Restriction map for
pFN21AE6313
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspHI BspMI
BsrGI Bst1107I BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MflI MluI MspA1I MunI
NarI NdeI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 265 GTCTAC GT^[AC][GT]AC
AccIII 902 TCCGGA T^CCGGA
AflIII 380 ACATGT A^C[GA][CT]GT
AlwI 830 GGATC GGATC(4/5)
AscI 30 GGCGCGCC GG^CGCGCC
AspHI 1050 GTGCTC G[AT]GC[AT]^C
BbsI 745 GAAGAC GAAGAC(2/6)
BbvCI 678 CCTCAGC CCTCAGC(-5/-2)
BcgI 744 CGAAGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1214 GGATAC GTATCC(6/5)
BmyI 1050 GTGCTC G[AGT]GC[ACT]^C
BpmI 1170 CTCCAG CTGGAG(16/14)
BpuAI 745 GAAGAC GAAGAC(2/6)
BsaAI 1159 TACGTG [CT]AC^GT[GA]
BsaBI 1215 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 902 TCCGGA T^CCGGA
BseRI 1024 GAGGAG GAGGAG(10/8)
BsgI 345 CTGCAC GTGCAG(16/14)
BsiHKAI 1050 GTGCTC G[AT]GC[AT]^C
BsmI 1081 GAATGC GAATGC(1/-1)
Bsp1286I 1050 GTGCTC G[AGT]GC[ACT]^C
BspEI 902 TCCGGA T^CCGGA
BspLU11I 380 ACATGT A^CATGT
BssHII 31 GCGCGC G^CGCGC
BssSI 728 CACGAG CACGAG(-5/-1)
BstBI 742 TTCGAA TT^CGAA
EcoNI 1233 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 648 ATGCAT ATGCA^T
MamI 1215 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 902 TCCGGA T^CCGGA
MseI 122 TTAA T^TAA
NaeI 17 GCCGGC GCC^GGC
NgoMIV 17 GCCGGC G^CCGGC
NsiI 648 ATGCAT ATGCA^T
NspI 380 ACATGT [GA]CATG^[CT]
NspV 742 TTCGAA TT^CGAA
PflMI 487 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1018 CTGCAG CTGCA^G
SapI 127 GAAGAGC GCTCTTC(1/4)
ScaI 938 AGTACT AGT^ACT
SfuI 742 TTCGAA TT^CGAA
SspI 835 AATATT AAT^ATT
TfiI 1185 GATTC G^A[AT]TC
Tru9I 122 TTAA T^TAA
Tsp45I 300 GTCAC ^GT[GC]AC
Van91I 487 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 139 GAATTT [GA]^AATT[CT]
430 AAATTT
ApoI 139 GAATTT [GA]^AATT[CT]
430 AAATTT
AvaI 170 CCCGAG C^[CT]CG[GA]G
884 CCCGAG
BcnI 49 CCCGG CC^[GC]GG
60 CCGGG
BglI 44 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
632 GCCCCAAGGGC
BsaI 887 GAGACC GGTCTC(1/5)
55 GGTCTC
BsaWI 83 ACCGGA [AT]^CCGG[AT]
902 TCCGGA
BsmAI 887 GAGAC GTCTC(1/5)
56 GTCTC
BsoBI 170 CCCGAG C^[CT]CG[GA]G
884 CCCGAG
BsrBI 442 CCGCTC CCGCTC(-3/-3)
9 GAGCGG
BsrFI 17 GCCGGC [GA]^CCGG[CT]
76 ACCGGC
BstAPI 641 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1155 GCAGTACGTGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
76 ACCGGC
DdeI 527 CTAAG C^T[ACGT]AG
679 CTCAG
DrdI 748 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1168 GACTCCAGGGTC
EarI 512 CTCTTC CTCTTC(1/4)
127 GAAGAG
FauI 287 GCGGG CCCGC(4/6)
1164 GCGGG
HphI 586 GGTGA GGTGA(8/7)
881 TCACC
Ksp632I 512 CTCTTC CTCTTC(1/4)
127 GAAGAG
MaeII 303 ACGT A^CGT
1160 ACGT
MaeIII 300 GTCAC ^GT[ACGT]AC
932 GTAAC
NciI 49 CCCGG CC^[GC]GG
60 CCGGG
PleI 1168 GACTC GAGTC(4/5)
589 GAGTC
PpuMI 62 GGGTCCT [GA]G^G[AT]CC[CT]
667 GGGACCC
StuI 566 AGGCCT AGG^CCT
1103 AGGCCT
TaqI 104 TCGA T^CGA
743 TCGA
TthHB8I 104 TCGA T^CGA
743 TCGA
XbaI 1188 TCTAGA T^CTAGA
1225 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BalI BbvI BfaI BsaJI BsiYI
BslI BsmFI BsrDI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeI MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MvnI MwoI NcoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp509I TspRI