATC[CT]
431 AGATCC
Cfr10I 121 ACCGGC [GA]^CCGG[CT]
581 ACCGGC
DdeI 371 CTGAG C^T[ACGT]AG
749 CTGAG
HgaI 922 GACGC GACGC(5/10)
1109 GCGTC
Hin1I 922 GACGCC G[GA]^CG[CT]C
996 GACGTC
HinfI 16 GACTC G^A[ACGT]TC
1068 GAGTC
MaeII 764 ACGT A^CGT
997 ACGT
MaeIII 568 GTGAC ^GT[ACGT]AC
851 GTGAC
MflI 203 AGATCC [GA]^GATC[CT]
431 AGATCC
MluNI 527 TGGCCA TGG^CCA
757 TGGCCA
MscI 527 TGGCCA TGG^CCA
757 TGGCCA
MvnI 1025 CGCG CG^CG
1108 CGCG
PleI 16 GACTC GAGTC(4/5)
1068 GAGTC
Tsp45I 568 GTGAC ^GT[GC]AC
851 GTGAC
XhoII 203 AGATCC [GA]^GATC[CT]
431 AGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BsaJI BsiYI BslI
BssKI BstNI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I