Restriction map for
pFN21AE6337
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco81I EcoRI EcoRV
EcoT14I EcoT22I FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MamI MfeI
MflI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 730 AAATTT [GA]^AATT[CT]
AlwNI 295 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 36 AGCGCT AGC^GCT
ApoI 730 AAATTT [GA]^AATT[CT]
AspHI 244 GAGCAC G[AT]GC[AT]^C
AvaII 870 GGTCC G^G[AT]CC
BalI 326 TGGCCA TGG^CCA
BanII 138 GGGCTC G[GA]GC[CT]^C
BbvCI 393 GCTGAGG CCTCAGC(-5/-2)
BsaI 42 GGTCTC GGTCTC(1/5)
BseRI 280 GAGGAG GAGGAG(10/8)
BsiEI 86 CGGCCG CG[GA][CT]^CG
BsiHKAI 244 GAGCAC G[AT]GC[AT]^C
BspHI 662 TCATGA T^CATGA
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BssSI 569 CACGAG CACGAG(-5/-1)
BstAPI 765 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1132 GGTCACC G^GT[ACGT]ACC
BstF5I 675 GGATG GGATG(2/0)
BstPI 1132 GGTCACC G^GT[ACGT]ACC
DraII 177 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 86 CGGCCG C^GGCCG
EclXI 86 CGGCCG C^GGCCG
Eco47III 36 AGCGCT AGC^GCT
Eco52I 86 CGGCCG C^GGCCG
EcoNI 252 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 177 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1132 GGTCACC G^GT[ACGT]ACC
FauI 627 GCGGG CCCGC(4/6)
FokI 675 GGATG GGATG(9/13)
HaeII 36 AGCGCT [GA]GCGC^[CT]
KspI 89 CCGCGG CCGC^GG
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
MseI 1007 TTAA T^TAA
NheI 372 GCTAGC G^CTAGC
PaeR7I 1079 CTCGAG C^TCGAG
PflMI 936 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 766 CAGCTG CAG^CTG
RcaI 662 TCATGA T^CATGA
SacII 89 CCGCGG CCGC^GG
SexAI 476 ACCTGGT A^CC[AT]GGT
SphI 761 GCATGC GCATG^C
Tru9I 1007 TTAA T^TAA
Tsp509I 731 AATT ^AATT
Van91I 936 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 471 TCTAGA T^CTAGA
XhoI 1079 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 90 CGCG CG^CG
107 CGCG
AvaI 32 CCCGAG C^[CT]CG[GA]G
1079 CTCGAG
BanI 916 GGTGCC G^G[CT][GA]CC
923 GGCACC
BfaI 373 CTAG C^TAG
472 CTAG
BpmI 1023 CTCCAG CTGGAG(16/14)
289 CTGGAG
BsmBI 167 GAGACG CGTCTC(1/5)
910 GAGACG
BsmFI 226 GTCCC GGGAC(10/14)
871 GTCCC
BsoBI 32 CCCGAG C^[CT]CG[GA]G
1079 CTCGAG
BspMI 1136 ACCTGC ACCTGC(4/8)
294 GCAGGT
BsrI 229 CCAGT ACTGG(1/-1)
786 CCAGT
BstUI 90 CGCG CG^CG
107 CGCG
CfoI 37 GCGC GCG^C
94 GCGC
Cfr13I 178 GGGCC G^G[ACGT]CC
870 GGTCC
DsaI 89 CCGCGG C^C[GA][CT]GG
965 CCACGG
HhaI 37 GCGC GCG^C
94 GCGC
HinP1I 37 GCGC G^CGC
94 GCGC
MaeI 373 CTAG C^TAG
472 CTAG
MaeII 125 ACGT A^CGT
422 ACGT
MboII 558 GAAGA GAAGA(8/7)
1047 GAAGA
MvnI 90 CGCG CG^CG
107 CGCG
NspI 761 GCATGC [GA]CATG^[CT]
992 ACATGC
PstI 688 CTGCAG CTGCA^G
1138 CTGCAG
Sau96I 178 GGGCC G^G[ACGT]CC
870 GGTCC
SmlI 1012 CTTGAG C^T[CT][GA]AG
1079 CTCGAG
TaqI 489 TCGA T^CGA
1080 TCGA
TfiI 342 GATTC G^A[AT]TC
777 GAATC
TthHB8I 489 TCGA T^CGA
1080 TCGA
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AfaI AluI BbvI BclI
BcnI BmyI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BssKI BstNI
Cac8I DdeI DpnII EaeI EcoRII
FbaI Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI ScrFI SfaNI
SfcI TseI Tsp45I TspRI