Restriction map for
pFN21AE4331
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BanII BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TfiI Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 174 CGCG CG^CG
AcyI 763 GGCGCC G[GA]^CG[CT]C
Alw44I 633 GTGCAC G^TGCAC
AlwNI 731 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 28 AGCGCT AGC^GCT
ApaLI 633 GTGCAC G^TGCAC
AspHI 633 GTGCAC G[AT]GC[AT]^C
AspI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 763 GGCGCC G^G[CT][GA]CC
BbeI 763 GGCGCC GGCGC^C
BfaI 88 CTAG C^TAG
BglII 198 AGATCT A^GATCT
BsaHI 763 GGCGCC G[GA]^CG[CT]C
BsaI 214 GAGACC GGTCTC(1/5)
BsaWI 426 TCCGGT [AT]^CCGG[AT]
BsiHKAI 633 GTGCAC G[AT]GC[AT]^C
Bsp1407I 479 TGTACA T^GTACA
BsrGI 479 TGTACA T^GTACA
BsrI 679 ACTGG ACTGG(1/-1)
BstAPI 730 GCAGGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 174 CGCG CG^CG
BstXI 353 CCACGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 91 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 749 CCATGG C^C[GA][CT]GG
Eco47III 28 AGCGCT AGC^GCT
EcoO109I 91 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 169 GATATC GAT^ATC
FauI 767 CCCGC CCCGC(4/6)
HgaI 413 GCGTC GACGC(5/10)
Hin1I 763 GGCGCC G[GA]^CG[CT]C
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
HphI 147 GGTGA GGTGA(8/7)
KasI 763 GGCGCC G^GCGCC
MaeI 88 CTAG C^TAG
MaeII 578 ACGT A^CGT
MvnI 174 CGCG CG^CG
NarI 763 GGCGCC GG^CGCC
NcoI 749 CCATGG C^CATGG
PaeR7I 569 CTCGAG C^TCGAG
PflFI 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfoI 763 GGCGCC GGC^GCC
SmaI 326 CCCGGG CCC^GGG
SmlI 569 CTCGAG C^T[CT][GA]AG
SspBI 479 TGTACA T^GTACA
StuI 971 AGGCCT AGG^CCT
Tsp509I 433 AATT ^AATT
Tth111I 517 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 740 CCACCCTCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 569 CTCGAG C^TCGAG
XmaCI 326 CCCGGG C^CCGGG
XmaI 326 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 480 GTAC GT^AC
854 GTAC
AvaII 244 GGACC G^G[AT]CC
660 GGACC
BalI 362 TGGCCA TGG^CCA
594 TGGCCA
BamHI 561 GGATCC G^GATCC
686 GGATCC
BmyI 383 GTGCCC G[AGT]GC[ACT]^C
633 GTGCAC
BpmI 98 CTGGAG CTGGAG(16/14)
299 CTGGAG
BseRI 924 CTCCTC GAGGAG(10/8)
934 GAGGAG
BsmAI 214 GAGAC GTCTC(1/5)
922 GTCTC
Bsp1286I 383 GTGCCC G[AGT]GC[ACT]^C
633 GTGCAC
BsrDI 417 CATTGC GCAATG(2/0)
793 GCAATG
BsrFI 697 GCCGGC [GA]^CCGG[CT]
760 GCCGGC
Cfr10I 697 GCCGGC [GA]^CCGG[CT]
760 GCCGGC
DdeI 202 CTGAG C^T[ACGT]AG
629 CTCAG
HaeII 28 AGCGCT [GA]GCGC^[CT]
763 GGCGCC
HinfI 302 GAGTC G^A[ACGT]TC
572 GAGTC
MaeIII 505 GTGAC ^GT[ACGT]AC
837 GTCAC
MluNI 362 TGGCCA TGG^CCA
594 TGGCCA
MscI 362 TGGCCA TGG^CCA
594 TGGCCA
MseI 431 TTAA T^TAA
833 TTAA
MslI 354 CACGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
702 CACGGAGGTG
NaeI 697 GCCGGC GCC^GGC
760 GCCGGC
NgoMIV 697 GCCGGC G^CCGGC
760 GCCGGC
PleI 302 GAGTC GAGTC(4/5)
572 GAGTC
PvuII 253 CAGCTG CAG^CTG
664 CAGCTG
RsaI 480 GTAC GT^AC
854 GTAC
Tru9I 431 TTAA T^TAA
833 TTAA
Tsp45I 505 GTGAC ^GT[GC]AC
837 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaI BbsI
BbvI BcnI BpuAI BsaJI BsiYI
BslI BsmFI BsoBI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI TspRI TthHB8I XhoII