Restriction map for
pFN21AE4402
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI AocI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO109I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MseI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 746 GAATTT [GA]^AATT[CT]
AcyI 1193 GGCGTC G[GA]^CG[CT]C
AflIII 564 ACGTGT A^C[GA][CT]GT
Alw44I 982 GTGCAC G^TGCAC
AlwNI 638 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 148 AGCGCT AGC^GCT
ApaLI 982 GTGCAC G^TGCAC
ApoI 746 GAATTT [GA]^AATT[CT]
Asp700I 765 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 982 GTGCAC G[AT]GC[AT]^C
AspI 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BlpI 1002 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1002 GCTAAGC GC^T[ACGT]AGC
BsaHI 1193 GGCGTC G[GA]^CG[CT]C
BsaWI 99 ACCGGA [AT]^CCGG[AT]
BsiEI 97 CGACCG CG[GA][CT]^CG
BsiHKAI 982 GTGCAC G[AT]GC[AT]^C
BspDI 1159 ATCGAT AT^CGAT
BsrBI 65 GAGCGG CCGCTC(-3/-3)
BssSI 740 CTCGTG CACGAG(-5/-1)
BstAPI 208 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 598 GGTGACC G^GT[ACGT]ACC
BstPI 598 GGTGACC G^GT[ACGT]ACC
CelII 1002 GCTAAGC GC^T[ACGT]AGC
CfoI 149 GCGC GCG^C
ClaI 1159 ATCGAT AT^CGAT
DraIII 581 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 148 AGCGCT AGC^GCT
EcoNI 867 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 598 GGTGACC G^GT[ACGT]ACC
EcoRV 1213 GATATC GAT^ATC
EcoT14I 815 CCATGG C^C[AT][AT]GG
HaeII 148 AGCGCT [GA]GCGC^[CT]
HhaI 149 GCGC GCG^C
Hin1I 1193 GGCGTC G[GA]^CG[CT]C
HinP1I 149 GCGC G^CGC
MslI 754 CACAACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 80 CCGCTG C[AC]G^C[GT]G
NcoI 815 CCATGG C^CATGG
PflFI 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 242 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 707 CTGCAG CTGCA^G
SapI 765 GAAGAGC GCTCTTC(1/4)
SmaI 36 CCCGGG CCC^GGG
StuI 864 AGGCCT AGG^CCT
StyI 815 CCATGG C^C[AT][AT]GG
TfiI 854 GATTC G^A[AT]TC
Tth111I 515 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 242 CCACTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 36 CCCGGG C^CCGGG
XmaI 36 CCCGGG C^CCGGG
XmnI 765 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 7 GGTCC G^G[AT]CC
1116 GGACC
BmyI 960 GGGCAC G[AGT]GC[ACT]^C
982 GTGCAC
BsaI 337 GAGACC GGTCTC(1/5)
71 GGTCTC
Bsp1286I 960 GGGCAC G[AGT]GC[ACT]^C
982 GTGCAC
BstXI 891 CCAAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1050 CCAAAAGCGTGG
BstYI 426 GGATCT [GA]^GATC[CT]
1028 AGATCC
Cac8I 31 GCCCGC GC[ACGT]^[ACGT]GC
532 GCTTGC
Cfr13I 7 GGTCC G^G[ACGT]CC
1116 GGACC
DsaI 422 CCACGG C^C[GA][CT]GG
815 CCATGG
EarI 765 GAAGAG CTCTTC(1/4)
837 GAAGAG
FauI 32 CCCGC CCCGC(4/6)
67 GCGGG
HgaI 252 GACGC GACGC(5/10)
1194 GCGTC
Ksp632I 765 GAAGAG CTCTTC(1/4)
837 GAAGAG
MboII 765 GAAGA GAAGA(8/7)
837 GAAGA
MflI 426 GGATCT [GA]^GATC[CT]
1028 AGATCC
PaeR7I 539 CTCGAG C^TCGAG
722 CTCGAG
Sau96I 7 GGTCC G^G[ACGT]CC
1116 GGACC
SfcI 618 CTATAG C^T[GA][CT]AG
707 CTGCAG
SmlI 539 CTCGAG C^T[CT][GA]AG
722 CTCGAG
Tsp45I 194 GTCAC ^GT[GC]AC
599 GTGAC
Tsp509I 747 AATT ^AATT
1111 AATT
XbaI 429 TCTAGA T^CTAGA
484 TCTAGA
XhoI 539 CTCGAG C^TCGAG
722 CTCGAG
XhoII 426 GGATCT [GA]^GATC[CT]
1028 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BalI BbvI BciVI BcnI BfaI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI BsrI BssKI
BstF5I BstNI DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfaNI TaqI TseI
TspRI TthHB8I